RESUMO
Microbial (psychrotrophic, mesophilic aerobic bacteria and Enterobacteriacae counts), and chemical analysis [pH, total volatile bases nitrogen (TVB-N), lipid oxidation (Thiobarbituric acid reactive substance, TBARS)] of rainbow trout (Oncorynchus mykiss) fillets in air (control), vacuum and modified atmosphere packaging (MAP) with various gas mixtures conditions at 4+/-1 degrees C were determined. The gas mixtures evaluated were 100% CO2, 2.5% O2+7.5% N2+90% CO2 and 30% O2+30% N2+40% CO2. Psychrotrophic bacteria count was above 1 x 10(7) cfu/g on the 12th day in 100% CO2. However; mesophilic bacteria count was below 1 x 10(6) cfu/g at the end of the 14-day storage period. Enterobacteriaceae count was significantly lower in samples packaged with MAP. Lipid oxidation increased rapidly after 6 days of storage in the samples containing 30% O2. While minimum TBARS values were recorded in fillets containing 100% CO2 and vacuumed fillets, the lowest TVB-N values were obtained in fillets with 100% CO2.
Assuntos
Bactérias Aeróbias/isolamento & purificação , Enterobacteriaceae/isolamento & purificação , Embalagem de Alimentos/métodos , Oncorhynchus mykiss/microbiologia , Alimentos Marinhos/análise , Alimentos Marinhos/microbiologia , Animais , Bactérias Aeróbias/crescimento & desenvolvimento , Dióxido de Carbono/farmacologia , Contagem de Colônia Microbiana , Enterobacteriaceae/crescimento & desenvolvimento , Microbiologia de Alimentos , Peroxidação de Lipídeos , Controle de Qualidade , Substâncias Reativas com Ácido Tiobarbitúrico/análise , VácuoRESUMO
The effects of ammonia and urea on branchial carbonic anhydrase (CA) enzyme which plays a key role in ionoregulation, osmoregulation and acid-base balance of rainbow trout (Oncorhynchus mykiss) were investigated. CA activity of the control group for ammonia and urea was determined as 1285.7 ± 67.9 and 1261.7 ± 60.8EU/mg protein, respectively. The CA enzyme activities of the other groups were measured at 1, 2 and 3h after ammonia and urea applications. The corresponding activities of ammonia were 774.9 ± 68.8, 732.1 ± 48.6 and 768.1 ± 59.5EU/mg protein, respectively and that of urea were 769.3 ± 58.9, 638.2 ± 47.7 and 1108.1 ± 61.1EU/mg protein, respectively. The differences between the initial CA activities for the controls was not significantly (P > 0.01). The CA activities were significantly (P < 0.01) inhibited both in ammonia and urea group. However, the ammonia inhibited more than urea since there was significant differences between final values of gill CA activities.