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1.
Biochem Mol Biol Educ ; 46(5): 502-515, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30281891

RESUMO

Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes in a quantitative fashion. Here the contribution of enzymes and transporters to the control of a given pathway flux and metabolite concentrations is determined and expressed quantitatively by means of numerical coefficients. Metabolic flux can be influenced by a wide variety of modulators acting on one or more metabolic steps along the pathway. We describe a laboratory exercise to study metabolic regulation of human erythrocytes (RBCs). Within the framework of MCA, students use these cells to determine the sensitivity of the glycolytic flux to two inhibitors (iodoacetic acid: IA, and iodoacetamide: IAA) known to act on the enzyme glyceraldehyde-3-phosphate-dehydrogenase. Glycolytic flux was estimated by determining the concentration of extracellular lactate, the end product of RBC glycolysis. A low-cost colorimetric assay was implemented, that takes advantage of the straightforward quantification of the absorbance signal from the photographic image of the multi-well plate taken with a standard digital camera. Students estimate flux response coefficients for each inhibitor by fitting an empirical function to the experimental data, followed by analytical derivation of this function. IA and IAA exhibit qualitatively different patterns, which are thoroughly analyzed in terms of the physicochemical properties influencing their action on the target enzyme. IA causes highest glycolytic flux inhibition at lower concentration than IAA. This work illustrates the feasibility of using the MCA approach to study key variables of a simple metabolic system, in the context of an upper level biochemistry course. © 2018 International Union of Biochemistry and Molecular Biology, 46(5):502-515, 2018.


Assuntos
Bioquímica/educação , Eritrócitos/metabolismo , Glicólise , Colorimetria , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Eritrócitos/efeitos dos fármacos , Gliceraldeído-3-Fosfato Desidrogenases/antagonistas & inibidores , Gliceraldeído-3-Fosfato Desidrogenases/metabolismo , Glicólise/efeitos dos fármacos , Humanos , Iodoacetamida/química , Iodoacetamida/farmacologia , Ácido Iodoacético/química , Ácido Iodoacético/farmacologia , Estudantes
2.
PLoS One ; 12(2): e0170607, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28187186

RESUMO

A clear understanding of the structural foundations underlying protein aggregation is an elusive goal of central biomedical importance. A step toward this aim is exemplified by the ß-barrel motif represented by the intestinal fatty acid binding protein (IFABP) and two abridged all-ß sheet forms (Δ98Δ and Δ78Δ). At odds with the established notion that a perturbation of the native fold should necessarily favor a buildup of intermediate forms with an enhanced tendency to aggregate, the intrinsic stability (ΔG°H2O) of these proteins does not bear a straightforward correlation with their trifluoroethanol (TFE)-induced aggregation propensity. In view of this fact, we found it more insightful to delve into the connection between structure and stability under sub-aggregating conditions (10% TFE). In the absence of the co-solvent, the abridged variants display a common native-like region decorated with a disordered C-terminal stretch. Upon TFE addition, an increase in secondary structure content is observed, assimilating them to the parent protein. In this sense, TFE perturbs a common native like region while exerting a global compaction effect. Importantly, in all cases, fatty acid binding function is preserved. Interestingly, energetic as well as structural diversity in aqueous solution evolves into a common conformational ensemble more akin in stability. These facts reconcile apparent paradoxical findings related to stability and rates of aggregation. This scenario likely mimics the accrual of aggregation-prone species in the population, an early critical event for the development of fibrillation.


Assuntos
Proteínas de Ligação a Ácido Graxo/química , Agregados Proteicos , Motivos de Aminoácidos , Animais , Proteínas de Ligação a Ácido Graxo/metabolismo , Estabilidade Proteica , Ratos , Trifluoretanol/química
3.
Biochim Biophys Acta ; 1844(9): 1599-607, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24929115

RESUMO

Δ78Δ is a second generation functional all-ß sheet variant of IFABP (intestinal fatty acid binding protein) corresponding to the fragment 29-106 of the parent protein. This protein and its predecessor, Δ98Δ (segment 29-126 of IFABP), were initially uncovered by controlled proteolysis. Remarkably, although IFABP and Δ98Δ are monomers in solution, Δ78Δ adopts a stable dimeric structure. With the aim of identifying key structural features that modulate the aggregation of ß-proteins, we evaluate here the structure and aggregation propensity of Δ78Δ. The 2,2,2-trifluoroethanol (TFE) induced aggregation of this protein shows a primary nucleation-elongation mechanism, characterized by the stabilization of a dimeric nucleus. Its rate of production from the co-solvent induced aggregation prone state governs the kinetics of polymerization. In this context, the value of Δ78Δ lies in the fact that - being a stable dimeric species - it reduces an otherwise bimolecular reaction to a unimolecular one. Interestingly, even though Δ78Δ and IFABP display similar conformational stability, the abrogated form of IFABP shows an enhanced aggregation rate, revealing the ancillary role played on this process by the free energy of the native proteins. Δ78Δ share with IFABP and Δ98Δ a common putative aggregation-prone central peptide. Differences in the exposure/accessibility of this segment dictated by the environment around this region might underlie the observed variations in the speed of aggregation. Lessons learnt from this natural dimeric protein might shed light on the early conformational events leading to ß-conversion from barrels to amyloid aggregates.


Assuntos
Amiloide/química , Proteínas de Ligação a Ácido Graxo/química , Fragmentos de Peptídeos/química , Sequência de Aminoácidos , Amiloide/ultraestrutura , Floculação , Humanos , Cinética , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Dados de Sequência Molecular , Multimerização Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Termodinâmica , Trifluoretanol/química
4.
Biophys J ; 103(9): 1929-39, 2012 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-23199921

RESUMO

Δ98Δ is a functional all-ß sheet variant of intestinal fatty acid binding protein (IFABP) that was generated by controlled proteolysis. This framework is useful to study the molecular determinants related to aggregation of ß-barrel proteins. Albeit displaying increased conformational plasticity, Δ98Δ exhibits a nativelike ß-barrel topology and is able to support a cooperative folding behavior. Here we present a comparative study of IFABP and Δ98Δ regarding their conformational perturbation and aggregation propensity triggered by trifluoroethanol. Both proteins share a common nucleation-elongation mechanism, whereby the rate-limiting step is the formation of stable dimeric nuclei followed by the association of monomers to the growing aggregates. Despite leading to a less stable structure, the extensive truncation of IFABP yields a form exhibiting a somewhat lower tendency to aggregate. This finding appears at odds with the established notion that a perturbation of the native compact fold should necessarily favor the population of aggregation-prone species. In addition to the aggregation propensity dictated by a given amino-acid sequence, our contention holds that long-range interactions might also play a major role in determining the overall aggregation propensity.


Assuntos
Proteínas de Ligação a Ácido Graxo/química , Dobramento de Proteína , Multimerização Proteica , Deleção de Sequência , Sequência de Aminoácidos , Animais , Dados de Sequência Molecular , Estabilidade Proteica , Estrutura Terciária de Proteína , Subunidades Proteicas , Ratos , Trifluoretanol/farmacologia
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