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1.
Biosci Biotechnol Biochem ; 78(11): 1954-62, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25351334

RESUMO

Low protein content and sufficient grain rigidity are desired properties for the rice used in high-quality sake brewing such as Daiginjo-shu (polishing ratio of the rice, less than 50%). Two kinds of rice, sake rice (SR) and cooking rice (CR), have been used for sake brewing. Compared with those of SR, analyses of CR for high-quality sake brewing using highly polished rice have been limited. Here we described the original screening of late-maturing CR Sensyuraku (SEN) as rice with low protein content and characterization of its properties for high-quality sake brewing. The protein content of SEN was lower than those of SR Gohyakumangoku (GOM) and CR Yukinosei (YUK), and its grain rigidity was higher than that of GOM. The excellent properties of SEN with respect to both water-adsorption and enzyme digestibility were confirmed using a Rapid Visco Analyzer (RVA). Further, we confirmed a clear taste of sake produced from SEN by sensory evaluation. Thus, SEN has excellent properties, equivalent to those of SR, for high-quality sake brewing.


Assuntos
Bebidas Alcoólicas , Fermentação , Oryza/química , Oryza/metabolismo , Culinária
2.
Biosci Biotechnol Biochem ; 77(12): 2505-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24317072

RESUMO

In the fermentation industry, the traceability of microorganisms during the process is important to ensure safety and efficacy. Ethyl carbamate, a group-2A carcinogen, is produced from ethanol and urea during the storage of food/alcoholic beverages. We isolated non-urea-producing sake yeast car1 mutants carrying a discriminable molecular marker, and demonstrated, by the use of PCR assays, that these mutants are useful for traceability analysis and identification during the sake brewing process.


Assuntos
Bebidas Alcoólicas/microbiologia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/isolamento & purificação , Arginase/genética , Biomarcadores/metabolismo , Fermentação , Loci Gênicos/genética , Mutação , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/metabolismo
3.
Biosci Biotechnol Biochem ; 77(10): 2160-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24096657

RESUMO

The Japanese high-quality sake Daiginjo-shu is made from highly polished rice (polishing ratio, less than 50%). Here we showed that the sake rice Koshitanrei (KOS) has an excellent polishing property. Rice grains of KOS had the same lined white-core region as the sake rice Yamadanishiki (YAM). The grain rigidity/hardness of KOS was higher than that of the sake rice Gohyakumangoku (GOM). The loss ratio of KOS after high polishing by an industrial polishing machine was lower than that of GOM. Further, a clear taste of sake produced from KOS was confirmed by sensory evaluation.


Assuntos
Bebidas Alcoólicas/normas , Manipulação de Alimentos/métodos , Oryza , Fenômenos Mecânicos , Oryza/classificação , Fatores de Tempo
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