Asynchronous Adaptive Threshold Level Crossing ADC for Wearable ECG Sensors.

The level crossing ADC generates digitized samples consisting of the magnitude of input signal and time interval between two consecutive level crossings when the input signal crosses the threshold level. This paper presents a new architecture of low power asynchronous adaptive threshold level crossing (LC) ADC suitable for wearable ECG sensors based on a novel algorithm for determining adaptive threshold. The adaptive threshold was determined by calculating the mean of maximum and minimum values of signal in a predetermined window. Polynomial interpolation was used to reconstruct the signal. A signal to noise distortion ratio of 57.50 dB and a mean square error (MSE) measure of 1.368*10-8 V2 was achieved by the proposed algorithm for a 1 mV, 10 Hz input sinusoidal signal in MATLAB. The asynchronous adaptive threshold LC ADC operating from a supply voltage of 0.8 V occupied a layout area of 266.33*331.385 µm2 when implemented in CADENCE virtuoso using 180 nm technology. The designed circuit consumes an average power of 367.6 nW for a 1mVpp, 10 Hz input sinusoidal signal when simulated in Virtuoso.

Mechanism of drug resistance in a clinical isolate of Vibrio fluvialis: involvement of multiple plasmids and integrons.

The role of mobile genetic elements in imparting multiple drug resistance to a clinical isolate of Vibrio fluvialis (BD146) was investigated. This isolate showed complete or intermediate resistance to all of the 14 antibiotics tested. Polymerase chain reaction (PCR) revealed the presence of a class 1 integron and the absence of the SXT element in this isolate. The strain harboured a 7.5 kb plasmid and a very low copy number plasmid of unknown molecular size. Transformation of Escherichia coli with plasmid(s) from BD146 generated two kinds of transformants, one that harboured both of these plasmids and the other that harboured only the low copy number plasmid. PCR and antibiogram analysis indicated the association of the class 1 integron with the low copy number plasmid, which also conferred all the transferable resistance traits except trimethoprim to the parent strain. A BLAST search with the sequence of the 7.5kb plasmid showed that it was 99% identical to plasmid pVN84 from Vibrio cholerae O1 in Vietnam, indicating that these two plasmids are probably one and the same. To the best of our knowledge, this is the first report of horizontal transfer of a plasmid between V. fluvialis and V. cholerae.