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1.
J Anim Sci ; 93(5): 2154-64, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26020311

RESUMO

Pigs fed protein-bound AA appear to have a higher abundance of AA transporters for their absorption in the jejunum compared with the duodenum. However, there is limited data about the effect of dietary free AA, readily available in the duodenum, on the duodenal abundance of AA transporters and its impact on pig performance. Forty-eight pigs (24.3 kg initial BW) distributed in 4 treatments were used to evaluate the effect of the CP level and form (free vs. protein bound) in which AA are added to diets on the expression of AA transporters in the 3 small intestine segments, serum concentration of AA, and performance. Dietary treatments based on wheat and soybean meal (SBM) were 1) low-CP (14%) diet supplemented with L-Lys, L-Thr, DL-Met, L-Leu, L-Ile, L-Val, L-His, L-Trp, and L-Phe (LPAA); 2) as in the LPAA but with added L-Gly as a N source (LPAA+N); 3) intermediate CP content (16%) supplemented with L-Lys HCl, L-Thr, and DL-Met (MPAA); and 4) high-CP (22%) diet (HP) without free AA. At the end of the experiment, 8 pigs from LPAA and HP were sacrificed to collect intestinal mucosa and blood samples and to dissect the carcasses. There were no differences in ADG, ADFI, G:F, and weights of carcass components and some visceral organs between treatments. Weights of the large intestine and kidney were higher in HP pigs (P < 0.01). Expression of b(0,+) in the duodenum was higher in pigs fed the LPAA compared with the HP diet (P= 0.036) but there was no difference in the jejunum and ileum. In the ileum, y+ L expression tended to be higher in pigs fed the LPAA diet (P = 0.098). Expression of b(0,+) in LPAA pigs did not differ between the duodenum and the jejunum, but in HP pigs, the expression of all AA transporters was higher in the jejunum than in the duodenum or ileum (P < 0.05). The serum concentration of Arg, His, Ile, Leu, Phe, and Val was higher but serum Lys and Met were lower in pigs fed the HP diet (P < 0.05). These results indicate that LPAA can substitute up to 8 percentage units of protein in HP wheat-SBM diets without affecting pig performance; nonessential N does not seem to be limiting in very low-protein wheat-SBM diets for growing pigs. Also, the inclusion of free AA in the diet appears to affect their serum concentration and the expression of the AA transporter b0,+ in the duodenum of pigs.


Assuntos
Sistemas de Transporte de Aminoácidos/metabolismo , Dieta com Restrição de Proteínas/veterinária , Suplementos Nutricionais , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Sus scrofa/crescimento & desenvolvimento , Sus scrofa/metabolismo , Aminoácidos/metabolismo , Ração Animal/análise , Animais , Duodeno/metabolismo , Íleo/metabolismo , Mucosa Intestinal/metabolismo , Jejuno/metabolismo , Glycine max , Suínos , Triticum
2.
J Anim Physiol Anim Nutr (Berl) ; 99(4): 701-9, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25354230

RESUMO

Lysine (Lys) is the first limiting amino acid (AA) in most feed formulations for pigs and most abundant, along with leucine, in muscle proteins. An experiment was conducted with 17 pigs (17.7 ± 0.05 kg initial BW) to identify a role of dietary Lys in the control of protein synthesis in pigs. Fourteen pigs were randomly assigned to one of the two wheat-based dietary treatments: Lys-deficient, 3.0 g/kg (DEF) and Lys-adequate, 10.8 g/kg (ADE). Samples from jejunum mucosa, liver, Longissumus and Semitendinosus muscles, and blood were collected. The other three pigs were sacrificed at the beginning of the trial to measure basal carcass composition. Weight gain, gain:feed ratio, Lys intake and loin eye area were greater in ADE than in DEF pigs (p < 0.01). Muscle-related carcass characteristics were better, and myosin heavy chain IIb expression (MyHC IIb) in Semitendinosus was higher in ADE than in DEF pigs. Expression of AA transporters CAT-1 was lower (p < 0.05), serum Lys was higher and serum Val was lower in pigs fed the ADE diet. The higher muscularity, MyHC IIb expression in Semitendinosus muscle and Lys serum of pigs fed the ADE diet suggest that Lys increases growth rate not only by functioning as protein construction unit but also as potential control of the protein synthesis process.


