Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals.

Studies on host-parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple-enzyme restriction-site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population-genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra-host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.

Trace elements in Ladoga ringed seal (Pusa hispida ladogensis) hair.

Hair samples of two adults, eight molted pups, and four newborn Ladoga ringed seals Pusa hispida ladogensis found stranded ashore on Lake Ladoga, North-West Russia, in 2020-2021, were analyzed for concentrations of trace elements, including toxic heavy metals. Al had the highest median concentration (9.25 µg/g, range 1.26-262 µg/g) among toxic elements, followed by Hg (8.84 µg/g, range 2-12.75 µg/g), and Pb (0.16 µg/g, range 0.04-2 µg/g). The Cd concentration in lanugo compared with adult hair of young-of-the-year was significantly lower, suggesting a barrier effect of the placenta for Cd transport to the fetus. The concentrations of Co, Cr, and Zn were higher before the molt, demonstrating the role of lanugo shedding in the excretion of these metals. The concentrations of Hg, Cd, Pb, and Cu were significantly lower than in the 1990s, and lead and copper continued to decline further after the 2000s. Based on available reference trace element levels in other seal species, we found little evidence of substantially elevated levels or deficiencies in Ladoga seals. Nevertheless, since imbalance of trace elements due to contamination can cause physiological disorders, future trace element monitoring in the Ladoga seal is recommended.

DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals.

Three subspecies of the ringed seal (Pusa hispida) are found in northeastern Europe: P. h. botnica in the Baltic Sea, P. h saimensis in Lake Saimaa in Finland, and P. h. ladogensis in Lake Ladoga in Russia. We investigated the poorly-known cestode helminth communities of these closely related but ecologically divergent subspecies using COI barcode data. Our results show that, while cestodes from the Baltic Sea represent Schistocephalus solidus, all worms from the two lakes are identified as Ligula intestinalis, a species that has previously not been reported from seals. The observed shift in cestode communities appears to be driven by differential availability of intermediate fish host species in marine vs. freshwater environments. Both observed cestode species normally infect fish-eating birds, so further work is required to elucidate the health and conservation implications of cestode infections in European ringed seals, whether L. intestinalis occurs also in marine ringed seals, and whether the species is able to reproduce in seal hosts. In addition, a deep barcode divergence found within S. solidus suggests the presence of cryptic diversity under this species name.