RESUMO
Se realizó el seguimiento durante un año de cuatro pacientes que accidentalmente se expusieron por via percutánea a sangre de pacientes infectados con VIH/SIDA con el objetivo de prevenir la infeccion por VIH. Los pacientes fueron evaluados clínicamente y a través de pruebas de laboratorio como la determinación de anticuerpos contra VIH. Los pacientes recibieron tratamiento antiretrovial según esquema establecido. Ninguno de los pacientes seroconvirtió.
Assuntos
Humanos , Masculino , Feminino , HIV , Antibioticoprofilaxia/instrumentação , Antibioticoprofilaxia/métodos , Antibioticoprofilaxia/normas , Antibioticoprofilaxia/tendências , Síndrome da Imunodeficiência Adquirida/diagnósticoRESUMO
Tomando en cuenta que las técnicas de detección de anticuerpo para VIH en general requieren manipulación de sangre por tanto generación de material de desecho potencialmente infectante, se realizó un estudio buscando anticuerpos anti VIH empleando como muestra saliva, los resultados obtenidos muestran que podría ser un método alternativo y seguro para el diagnóstico de infección por VIH
Assuntos
Humanos , HIV , Saliva , Manejo de Espécimes , Síndrome da Imunodeficiência Adquirida , Bolívia , Anticorpos Anti-HIVRESUMO
A group of 10 patients with recently diagnosed pulmonary TB were studied and compared to 10 bacillus Calmette-Guérin (BCG) immunized healthy individuals. Cellular immune mechanisms were explored in vitro utilizing fresh and precultured peripheral blood mononuclear cells exposed to PHA, PPD, and recall antigens (SK/SD and CA). Proliferative assays were also carried out in the presence of either each patient's serum (autologous serum) or cocultured with CD3(+)-depleted adherent cells. Serum measurements of soluble interleukin-2 (IL-2) receptor and synthesis of IL-2 generated by mononuclear cells stimulated with PPD and SK/SD were also performed. Patient sera were able to inhibit autologous as well as allogeneic cell responses, and a significant adherent cell suppressive effect was observed. As a whole the group of patients showed decreased blast transformation to PPD, preserved proliferative responses to other recall antigens, and a low PPD-induced generation of IL-2. Furthermore, as possible evidence of preactivated T cells, these patients demonstrated high soluble IL-2 receptor serum levels. Early compromise of specific cell-mediated immunity, including IL-2 abnormalities, may be of significance in newly diagnosed pulmonary TB.