Biosynthesis of metallic nanoparticles by bacterial cell-free extract.

The biosynthesis of metallic nanoparticles (MNPs), encompassing noble metals, metal oxides, and sulfides, has gained significant attention in recent years due to their unique properties and wide-ranging applications. However, traditional chemical synthesis methods often involve extreme conditions, harsh chemicals, and negative environmental impacts. Consequently, developing a simple, non-toxic, and eco-friendly approach for MNP synthesis is paramount. One promising method that addresses these concerns is using a bacterial cell-free extract (CFE) as a mediator for biosynthesis. Compared with other biosynthesis production methods, the purification process of MNPs synthesized using bacterial CFEs is much simpler, and CFE production is easier to standardize and reproduce. Bacterial CFEs are rich in various biomolecules, including proteins, enzymes, and peptides, which serve as both reducing and oxidizing agents during MNP formation. These biomolecules act as capping agents, contributing to the stability and monodisperse nature of MNPs. Using bacterial CFEs for MNP synthesis offers several advantages. Firstly, it aligns with eco-friendly practices as a biosynthesis approach. The non-toxic process minimizes environmental damage. Additionally, bacterial CFEs are cost-effective, making large-scale production economically viable. This review provides insights into these mechanisms, highlighting the role of CFE biomolecules and their impact on MNP characteristics. It also investigates the correlation between synthesis parameters, morphologies, and physical, chemical, and biological properties, allowing for tailored MNP design through the biosynthesis conditions. Despite its advantages, bacterial CFE-mediated biosynthesis faces challenges. This review addresses these challenges and discusses potential solutions. It also explores future perspectives, emphasizing areas for further investigation and innovation. In summary, using bacterial CFEs to synthesize MNPs offers significant advantages over other methods. It ensures eco-friendly, non-toxic, and cost-effective production. The review emphasizes the mechanisms and biomolecules involved, showcasing the potential for tailored MNP design. It also addresses challenges and prospects, paving the way for advancements in this field. Furthermore, the originality of this work lies in the exploitation of bacterial CFEs as a highly efficient and scalable platform for MNP synthesis.

Enhanced production of surfactin using cassava wastewater and hydrophobic inducers: a prospection on new homologues.

Biosurfactants are amphipathic molecules that can be applied in a wide range of areas. The cost of production limits the industrial application of biosurfactants. Nevertheless, the biosurfactant productivity can be easily enhanced by inducers. This work aimed to investigate the effect of hydrophobic inducers on surfactin production by B. subtilis ATCC 6633 using cassava wastewater as low-cost culture medium. The submerged cultivation was carried out at 30 °C, 150 rpm for 72 h. The fermentation parameters used were bacterial growth, consumption of sugars, and surfactin production, including surfactin homologues. The surface tension decreased by 40% after 12 h, when compared to control. Depletion of sugars was observed in all experiments. Palmitic acid led to the highest yield in terms of surfactin production (≈ 1.3 g·L- 1 of pure surfactin). The inducers triggered the production of new surfactin homologues, that represent, potentially, new biological activities.

Biopolymers as green-based food packaging materials: A focus on modified and unmodified starch-based films.

The ideal food packaging materials are recyclable, biodegradable, and compostable. Starch from plant sources, such as tubers, legumes, cereals, and agro-industrial plant residues, is considered one of the most suitable biopolymers for producing biodegradable films due to its natural abundance and low cost. The chemical modification of starch makes it possible to produce films with better technological properties by changing the functional groups into starch. Using biopolymers extracted from agro-industrial waste can add value to a raw material that would otherwise be discarded. The recent COVID-19 pandemic has driven a rise in demand for single-use plastics, intensifying pressure on this already out-of-control issue. This review provides an overview of biopolymers, with a particular focus on starch, to develop sustainable materials for food packaging. This study summarizes the methods and provides a potential approach to starch modification for improving the mechanical and barrier properties of starch-based films. This review also updates some trends pointed out by the food packaging sector in the last years, considering the impacts of the COVID-19 pandemic. Perspectives to achieve more sustainable food packaging toward a more circular economy are drawn.

