A Determination of Salivary and Serum Glucose Levels in Patients With Type II Diabetes Mellitus.

AIM: The aim of this work was to determine and compare serum and salivary glucose levels in patients with type II diabetes mellitus (DM). MATERIAL AND METHODS: This study had an overall sample size of 100, which consisted of male and female volunteers aged 30-67 years. Both in-depth interviews and physical tests were conducted. Blood and saliva were collected from the participants while fasting, which were analyzed to determine the levels of salivary alpha-amylase (α-amylase). Both the subjects and the controls were instructed to test their blood glucose levels while fasting and it was suggested that HbA1c values will be used for diagnosing diabetes following the guidelines of the American Diabetes Association, Centers for Disease Control, and World Health Organization. RESULTS: The average age of the control group (Category A) was noted as 47.52±6.28 years, and that of the study group (Category B) was 49.17±7.25 years. In Category A, female (n=23) were 46%, and 54% were male (n=27); and 40% of the people in Category B were female (n=20), and 60% were male (n=30). The majority of patients (54%) in Category B displayed an average level of DM control (n=27), followed by poor control (24%. n=12), well-controlled (20%, n=10), and uncontrolled DM (2%, n=1). Category A had an average salivary α-amylase concentration of 3.1±0.88 U/L, whereas that of Category B was 12.06±2.36 U/L. Thus, the mean salivary α-amylase level of Category B was found to be much higher than that of Category A, and this difference was statistically significant (p<0.001). CONCLUSION: The determination of α-amylase levels in the saliva of individuals suspected of having type II DM has been suggested as a potential diagnostic method. Screenings conducted at healthcare institutions and community health fairs, as well as epidemiological studies, might benefit from this method. We believe that normal clinical practice should include the use of saliva in a broad variety of diagnostic tests.

Comparative evaluation of podoplanin in odontogenic cysts and tumours to determine their proliferative potential-An immunohistochemical study.

Context: Odontogenic cysts and tumours are a wide array of complex pathological entities ranging from mild indolent to aggressive detrimental in nature, which occur as a result of anomalous alterations in normal odontogenesis. Hence, these odontogenic lesions need to be evaluated extensively by using potential immunohistochemical markers. Aim: To evaluate and compare the expression of podoplanin, a lymphoendothelial IHC marker in odontogenic cysts and odontogenic tumours to determine their proliferative potential. Settings and Design: All the study samples were retrieved from the archives of the Department of Oral Pathology and Microbiology, PIDS&RC, Hyderabad. The study samples were selected as per the standard histopathological diagnostic criteria and subjected for IHC analysis using podoplanin. Method and Materials: Seventy paraffin-embedded tissue specimens of OKC, OOC, dentigerous cyst (DC) and ameloblastoma (AM) include study sample, which were stained with podoplanin IHC marker and staining properties were evaluated. All the cases were categorized as high, moderate, weak or negatively reactive on the basis of the composite scoring. Statistical Analysis Used: Statistical analysis was done using SPSS version 14, and then results were compared by ANOVA post hoc test and Kruskal Wallis Test. Results: In the comparison of composite scores of OKCs and AM, there was no significant statistical difference. Conclusion: The present study contributes to the significant association of podoplanin expression with cellular proliferation, cystic expansion and local invasiveness of odontogenic cysts and tumours through cytoskeletal reorganization and cell migration.

To Evaluate the Level of Awareness of Patients About Existing Oral Precancerous Lesions: A Longitudinal Study.

Background: The prevalence of oral cancer and other oral premalignant diseases (OPMDs) is increasing. Particularly among lower socioeconomic countries, awareness with respect to oral cancer is quite poor. The aim of the current study is to evaluate the level of awareness of patients about existing oral precancerous lesions. Materials and Methods: Fifty people with existing oral precancerous lesions whose diagnosis was confirmed by a clinician's examination were included in this research. Questions were asked of patients to fill out a questionnaire; those with cancer, non-precancerous red or white lesions, or no lesions in the mouth were not included. Questions on demographics, precancer knowledge, precancer information sources, and tobacco/betel chewing, smoking, and alcohol use were included in the survey. Informed by prior research and knowledge of precancerous lesions and circumstances, a closed-ended questionnaire was developed. Results: The study found that just 44 percent of participants were aware that they had oral lesions or diseases. Self-examination was the most common method (81.82%), followed by inquiring with friends and family (9.09%) and medical professionals (9%). Most patients (41.67 percent) learn about oral precancers via the media, followed by posters and banners (33.33%), friends and family (12.5%), other sources (8.33%), and physicians (4.17%). About half of the patients with oral precancers chewed tobacco for 11 years on average, doing so 4-5 times a day. Another 38% smoked and drank alcohol, also at 4-5 times a day. Oral lichen planus (16%), Oral Submucous fibrosis (12%), and Erythroplakia (12%) were shown to be the next most common oral lesions after tobacco pouch keratosis (30%) and leukoplakia (30%). Conclusion: Research indicates that few people are aware of the existence of oral precancerous lesions or diseases. Patients' awareness of oral precancerous lesions/conditions was shown to be significantly influenced by media such as television, radio, posters, and banners.

