RESUMO
The migration of metal ions to the food matrix has been always a challenge in the production of active food packaging films. In this study, it was tried to evaluate the idea of using hairy cellulose nanocrystals (HCNs) in controlling the migration of Silver Nanoparticles (AgNPs) from polycaprolactone (PCL)-based films to the Tilapia fish. HCNs and the final films (integrated with various amounts of HCNs and AgNPs) were evaluated physicochemically and mechanically. Tilapia fish were packed using the films and after specific periods, the fish samples were assessed microbiologically and physiochemically. According to the results, incorporating NPs into PCL films enhanced tensile strength, elasticity, and toughness making the films more resistant to breakage and deformation under stress. The introduction of HCNs reduced the surface roughness level, decreasing AgNPs migration, but also accelerated the degradation rate. Films with [1% AgNPs +2% HCNs] and [1% AgNPs] had the lowest and highest water vapor transmission rate. The use of AgNPs (1%) + HCNs (2%) incorporated into PCL films resulted in a lower pH value, TVB-N, TBARs, and PV. It also decreased microbial activities in samples in comparison to the control. Therefore, the idea of using HCNs along with antibacterial metal-based nanoparticles can control the rate of ion migration.
RESUMO
Biosynthesis of nanoparticles (NPs) using plant extract is an eco-friendly method, in which natural materials are used and is a simple, non-toxic, and environmentally friendly green synthesis. In this study, corn starch (CS) film containingSatureja khuzestanicaessential oil (SEO) and Ag-TiO2nanocomposites (size: nearly 30-60 nm) were prepared and its antimicrobial, morphological, physical, and mechanical characteristics were investigated. Ag-TiO2nanocomposites with different molar percentages were synthesized byS. khuzestanicaextract and based on the best antibacterial results against Gram-negative bacteria (Escherichia coliATCC 25922 andSalmonella typhimuriumATCC 14028) and Gram-positive bacteria (Staphylococcus aureusATCC 25923), were chosen to prepare the films. Four types of biodegradable films were provided: simple CS film, the film incorporated with SEO (essence film), the film incorporated with Ag-TiO2nanocomposites (nanofilm), and nano/essence film. The scanning electron microscopy (SEM) was employed for investigating the morphology of the films. The combined energy-dispersive x-ray spectroscopy with SEM was applied to analyze the near-surface elements. Physical characteristics of the films containing water vapor permeability (%) and their moisture content, mechanical tests, and antibacterial properties were examined. Antimicrobial evaluation of the films revealed a 3-4 log and 6-7 log (CFU ml-1) reduction inS. aureusandE. colispecies respectively, compared to the control group. The bio-polymer film incorporated with extracted essential oil ofS. khuzestanicaand Ag-TiO2nanocomposites are effective to package foods and can delay chemical, physical, and microbial spoilage.