Enhanced antimicrobial and antioxidant capacity of Thymus vulgaris, Lippia sidoides, and Cymbopogon citratus emulsions when combined with mannosylerythritol a lipid biosurfactant.

Essential oils (EOs) are natural and effective agents for controlling microorganisms which cause biodeterioration and disease. However, their application is hampered/restricted due to hydrophobicity and rapid vaporization of these compounds. Encapsulation technology provides an effective approach to maintain EO stabilization and prevent the loss of volatile ingredients. Meanwhile, using a synthetic surfactant is seen as counter-productive; therefore, a natural biosurfactant is more reasonable and can potentially increase activity due to its other biological proprieties. This work aims to evaluate the mannosylerythritol lipid (MEL) biosurfactant combined with Thymus vulgaris, Lippia sidoides, and Cymbopogon citratus essential oil emulsions (O/W) and evaluate its antimicrobial and antioxidant capacity. The biosurfactant MEL demonstrated activity against Bacillus subtilis and Penicillium sp. After emulsification, the antimicrobial activity of Thymus vulgaris and Lippia sidoides was increased against Escherichia coli (500 µg/mL), Staphylococcus aureus (600 µg/mL), Bacillus subtilis (120 µg/mL), Pseudomonas aeruginosa (1500 µg/mL), Penicillium sp. (62.25 µg/mL), Aspergillus flavus (250 µg/mL), Fusarium oxysporum (100 and 250 µg/mL), and Candida albicans (125 and 250 µg/mL). We report that emulsions prepared with MEL have high inhibitory activity, maintain the active concentration, and increase antioxidant capacity by 7.33% (Thymus vulgaris), 13.71% (Lippia sidoides), and 3.15% (Cymbopogon citratus).

Mannosylerythritol lipids as green pesticides and plant biostimulants.

Biosurfactants can be applied in the formulation of personal care products, as food additives, and as biocontrol agents in the agricultural sector. Glycolipids and lipopeptides represent an important group of microbial-based biosurfactants with biostimulating properties. Among them, the mannosylerythritol lipids also presented antimicrobial activity, mostly against Gram-positive bacteria and phytopathogenic fungi. In this sense, mannosylerythritol lipids are a potential safer green alternative for partially replacing synthetic pesticides. This review aimed to critically discuss the current state of the art and future trends of mannosylerythritol lipids as green pesticides and biostimulants for seed germination and plant growth. Due to their chemical structure, mannosylerythritol lipids are likely related to energy pathways such as glycolysis and Krebs cycle, i.e. a direct cellular biostimulant potential. In this case, experimental evidence from other glycolipids indicated that structural and chemical changes as a potential drug vehicle due to morphological changes caused by biosurfactant-membrane interaction. In addition, like other biosurfactants, mannosylerythritol lipids can trigger self-defense mechanisms, leading to a lower frequency of phytopathogen infections. Therefore, mannosylerythritol lipids have the potential for biostimulation and antiphytopathogenic action, despite that to date no data are available on mannosylerythritol lipids as biostimulants and green pesticides simultaneously. Based on the current state of the art, mannosylerythritol lipids have great potential for a biotechnological advance toward more sustainable agriculture. © 2022 Society of Chemical Industry.

Cassava starch films for food packaging: Trends over the last decade and future research.