Assuntos
Aminoácidos/sangue , Regulação da Expressão Gênica/efeitos dos fármacos , Lisina/farmacologia , Músculo Esquelético/crescimento & desenvolvimento , Suínos/crescimento & desenvolvimento , Aumento de Peso/efeitos dos fármacos , Sistemas de Transporte de Aminoácidos/genética , Sistemas de Transporte de Aminoácidos/metabolismo , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Suplementos Nutricionais , Lisina/administração & dosagem , Dados de Sequência Molecular
3.
J Anim Physiol Anim Nutr (Berl) ; 95(2): 179-86, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20666859

RESUMO

The effects of phytase supplementation on the apparent ileal digestibility (AID) of amino acids (AA) have been inconsistent. Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin(®) ) to growing pigs fed sorghum-soybean meal diets supplemented with phytase on the AID of AA, energy, and phosphorus (P), as well as the ileal digestibility (ID) of phytate; there were four periods per experiment. In Experiment 1, eight pigs (BW 22.1±1.3 kg) were fitted with a T-cannula at the distal ileum. Each period consisted of 9 days; 7 days for diet adaptation, and 2 days for digesta collection. Treatments (T) were: (i) basal sorghum-soybean meal diet, (ii) basal diet plus Pancreatin®, (iii) basal diet plus phytase and (iv) basal diet plus phytase and Pancreatin®. Phytase increased the digestibilities of phytate and P (p<0.001), but did not affect the AID of AA and energy (p>0.10). Except for methionine (p=0.07), Pancreatin® did not affect the AID of AA. Phytase and Pancreatin® did not interact (p>0.10). Experiment 2 was similar to Experiment 1, but Pancreatin® was infused into duodenum. Pancreatin® infusion did not affect the AID of AA (p>0.10); and tended to reduce (p=0.09) the AID of lysine. Phytase × Pancreatin® interactions were not observed (p>0.10). In conclusion, phytase and Pancreatin® did not improve the AID of AA in growing pigs fed sorghum-soybean meal diets indicating that phytates did not affect AA digestibility.


Assuntos
Aminoácidos/metabolismo , Digestão/efeitos dos fármacos , Íleo/fisiologia , Fósforo/metabolismo , Ácido Fítico/metabolismo , Suínos/metabolismo , 6-Fitase/farmacologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Suplementos Nutricionais , Pancreatina/farmacologia , Sorghum/química , Sorghum/metabolismo
4.
J Anim Sci ; 85(12): 3303-12, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17785591

RESUMO

Diarrhea incidence in weaned pigs may be associated with the concentration of intestinal microbial metabolites (ammonia, amines, and VFA) that are influenced by dietary CP content. Three experiments were conducted to determine effects of a low-protein, AA-supplemented diet on ileal AA digestibility, growth performance, diarrhea incidence, and concentration of microbial metabolites in ileal and cecal digesta of pigs weaned at 14 d of age. In Exp. 1, 8 pigs fitted with a simple T-cannula at the distal ileum were assigned in a crossover design to 2 diets containing 24 or 20% CP using wheat, corn, full-fat soybeans, whey powder, fish meal, and blood plasma as the main ingredients. Supplemental AA were added to the diets to meet the AA standards according to the 1998 NRC recommendations. Chromic oxide was used as an indigestible marker. Diets were fed at 2.5 times the ME requirement for maintenance. The reduction of dietary CP decreased (P < 0.05) the apparent ileal digestibility of most AA, except Lys, Met, Thr, Val, and Pro. Dietary CP content did not affect the pH of ileal digesta or ileal concentrations of ammonia N, cadaverine, putrescine, or VFA. In Exp. 2, 8 pigs fitted with a simple T-cannula in the cecum were assigned to 2 diets, similar to Exp. 1. Dietary CP content did not affect the pH of cecal digesta. The reduction in CP content decreased (P < 0.05) cecal ammonia N, acetic acid, isobutyric acid, isovaleric acid, total VFA, and putrescine concentrations by 28 to 39%. In Exp. 3, 32 pigs were assigned to 2 diets, similar to Exp. 1, according to a randomized complete block design. Pigs had free access to feed and water. Dietary CP content did not affect growth performance or fecal consistency scores during the 3-wk study, and diarrhea was not observed. The results of these experiments indicate that lowering the dietary CP content combined with supplementation of AA markedly reduced the production of potentially harmful microbial metabolites in cecal digesta of early-weaned pigs without affecting growth performance.


