[The cryopreservation of isolated liver cells]. / Kriokonservirovanie izolirovannykh kletok pecheni.

Recent literature data on cryopreservation of isolated hepatocytes are summarized. The hepatocytes are considered to constitute a promising system for investigation of liver metabolic peculiarities as well as for elucidating mechanisms of low temperature effects on biological systems.

[A non-enzymatic method of preparing isolated hepatocytes: assessment of their morpho-functional activity]. / Nefermentativnyi metod polucheniia izolirovannykh gepatotsitov: otsenka ikh morfo-funktsional'noi aktivnosti.

A method for isolating hepatocytes using the two-step liver perfusion by chelate-containing solutions is described. The hepatocytes obtained are highly inactive for the trypan blue staining test, (not less than 90%), ATP content, the rate of endogenic respiration and its stimulation by succinate, a degree of xenobiotic oxidation.

[Functional characteristics of rat hepatocytes isolated by a nonenzymatic method and incubated in vitro]. / Nekotorye kharakteristiki sostoianiia gepatotsitov krys, izolirovannykh nefermentativnym sposobom i inkubiruemykh in vitro.

A study was made of the effect of normothermic incubation on the metabolic state of parenchymal liver cells, isolated by the nonenzymatic method. In the process of a 2 hour incubation in the nutritious sodium-containing medium, a high level of the energy supply system is maintained, determined by the contents of adenine nucleotides, the rate of endogenous respiration and by its stimulation by succinate.

[Short-term storage of isolated hepatocytes under profound hypothermic conditions]. / Kratkosrochnoe khranenie izolirovannykh gepatotsitov v usloviiakh glubokoi gipotermii.

The hypothermic storage of rat liver parenchymal cells in the sucrose-salt medium is studied. It is demonstrated that short-term hepatocyte storage at 4 degrees C results in a slight imbalance, that evidences for sufficient adaptive potencies of these cells to the temperature fall.

[Cytoplasmic enzyme release into the extracellular medium during the freezing and thawing of leukocytes]. / Vykhod tsitoplazmaticheskikh fermentov vo vnekletochnuiu sredu pri zamorazhivanii--ottaivanii leikotsitov.

The activity of cytoplasmic enzymes (hexokinase, phosphorylase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase) in the extracellular medium following leucocyte freezing-thawing to -30, -70, -140 or -196 degrees C was studied. The appearance of these enzymes in the freezing medium has been found to be associated with the disruption of cellular elements. The source of lactate dehydrogenase activity in the extracellular medium was shown to be due not only to disrupted leucocytes, but also to cells remained after freezing-thawing. Possible mechanisms of the release of enzyme proteins from leucocytes after freezing-thawing are discussed.