Cassava starch is one of the most available and cost-effective biopolymers. This work aimed to apply a bibliometric methodology to identify the most impactful scientific data on cassava starch and its residues for food packaging in the last ten years. As a result, an increasing interest in this subject has been observed, mainly in the past five years. Among the 85 selected scientific publications, Brazil and China have been leading the research on starch-based films, accounting for 39 % of the total. The International Journal of Biological Macromolecules was the main scientific source of information. Besides cassava starch, 41.18 % of these studies added other biopolymers, 5.88 % added synthetic polymers, and 4.71 % added a combination of both. Studies analyzed suggested that different modifications in starch can improve films' mechanical and barrier properties. In addition, 52.94 % of articles evaluated the film's bioactivity. Still, only 37.65 % assessed the performance of those films as food packaging, suggesting that more studies should be conducted on assessing the potential of these alternative packages. Future research should consider scale-up methods for film production, including cost analysis, assessment life cycle, and the impact on the safety and quality of a broader range of foods.

Copper recovery through biohydrometallurgy route: chemical and physical characterization of magnetic (m), non-magnetic (nm) and mix samples from obsolete smartphones.

The more modern electronics are, the smaller and complex printed circuit boards are. Thus, these materials are continually changed (physicochemically), increasing the copper concentrations in smartphones. In this sense, it is challenging to set standardized recycling processes to improve metal recovery. In addition, biohydrometallurgy is a clean and cheap process to obtain critical metals from low-grade sources and waste electronic equipment. Therefore, the aim of this work was to characterize, physicochemically, 21 PCBs from smartphones manufactured from 2010 to 2015, and then to recover the copper by Acidithiobacillus ferrooxidans (biohydrometallurgy). The PCBs were comminuted and separated into Magnetic (M), Nonmagnetic (NM) and without magnetic separation (MIX) samples. It was identified 217.8; 560.3 and 401.3 mg Cu/g of PCBs for M, NM and MIX samples, respectively. Regarding biohydrometallurgy, the culture media iron-supplemented (NM + Fe and MIX + Fe) increased the copper content by 2.6 and 7.2%, respectively, and the magnetic separation step was insignificant.

Cassava wastewater valorization for the production of biosurfactants: surfactin, rhamnolipids, and mannosileritritol lipids.

The global production of cassava was estimated at ca. 303 million tons. Due to this high production, the cassava processing industry (cassava flour and starch) generates approximately ca. 0.65 kg of solid residue and ca. 25.3 l of wastewater per kg of fresh processed cassava root. The composition of the liquid effluent varies according to its origin; for example, the effluent from cassava flour production, when compared to the wastewater from the starch processing, presents a higher organic load (ca. 12 times) and total cyanide (ca. 29 times). It is worthy to highlight the toxicity of cassava residues regarding cyanide presence, which could generate disorders with acute or chronic symptoms in humans and animals. In this sense, the development of simple and low-cost eco-friendly methods for the proper treatment or reuse of cassava wastewater is a challenging, but promising path. Cassava wastewater is rich in macro-nutrients (proteins, starch, sugars) and micro-nutrients (iron, magnesium), enabling its use as a low-cost culture medium for biotechnological processes, such as the production of biosurfactants. These compounds are amphipathic molecules synthesized by living cells and can be widely used in industries as pharmaceutical agents, for microbial-enhanced oil recovery, among others. Amongst these biosurfactants, surfactin, rhamnolipids, and mannosileritritol lipids show remarkable properties such as antimicrobial, biodegradability, demulsifying and emulsifying capacity. However, the high production cost restricts the massive biosurfactant applications. Therefore, this study aims to present the state of the art and challenges in the production of biosurfactants using cassava wastewater as an alternative culture medium.

Chlorella vulgaris phycoremediation at low Cu+2 contents: Proteomic profiling of microalgal metabolism related to fatty acids and CO2 fixation.