Assuntos
Aminoácidos/metabolismo , Proteínas Alimentares/farmacologia , Digestão , Suínos/crescimento & desenvolvimento , Suínos/metabolismo , Aminoácidos/análise , Amônia/análise , Amônia/metabolismo , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Animais Recém-Nascidos , Ceco/metabolismo , Ceco/microbiologia , Estudos Cross-Over , Diarreia/epidemiologia , Diarreia/veterinária , Relação Dose-Resposta a Droga , Ácidos Graxos Voláteis/biossíntese , Conteúdo Gastrointestinal/química , Concentração de Íons de Hidrogênio , Íleo/metabolismo , Íleo/microbiologia , Masculino , Distribuição Aleatória , Doenças dos Suínos/epidemiologia , Desmame
5.
J Anim Sci ; 85(3): 700-5, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17121975

RESUMO

An experiment was conducted with growing pigs to determine the excretion of P and N in 4 barley-based diets formulated to contain 18 or 15% CP by using a normal barley (NB) or a low-phytate barley (LPB). The NB contained 0.31% total P and 0.19% phytate P; the LPB contained 0.32% total P and 0.01% phytate P. The diets were supplemented, when so required, with lysine, methionine, threonine, and tryptophan to meet their apparent ileal digestible supplies according to the NRC (1998). The diets containing NB were supplemented with inorganic P to meet the NRC (1998) recommendation for available P (0.23%). The diets containing LPB were not supplemented with inorganic P because these contained sufficient available P (0.27%). Eight barrows with an average BW of 20.9 kg were assigned to the 4 dietary treatments according to a repeated 4 x 4 Latin square design. The diets were fed at a rate of 2.5 times the ME requirement for maintenance. The barrows were fed twice daily, at 0800 and 1500, equal amounts each meal. Water was added to the feed at a ratio of 2.5:1. Each experimental period consisted of a 7-d adaptation period followed by a 5-d collection of feces and urine. The substitution of NB with LPB decreased (P < 0.001) the total P excretion by 38 and 43% for the 18 and 15% CP diets, respectively. Reducing the CP content from 18 to 15% decreased (P < 0.001) the N excretion by 29 and 32% for the NB and LPB diets, respectively. With the reduction in CP content, there was a decrease (P < 0.001) in the amount of N retained. The N:P ratio in manure of pigs fed the LPB diets was greater (P < 0.001) than from pigs fed the NB diets. These data indicate that P and N excretion can be greatly reduced by substitution of NB by LPB, and also by the reduction of the CP content, in diets for growing pigs.


Assuntos
Proteínas Alimentares/farmacologia , Glycine max/metabolismo , Hordeum/metabolismo , Nitrogênio/metabolismo , Fósforo/metabolismo , Ácido Fítico/análise , Suínos/crescimento & desenvolvimento , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Fezes/química , Masculino , Nitrogênio/análise , Nitrogênio/urina , Fósforo/análise , Fósforo/urina , Ácido Fítico/farmacologia
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