Imunidade celular em camundongos inoculados com fraçöes da parede celular de Paracoccidioides brasiliensis / Cellular immunity in mouse inoculated with fractions from the cell wall of Paracoccidioides brasiliensis

A parede celular do fungo Paracoccidioides brasiliensis (PbHC), em sua forma leveduriforme, foi segmentada em três fraçöes, 1, 2 e 3, que inoculadas em camundongos, foram avaliadas quanto à capacidade de induzir resposta imunecelular. O valor médio da espessura da pata foi semelhante para as três fraçöes, com manutençäo da resposta entre 24 e 48 horas no desafio com a fraçäo 1

Incidência das freqüências gênicas dos sistemas ABO e Rh (incluindo variante D(u)) em populaçäo da cidade de Araraquara, SP / Incidence of the gene frequencies of the ABO and Rh (including the D(u) variant) systems in population of Araraquara, SP

Avaliou-se a distribuiçäo dos sistemas ABO e Rh (inclusive D(u)) em 14.037 indivíduos da cidade de Araraquara, Estado de Säo Paulo. Freqüências fenotípicas e genotípicas foram estimadas para esses indivíduos. A populaçäo do estado de Säo Paulo, representada por sete cidades de diferentes regiöes, foi tomada como parâmetro de comparaçäo. Os resultados evidenciaram para o sistema ABO comparaçöes significativamente diferentes entre esse estudo e os das demais cidades, com exceçäo de Santo André e um dos estudos da cidade de Säo Paulo. Com relaçäo ao Rh observaram-se diferenças significativas entre Araraquara e as cidades de Botucatu (uma das amostragens), Säo José dos Campos e Säo Paulo, excetuando-se Campinas, Itapetininga, Santo André, Säo Carlos e a outra amostra de Botucatu. A freqüência gênica do fator D(u) encontra-se dentro de limites mínimos e máximos em relaçäo aos outros estudos efetuados no estado de Säo Paulo

Presenca de inibidor específico de fixaçào de complemento : Em antígenos preparados de trypanosoma cruzi / Presence of specific complement-fixation inhibitor in antigens prepared from trypanosoma cruzi

Tritation performed by the quantitative complement fixation method on sera from parasitologically confirmed Chagas' disease eases using an aqueous of a methylic extract of Tripanosoma cruzi as antigen, revealed a marked discrepancy in titers. The serum titer was expressed by the slope of the regresion line between amounts of serum and number of complement units needed for 50 per cent hemolysis

In every case, the highest titers were obtained with the methylic antigen. It was observed also that the aqueous antigen, when added to the methylic antigen, produced a significant drop in titer. Everything suggested the presence of an hapten, able to combine specifically with Chagasic antibodies to form immune complexes which do not fix complement

Since the inhibiter was soluble in water and almost insoluble in methanol, it could be isolated as an aqueous extract from trypanosomes previously treated by methanol. This hapten gave very weak complement fixation reactions with Chagasic sera. However, when added to the methylic antigen, a strong inhibition was observed. This inhibition was observed only with Chagasic sera, and not with sera from cases of syphilis, brucellosis, leprosy or schistosomiasis

An antigen free of inhibiter can be prepared by treating the dried trypanosomes, previously delipidized by benezen, with water saturated with chloroform. The extract trypanosomes are dried again and ...(AU)

Presenca de inibidor específico de fixação de complemento : Em antígenos preparados de trypanosoma cruzi / Presence of specific complement-fixation inhibitor in antigens prepared from trypanosoma cruzi

Tritation performed by the quantitative complement fixation method on sera from parasitologically confirmed Chagas' disease eases using an aqueous of a methylic extract of Tripanosoma cruzi as antigen, revealed a marked discrepancy in titers. The serum titer was expressed by the slope of the regresion line between amounts of serum and number of complement units needed for 50 per cent hemolysis

Since the inhibiter was soluble in water and almost insoluble in methanol, it could be isolated as an aqueous extract from trypanosomes previously treated by methanol. This hapten gave very weak complement fixation reactions with Chagasic sera. However, when added to the methylic antigen, a strong inhibition was observed. This inhibition was observed only with Chagasic sera, and not with sera from cases of syphilis, brucellosis, leprosy or schistosomiasis

An antigen free of inhibiter can be prepared by treating the dried trypanosomes, previously delipidized by benezen, with water saturated with chloroform. The extract trypanosomes are dried again and ...(AU)

In every case, the highest titers were obtained with the methylic antigen. It was observed also that the aqueous antigen, when added to the methylic antigen, produced a significant drop in titer. Everything suggested the presence of an hapten, able to combine specifically with Chagasic antibodies to form immune complexes which do not fix complement