The aim of this work was to correlate metabolic changes with copper ions (Cu+2) bioremediation by microalgae C. vulgaris 097 CCMA-UFSCar at low Cu+2 content. The metabolic effects include proteome changes related to fatty acid biosynthesis (value-added product) and carbon fixation (climate change mitigation). Cu+2, even at low concentration, showed a significant negative impact on C. vulgaris growth. The microalgal bioremediation reached 100, 74, 38 and 26% for Cu+2 content at 0.1; 0.3; 0.6 and 0.9 mg L-1, respectively. Regarding proteomics, the numbers of proteins reduced (≈37%) from 581 proteins (control) to 369 proteins (0.9 mg of Cu+2 L-1) compared to control. The microalgal CO2 fixation is strictly related to the Calvin cycle, particularly phase 1, in which ribulose bisphosphate carboxylase large chain (RuBisCO) produces two phosphoglycerate molecules from CO2 and ribulose 1,5-bisphosphate. Then, phosphoglycerate can be metabolically reduced into glucose. When compared to control, the RuBisCO was underexpressed (≈50%). Similar changes in proteomic profiling of metabolism-related to fatty acid biosynthesis was observed. Nevertheless, no protein was found for the cultivation at 0.9 mg of Cu+2 L-1. Thus, the analysis of C. vulgaris proteomic data indicated that even at low concentration, Cu+2 lead to drastic metabolic changes.

Biodegradation of azo dye-containing wastewater by activated sludge: a critical review.

The effluent from the textile industry is a complex mixture of recalcitrant molecules that can harm the environment and human health. Biological treatments are usually applied for this wastewater, particularly activated sludge, due to its high efficiency, and low implementation and operation costs. However, the activated sludge microbiome is rarely well-known. In general, activated sludges are composed of Acidobacteria, Bacillus, Clostridium, Pseudomonas, Proteobacteria, and Streptococcus, in which Bacillus and Pseudomonas are highlighted for bacterial dye degradation. Consequently, the process is not carried out under optimum conditions (treatment yield). Therefore, this review aims to contextualize the potential environmental impacts of azo dye-containing wastewater from the textile industry, including toxicity, activated sludge microbiome identification, in particular using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a novel, rapid and accurate strategy for the identification of activated sludge microbiome (potential to enhance treatment yield).

Biosurfactant inducers for enhanced production of surfactin and rhamnolipids: an overview.

Biosurfactants can be widely used in industries as pharmaceutical agents, for microbial enhanced oil recovery, crop biostimulation, among others. Surfactin and rhamnolipids are well-known biosurfactants. These compounds have several advantages over chemical surfactants, however they are not economically competitive, since their production cost is up to 12 times higher than chemical surfactants. In this sense, an interesting approach is to replace synthetic culture medium, which represents ≈ 30% of the production cost by agro-industrial wastes. In addition, biosurfactant productivity can be easily enhanced by inductor supplementation into culture medium that triggers biosurfactant metabolism. Biosurfactant inducers are mainly a pool of hydrophobic molecules (e.g. olive oil-saturated and unsaturated fatty acids, proteins and vitamins). Nevertheless, there is little information on inducer effects of specific molecules (e.g. oleic acid). In general, hydrophobic inducers lead to higher fatty acid chain lengths (biosurfactant chemical structure). Therefore, the aim of this review was to critically discuss the current state of the art and future trends on biosurfactant production, in particular biosurfactant inducers. Taking into account the last 10 years, there is a clear lack of information on correlation between "inducers" or "hydrophobic inducers" AND "biosurfactants", since only 13 documents were found (Scopus database). Thus, it is essential to deeply investigate all inducer effects on biosurfactant production, mainly yield and chemical structure.

Biological activity of mannosylerythritol lipids on the mammalian cells.

