Metabolic response of broiler chickens to different doses of Enterococcus faecium ICIS 96 in the diet.

The competitiveness and profitability of the poultry industry in market conditions are hampered by growing problems with the safety and quality of poultry meat. The use of targeted microbial preparations can assist in resolving these problems. Numerous studies of the properties of bacteria of the genus Enterococcus have shown their effectiveness in the practice of poultry farming. The objectives of this study were to identify the effect of various doses of Enterococcus faecium ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of E. faecium ICIS 96 containing 1 × 108 cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of live weight per day. The results demonstrated that 0.1 mL of a 1 × 108 per mL enterococcus culture suspension in the poultry diet per 1 kg of live weight per day intensified metabolism and increased the live weight of chickens after 40 d of treatment.

La compétitivité et la rentabilité de l'industrie de la volaille dans les conditions du marché sont désavantagées par les problèmes croissants de sécurité et de qualité de la viande de volaille. La solution à ce problème peut être trouvée grâce à l'utilisation de préparations microbiennes ciblées. De nombreuses études sur les propriétés des bactéries du genre Enterococcus suggèrent leur efficacité dans la pratique de l'aviculture. Le but de l'étude est d'évaluer l'effet de différentes doses d'E. faecium ICIS 96 sur le métabolisme et les qualités productives des poulets à griller. L'expérience a été réalisée sur 72 poulets de chair croisés Cobb-500, répartis en 3 groupes. Les poulets du premier groupe expérimental ont reçu une suspension de culture E. faecium ICIS 96, préparée à raison de 1 milliard de cellules microbiennes dans 1 mL de solution saline. Les poulets de chair du deuxième groupe expérimental ont reçu une dose doublée d'entérocoques, soit 0,2 mL d'une suspension de 1 milliard de cellules microbiennes pour 1 kg de poids vif par jour. Les résultats ont démontré que 0,1 mL d'une suspension de culture d'entérocoques dans l'alimentation des volailles pour 1 kg de poids vif par jour intensifiait le métabolisme et, par conséquent, augmentait le poids vif des poulets dans les 40 jours suivant l'ontogenèse post-incubation.(Traduit par Docteur Serge Messier).

Comparative effectiveness of immune system stimulators in calves' vaccination.

The work aimed to study the comparative effectiveness of the immunostimulants "Roncoleukin" and propolis milk for increasing the nonspecific and specific resistance and immunological reactivity of calves during vaccination against salmonellosis. A study of the "Roncoleukin" effect combined with propolis milk on the biochemical, immunological, and serological parameters of calves during vaccination against salmonellosis was carried out. The objects of research were calves from birth to 2 months of age of the Simmental breed. According to the principle of analogs, they were divided into four groups (control and three experimental), six heads each. All animals at 10 days of age were vaccinated against salmonellosis. The animals of the first experimental group were injected subcutaneously with "Roncoleukin" at birth. Then "Roncoleukin" was injected during vaccination against salmonellosis. The calves of the second experimental group were drunk with propolis milk before feeding. The animals of the third experimental group were drunk with propolis milk before feeding; "Roncoleukin" was injected during vaccination. The use of "Roncoleukin" and propolis milk contributes to correcting immunological parameters and the protein spectrum of the blood during vaccination. The most pronounced changes are observed in calves of the first and third experimental groups. "Roncoleukin" and propolis milk contribute to forming an active specific immune response in calves during vaccination against salmonellosis, which is expressed in an increase in the titer of anti-salmonella antibodies. The maximum antibody titer level was established in calves of the first and third experimental groups, three times higher than the control value.

Immunomodulatory effect of the combined use of Vetosporin Zh probiotic and Gumi-malysh biologically active additive.

BACKGROUND AND AIM: Various means and methods, including probiotics and biologically active additives, have been developed and proposed for production to increase the immunobiological reactivity of the body, regardless of the etiology of its decrease. This study aimed to find out the immune status of calves during the preweaning period in association with Vetosporin Zh, Normosil, and Gumi-malysh. MATERIALS AND METHODS: The research object was 30-day-old calves of black-and-white Holstein breed. The calves were divided into four groups of 20 heads each. The calves of the first, second, and third experimental groups were treated with Normosil probiotic, Vetosporin Zh probiotic, and Vetosporin Zh probiotic in combination with Gumi-malysh, respectively. The calves in the first, second, and third experimental groups were treated with Normosil probiotic, Vetosporin Zh probiotic, and Vetosporin Zh probiotic in combination with Gumi-malysh, respectively. RESULTS: On days 10 and 21 of the experiment, animal blood was collected to determine the content of total protein, protein fractions, immunoglobulins, T and B lymphocytes, phagocytic activity and a phagocytic number of neutrophils, and circulating immune complexes (CIC). The combined use of Vetosporin Zh probiotic (dose, 20 mL) with Gumi-malysh (dose, 30 mL) per animal for 30 days in 1-month-old calves contributes to the increase in the number of T lymphocytes, B lymphocytes, and immunoglobulin A (IgA) and immunoglobulin G (IgG) levels by 2.9%, 3.8%, and 0.96 and 2 g/L, respectively, while reducing the immunoglobulin M (IgM) level; an increase in the phagocytic activity of blood neutrophils and the phagocytic number by 7% and 1.8%, respectively, as well as a decrease in the CIC level with similar indicators in calves that were not treated with the agents. CONCLUSION: The method used in the current study helps increase the number of T and B lymphocytes, increase IgA and IgG levels while reducing IgM levels, and increase the phagocytic activity and a phagocytic number of blood neutrophils, as well as decrease the CIC level.

Influence of interferon-based drugs on immunological indices in specific prevention.

AIM: The research aimed to study the effect of interferon (IFN)-based drugs on the behavior of immunological parameters in calves during the specific prevention of associative infections. MATERIALS AND METHODS: The object of research was 45 black motley cows and their calves from birth to 2 months of life. Serum and colostrum samples were screened for antibodies against Rotavirus, diarrhea, and coronavirus using serological methods. The testing was performed before vaccination, 40 days before calving, 20 days before calving, and before calving. Colostrum samples were taken during the first milk yield. Serum samples from calves were drawn before colostrum feeding as well as at 7, 14, and 21 days, and 1 and 2 months of age. To measure the level of immunoglobulins A, M, and G, additional serum samples were collected from calves at 25, 35, 65, and 75 days after birth. RESULTS: Giving pregnant cows, an IFN-based drug at a dose of 1 ml/kg 48 h before vaccination results in the development and accumulation of antibodies to Rotavirus, coronavirus, and viral diarrhea (VD) in the colostrum, with a titer of 7.6±0.3 log2, 5.8±0.34 log2, and 4.4±0.18 log2, respectively. It indicates an increase in the antigenic activity of the multivalent vaccine. CONCLUSION: IFN-based drugs enhance the protective effect of vaccination against associative infections in the newborn calves. They stimulate a rise in the titer of antibodies to Rotavirus, coronavirus, VD, and mucosal disease complex as well as an increase in immunoglobulins A, M, and G.