Evaluation of Hematological Profile in Oral Submucous Fibrosis.

BACKGROUND: The aim of the study is to assess the hematological profile in oral submucous fibrosis (OSMF) patients. METHODS: The study's participants (100) were divided into two groups. Group I consisted of fifty areca nut chewers with complaints of burning sensations, blanching, and stiffness of the oral mucosa. Group II consisted of fifty healthy patients as controls. A hematological profile was estimated in all subjects. RESULTS: The control group had a mean hemoglobin (Hb) of 13.87±1.26 g/dL, while the OSMF group had a mean Hb of 11.03±2.16 g/dL (P=0.001). The mean serum iron level in the control group was 120.36±41.22 g/dL, while it was 44.97±13.45 g/dL in the OSMF group (P=0.001). The control group's mean serum vitamin B12 values were 424.77±110.95 g/dL, while the OSMF group's was 210.11±44.88 g/dL (P=0.001). In the research population, 47 patients had iron deficiency. The odds ratio (OR) was 28.11, which meant that the high prevalence of iron deficiency was predicted 28.11 times more often than in the control group. CONCLUSION: As part of a biochemical assessment, iron status is assessed as part of a prevention mechanism for people who are at high risk. Biochemical testing has been suggested as a potential tool for mass screening OSMF patients.

Oral Manifestations in HIV-TB Co- infected Patients and Their Correlation with CD4 Count in Telangana State, India.

AIMS AND OBJECTIVES: Human immunodeficiency virus (HIV)-related oral lesions are often an early finding, and they reflect the underlying immunosuppression, and tuberculosis (TB) coinfection can have further deteriorating effect. Hence, a cross-sectional study was conducted to evaluate clinical and oral presentations of patients coinfected with HIV-TB, correlating with various parameters such as the type of TB with CD4 cell count, the type of TB with oral manifestations, site of the lesion, oral manifestations with CD4 cell counts, age, and gender. MATERIALS AND METHODS: A cross-sectional study was conducted among selected 200 patients coinfected with HIV-TB, registered at Gandhi Medical College, Hyderabad, Telangana, India, and demographic data, CD4 count, diagnosis of TB, and clinical presentation of TB were correlated with site, age, gender, and the type of lesions in the oral cavity. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software, (IBM SPSS), version 20 (Chicago, IL, USA), with the chi-square test, and the significant P value for all the parameters was considered as <0.05. RESULTS: A total of 200 patients with HIV-TB coinfection, who presented with oral lesions of 258 coinfected cases, were examined. Among which, 129 patients were with pulmonary tuberculosis (PTB), 61 patients with extrapulmonary TB, 2 patients with disseminated TB, and 8 patients with PTB and pneumonia. There were multiple oral manifestations involving different sites of oral cavity, oral candidiasis (28.5%), angular cheilitis (24.5%), linear gingival erythema (21.5%), oral hairy leukoplakia (1.5%), melanotic pigmentation (29.0%), ulcers (20.0%), depapillation of tongue (26.5%), lobulated tongue (12.0%), hairy tongue (11.5%), and papules (10.0%). The correlation of the type of TB with CD4 cell count, oral lesions with the type of TB in tongue, labial mucosa, and palate was significant. CONCLUSION: A total of 77.5% patients coinfected with HIV-TB had shown oral manifestations emphasizing that the presence of oral lesions can be considered as a strong indicator of coinfection. The oral lesions might be used as a clinical indicator or screening mechanism in patients who were HIV seropositive for TB coinfection and should be necessarily evaluated for TB.