Room Temperature Isothermal Colorimetric Padlock Probe Rolling Circle Amplification for Viral RNA Detection

Seasonal flu and pandemics, which account for millions of infections and hundreds of thousands of deaths, require rapid and reliable detection mechanisms to implement preventive and therapeutic measures. Current detection methods of viral infections have limitations in speed, accuracy, accessibility, and usability. This project presents a novel, widely applicable viral diagnostic test that uses a modified version of rolling circle amplification (RCA) to be sensitive, specific, direct RNA targeted, colorimetric and operable at room temperature. We are specifically detecting the following high-impact viruses: SARS-CoV-2, Influenza A (H1N1pdm09), and Influenza B (Victoria Lineage), although our test can be adapted to any viral infection. Results using synthetic viral DNA and RNA sequences show that our diagnostic test takes approximately one hour, detects femtomolar concentrations of RNA strands, and differentiates between virus strains. We believe implementing our diagnostic test will provide faster responses to future viral-related outbreaks for quicker societal recovery.

Effects of eyes-closed and eyes-open EEG field powers on auditory P300 evoke potentials / 中华行为医学与脑科学杂志

ObjectiveTo investigate the effects of eyes-closed (EC) and eyes-open (EO) EEG field powers on the auditory P300 evoke potentials.MethodsThe EEG was recorded in 12 healthy male college students (20 ～24 years) respectively in their eyes-closed and eyes-open states,and then the auditory P300 paradigm was performed.Results① Alpha band was distributed at the posterior-occipital area,while Alpha-1 (7.5～9.5 Hz) field power were EC as (2.62 ±0.81 ) μV2 and EO as ( 1.80 ±0.56) μV2,with statistical significance (P ＜ 0.01 ) ; Alpha-2 ( 10 ～ 12 Hz) field power were EC as ( 8.55 ± 1.85 ) μV2 and EO as (4.95 ± 1.59 )μV2,also with statistical significance (P＜0.05).②In P300,the Common stimulus elicited clear component N1 (90ms)and P2 (180ms) in the ERPs after stimulus,while there were no significant differences between EC and EO states.Correspondingly,the rare stimulus elicited clear component N2 (207 ms) and P3 (313 ms) in the ERPs after stimulus on-set.Comparing the differences of field potentials of the rare stimulus between the two states,N2field potential in EC ( (0.01 ± 0.71 ) μV) was smaller than that in EO ( ( - 2.13 ± 0.80 ) μV ),with statistical significance (P ＜ 0.05 ) ; while P3 field potential in the EC ( ( 4.16 ± 1.08 ) μV ) was smaller than that in EO ( (4.78 ± 1.20) μV),but without statistical significance (P ＞ 0.05 ).ConclusionThe alpha field power is higher in eyes-closed than in eyes-open state,while performing the same auditory P300 paradigm,significant reduction of field potential in the N2 component are observed in eyes-closed than in eyes-open state.