Mannosylerythritol lipids (MEL) are glycolipids mainly produced by pseudo-yeasts. These molecules present remarkable biological activities widely explored in many fields, including medicine, pharmaceuticals, and cosmetics. This review presents the main biological activity of MEL on the HL60, K562, B16, PC12, and skin cells. There is strong evidence that MEL changes the levels of glycosphingolipids of HL-60 lineage, which induce differentiation into granulocytic cells. Regarding B16 cells, MEL can trigger both apoptosis (10 µM) and cell differentiation (5 µM), in which the MEL concentration is related to each metabolic pathway. MEL can also trigger differentiation in PC12 cells due to the increase in the GalCer content. In this specific case, the effects are transient, and the differentiated cells are unstable and tend to apoptosis. MEL-B can particularly maintain skin hydration and moisture due to their self-assembled structures that resemble the tissue cells. Moreover, MEL-B repair aquaporin expression in the HaCaT keratinocytes damaged with UVA irradiation, whereas MEL-C suppresses the expression of COX-2 protein in fibroblasts, indicating that these glycolipids activate the cellular antioxidant mechanism. Recent findings denoted the anti-melanogenic activity of MEL since they suppress tyrosinase enzyme at mRNA levels in B16 and NHMs cells. MEL act effectively on mammalian cells; however, there is no clear pattern of their metabolic effects. Also, gene expression levels seem to be related to two main factors: chemical structure and concentration. However, the specific signaling cascades that are induced by MEL remain inconclusive. Thus, further investigations are vital to understanding these mechanisms clearly. KEY POINTS: • The four MEL homologs promote different biological responses in mammalian cells. • MEL modifies the pattern of glycosphingolipids in the plasma membrane of tumor cells. • Activation/deactivation of phosphorylation of serine/threonine kinase proteins.

Fruits and vegetable-processing waste: a case study in two markets at Rio de Janeiro, RJ, Brazil.

Fruits and vegetables (FV) consumed in natura or processed produce a significant volume of waste, causing an economic deficit in the productive chain. FV markets feature a significant production of vegetable residues with potential of use, since they commercialize an increasing amount of minimally processed vegetables and fruit juices. To this end, it is important to identify, quantify, and characterize these wastes and to propose feasible and coherent alternatives for their use at regional and worldwide levels. In this paper, a case study of two FV markets in Rio de Janeiro, Brazil, was conducted to identify and quantify FV processing waste. Over a period of 20 days, the FV residues from 31 vegetables and 17 fruits were identified and weighed. It is estimated by extrapolation that 106,000 kg of FV were processed in 1 year in two units of FV markets and 48.6% of FV were discarded as by-products. This may be a consequence of factors that contribute to waste generation, such as the low preparation and/or training of the manipulators as well as the quality of the equipment and the maintenance thereof. Thus, studies that aim to understand the environmental impact by monitoring the of FV waste are fundamental, since this waste can be used as raw material and converted into value-added products.

Mannosylerythritol lipids: antimicrobial and biomedical properties.

Mannosylerythritol lipids (MELs) have attracted particular interest of medical, pharmaceutical, and cosmetic fields, due to their specific characteristics, including non-toxicity, easy biodegradability, and environmental compatibility. Therefore, this review aims to highlight recent findings on MEL biological properties, focusing on issues related to therapeutic applications. Among the main findings is that MELs can play a fundamental role due to their antimicrobial properties against several nosocomial pathogen microorganisms. Other remarkable biological properties of MELs are related to skincare, as antiaging (active agent), and in particular on recover of skin cells that were damaged by UV radiation. MEL is also related to the increased efficiency of DNA transfection in liposome systems. Regarding the health field, these glycolipids seem to be associated with disturbance in the membrane composition of cancerous cells, increasing expression of genes responsible for cytoplasmic stress and apoptosis. Moreover, MELs can be associated with nanoparticles, as a capping agent, also acting to increase the solubility and cytotoxicity of them. Furthermore, the differences in the chemical structure of MEL could improve and expand their biochemical diversity and applications. Such modifications could change their interfacial properties and, thus, reduce the surface tension value, enhance the solubility, lower critical micelle concentrations, and form unique self-assembly structures. The latest is closely related to molecular recognition and protein stabilization properties of MEL, that is, essential parameters for their effective cosmetical and pharmaceutical effects. Thus, this current research indicates the huge potential of MEL for use in biomedical formulations, either alone or in combination with other molecules.

Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis.

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.