Ivermectin resistance in the multi-host tick Hyalomma anatolicum (Acari: Ixodidae) in India.

The multi-host tick, Hyalomma anatolicum is a widely distributed vector of many pathogens of veterinary and public health importance. Ivermectin (IVM), as an alternative to control pyrethroid-resistant ticks, has been used extensively for the past 4-6 years in tropical and sub-tropical countries including India resulting in declining tick control efficacy. The present study used adult immersion test (AIT) to examine the resistance status of H. anatolicum collected from three districts in the Indian state of Gujarat against ivermectin. Probit analysis was used for calculation of concentration-mortality regressions; concentrations required for 50% mortality (LC50) and 95% mortality (LC95), along with confidence intervals; slope of mortality; % inhibition of oviposition; and discriminating concentration (DC). The calculated LC50 and LC95 estimates were utilized to determine resistance ratios (RR50, RR95) and the resistance levels (RL) of the field ticks compared to the susceptible population. The DC (2 x LC95) for IVM was calculated as 84.48 ppm, using susceptible H. anatolicum ticks (KHD). Lower estimates of the coefficient of non-determination (1-R2) for AIT ranged from 0.06 to 0.27, and the range of RR50 and RR95 values against IVM was estimated to be from 1.43 to 52.06 and 1.14 to 71.99, respectively, which indicated a varying degree of resistance among the field tick populations. Based on RR50 values, tick populations from Danta and Palanpur showed resistance level IV and II, respectively. Another four populations (Vadgam, Kankrej, Saraswati and Sidhpur) were classified as having level I resistance status against IVM. To our knowledge, this is the first report of ivermectin resistance in H. anatolicum from Gujarat, India.

Test day variability in yield and composition of Surti and Mehsani buffaloes milk at day 15 and 60 postpartum.

AIM: To estimate individual test day variability in yield and composition of Surti and Mehsani buffaloes milk at day 15 and 60 postpartum (pp). MATERIALS AND METHODS: A total of 13 normally calved Surti and Mehsani buffaloes each maintained at Livestock Research Stations of Navsari and Sardarkrushinagar Dantiwada Agricultural Universities, respectively, were selected for the study. Milk sample was collected from each selected buffalo at day 15 and 60 pp to study milk yield and composition variability between these two breeds. Buffaloes were categorized for the ease of data analysis and comparisons into four groups, viz., S15 (Surti buffaloes 15(th) day pp), S60 (Surti buffaloes 60(th) day pp), M15 (Mehsani buffaloes 15(th) day pp), and M60 (Mehsani buffaloes 60(th) day pp). RESULTS: There were 37.20% and 25.03% significant (p≤0.05) increase in mean test day milk yield (TDMY) of S60 and M60 as compared to S15 and M15 groups, respectively. The mean TDMY of Mehsani buffalo was 99.19% and 81.53% significantly (p≤0.05) higher than Surti buffaloes at day 15 and 60 pp, respectively. The mean fat and protein corrected test day milk yield (FPCTDMY) of all the groups was found to be significantly different (p≤0.05) from each other. There was significant (p≤0.05) increase of 1.94 and 3.45 kg in mean FPCTDMY with the progression of lactation between day 15 and 60 pp in Surti and Mehsani buffaloes, respectively. Similarly, the mean FPCTDMY of Mehsani buffaloes were approximately double with 103.27% and 96.36% higher yield as compared to Surti buffaloes at day 15 and 60 pp, respectively. Among milk composition, significant differences were observed for solid not fat (SNF) and protein%, whereas fat and lactose% were steady among four groups. The only significant (p≤0.05) difference was observed for SNF in M60 group, which was 8.29%, 6.85%, and 10.70% higher as compared to S15, S60, and M15 groups, respectively. The mean protein% in milk of Mehsani buffaloes was 21.01% and 33.05% significantly (p≤0.05) higher than Surti buffaloes milk at day 15 and 60 pp, respectively. However, there was a significant difference in protein% observed with the advancement of lactation in Mehsani buffaloes, but it was not so in the case of Surti buffaloes. CONCLUSION: Major consistent finding of the present study reveals that milk yield and protein% of Mehsani buffalo was significantly higher than Surti buffalo at day 15 and 60 pp.

Genetic analysis of first lactation production traits in Kankrej cattle.

AIM: The aim was to estimate genetic factors affecting the first lactation milk production traits in Kankrej cattle of North Gujarat. MATERIALS AND METHODS: The 475 first lactation records of Kankrej cows that were maintained at the Livestock Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, over a period of 35 years from 1980 to 2014 were studied. The least squares maximum likelihood program was used to estimate genetic parameters of first lactation traits. Heritability was estimated through paternal half-sib analysis in adjusted data. RESULTS: The heritability estimate for production traits was 0.40±0.17, 0.45±0.17, 0.35±0.18, and 0.20±0.14 for standard 300 days milk yield (F300Y), total lactation milk yield (FLY), wet average (FWA), and lactation length (FLL), respectively, in the first parity. All the genetic and phenotypic correlations among different production efficiency traits were high and positive. Genetic correlations between F300Y and FLY, FLL, and FWA were 0.80±0.20, 0.59±0.16, and 0.81±0.32, where as the phenotypic correlations were 0.969, 0.688, and 0.868, respectively. Genetic correlations of FLY with FLL and FWA were 0.60±0.13 and 0.79±0.20, whereas the phenotypic correlations were 0.777 and 0.817, respectively. Genetic and phenotypic correlation between FLL and FWA was 0.63±0.28 and 0.31, respectively. CONCLUSION: The heritability estimate of all first parity lactation traits waslow to medium (0.20-0.45) indicated the scope for further improvement in this trait through selection as well as managemental practice. Higher genetic and phenotypic correlation between thefirst lactation milk production traits gives theidea that genetic gain due to selection for one trait also givesmorecorrelated response of selection for other traits which is economically advantageous.