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1.
Neotrop Entomol ; 46(6): 642-648, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28255952

RESUMO

In the present work, we explored multiple data from different biological levels such as cuticular hydrocarbons, chromosomal features, and mtDNA sequences in the Neotropical social wasp Mischocyttarus consimilis (J.F. Zikán). Particularly, we explored the genetic and chemical differentiation level within and between populations of this insect. Our dataset revealed shallow intraspecific differentiation in M. consimilis. The similarity among the analyzed samples can probably be due to the geographical proximity where the colonies were sampled, and we argue that Paraná River did not contribute effectively as a historical barrier to this wasp.


Assuntos
Cromossomos de Insetos , DNA Mitocondrial/genética , Hidrocarbonetos/análise , Vespas/genética , Vespas/metabolismo , Estruturas Animais/metabolismo , Animais , Vespas/química
2.
Genet Mol Res ; 7(2): 559-66, 2008 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-18752181

RESUMO

We have been able to discriminate different castes and sexes of ants in the same colony by measuring cuticular hydrocarbon levels with Fourier transform infrared photoacoustic spectroscopy, compared by canonical discriminant function analysis. We have now applied this methodology to various colonies of two species of ants of the genus Ectatomma in the Brazilian Cerrado. There were clear interspecific differences in cuticular hydrocarbons of these ants, with a small intraspecific variation. The differences between colonies were greater in E. brunneum than in E. vizottoi. Genetic differences among the colonies and species were well estimated by Fourier transform infrared photoacoustic spectroscopy and statistical analyses.


Assuntos
Formigas/química , Hidrocarbonetos/análise , Proteínas de Insetos/química , Animais , Brasil , Proteínas de Insetos/análise , Especificidade da Espécie , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
3.
Genet Mol Res ; 6(3): 492-9, 2007 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-17985301

RESUMO

Fourier transform-infrared photoacoustic spectroscopy was applied for the first time, to our knowledge, to distinguish different castes of an ant species. The method was applied directly to the abdomen of queens, workers and males of Ectatomma vizottoi ants, without any special sample preparation. The absorption bands of secondary amide and hydrocarbons were identified; using these as variables in a canonical discriminant analysis we found significant differences between the castes. Queens have a greater hydrocarbon content than do workers and males, which is related to their function in the colony. This technique can be used to analyze and distinguish small chemical differences in biological systems, even in opaque samples.


Assuntos
Espectrofotometria Infravermelho/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Animais , Formigas , Comportamento Animal , Feminino , Hidrocarbonetos/química , Hidrocarbonetos/metabolismo , Masculino , Análise Multivariada , Feromônios/metabolismo , Comportamento Social , Espectrofotometria/métodos
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