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1.
Clin Exp Allergy ; 39(10): 1499-507, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19486035

RESUMO

BACKGROUND: To date, little information has been available about pulmonary artery pathology in asthma. The pulmonary artery supplies the distal parts of the lungs and likely represents a site of immunological reaction in allergic inflammation. The objective of this study was to describe the inflammatory cell phenotype of pulmonary artery adventitial inflammation in lung tissue from patients who died of asthma. METHODS: We quantified the different inflammatory cell types in the periarterial region of small pulmonary arteries in lung tissue from 22 patients who died of asthma [fatal asthma (FA)] and 10 control subjects. Using immunohistochemistry and image analysis, we quantified the cell density for T lymphocytes (CD3, CD4, CD8), B lymphocytes (CD20), eosinophils, mast cells (chymase and tryptase), and neutrophils in the adventitial layer of pulmonary arteries with a diameter smaller than 500 microm. RESULTS: Our data (median/interquartile range) demonstrated increased cell density of mast cells [FA=271.8 (148.7) cells/mm2; controls=177.0 (130.3) cells/mm2, P=0.026], eosinophils [FA=23.1 (58.6) cells/mm2; controls=0.0 (2.3) cells/mm2, P=0.012], and neutrophils [FA=50.4 (85.5) cells/mm2; controls=2.9 (30.5) cells/mm2, P=0.009] in the periarterial space in FA. No significant differences were found for B and T lymphocytes or CD4+ or CD8+ subsets. Chymase/tryptase positive (MCCT) mast cells predominated over tryptase (MCT) mast cells in the perivascular arterial space in both asthma patients and controls [MCCT/(MCCT+MCT)=0.91 (0-1) in FA and 0.75 (0-1) in controls, P=0.86]. CONCLUSIONS: Our results show that the adventitial layer of the pulmonary artery participates in the inflammatory process in FA, demonstrating increased infiltration of mast cells, eosinophils, and neutrophils, but not of T and B lymphocytes.


Assuntos
Arterite/metabolismo , Asma/metabolismo , Pulmão/metabolismo , Mastócitos/metabolismo , Artéria Pulmonar/metabolismo , Adolescente , Adulto , Idoso , Antígenos CD/metabolismo , Arterite/mortalidade , Arterite/patologia , Asma/mortalidade , Asma/patologia , Criança , Quimases/metabolismo , Feminino , Humanos , Inflamação/metabolismo , Inflamação/mortalidade , Inflamação/patologia , Leucócitos/metabolismo , Leucócitos/patologia , Pulmão/irrigação sanguínea , Pulmão/patologia , Masculino , Mastócitos/patologia , Pessoa de Meia-Idade , Artéria Pulmonar/patologia , Estudos Retrospectivos , Triptases/metabolismo
2.
Eur Respir J ; 32(1): 61-9, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18321931

RESUMO

There is an intimate relationship between the extracellular matrix (ECM) and smooth muscle cells within the airways. Few studies have comprehensively assessed the composition of different ECM components and its regulators within the airway smooth muscle (ASM) in asthma. With the aid of image analysis, the fractional areas of total collagen and elastic fibres were quantified within the ASM of 35 subjects with fatal asthma (FA) and compared with 10 nonfatal asthma (NFA) patients and 22 nonasthmatic control cases. Expression of collagen I and III, fibronectin, versican, matrix metalloproteinase (MMP)-1, -2, -9 and -12 and tissue inhibitor of metalloproteinase-1 and -2 was quantified within the ASM in 22 FA and 10 control cases. In the large airways of FA cases, the fractional area of elastic fibres within the ASM was increased compared with NFA and controls. Similarly, fibronectin, MMP-9 and MMP-12 were increased within the ASM in large airways of FA cases compared with controls. Elastic fibres were increased in small airways in FA only in comparison with NFA cases. There is altered extracellular matrix composition and a degradative environment within the airway smooth muscle in fatal asthma patients, which may have important consequences for the mechanical and synthetic functions of airway smooth muscle.


Assuntos
Asma/metabolismo , Matriz Extracelular/metabolismo , Metaloproteinases da Matriz/metabolismo , Músculo Liso/metabolismo , Inibidores Teciduais de Metaloproteinases/metabolismo , Adolescente , Adulto , Asma/patologia , Brônquios/metabolismo , Brônquios/patologia , Estudos de Casos e Controles , Matriz Extracelular/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Liso/patologia
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