Influência do tempo de equilíbrio sobre a qualidade do sêmen caprino após a descongelação / Influence of balance time on quality of buck semen after thawing

The animals were divided into three groups G1, G2, G3. Semen collected was diluted in Tris-yolk baseand packaged in 0.25 ml straws, a dose of 100 x 106 sperm. Semen samples were distributed in differentequilibrium time protocols. The group 1 (1h), group 2 (2 h), group 3 (3 h). Mean values for motility were19.37%, 16.87% and 16.25% respectively for G1, G2 and G3. To analyze (TTR) which consisted of placing asemen , and incubated at 37°C for a period of 120 minutes. The force sperm motility and sperm were evaluatedby TTR in different time: 0, 60, 120, 180 minutes. The indices obtained from motility and sperm vigor wereevaluated by PROC GLM (SAS, 2001) and proceeded to the 5% Tukey test. This study demonstrated effect theequilibration period, on the viability of goat semen after the freeze-thaw process, in which the semen exposed toless cooling time has a higher sperm viability.(AU)

Taxa de recuperação de oócitos de ovinos provenientes de abatedouros locais da região metropolitana de Teresina, Piauí, Brasil / Recovery rate of sheep oocyte from local abattoirs in metropolitan region of Teresina, Piauí, Brazil

In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.(AU)

Taxa de recuperação de oócitos caprinos oriundos de abatedouros localizados na microrregião homogênia de Teresina, Piauí, Brasil / Recovery rate goat oocytes from slaughterhouses located in the homogeneous micro-region of Teresina, Piauí, Brazil

The aim of this study was to evaluate the recovery rate of oocytes by OPU technique with needleattached syringe in goats from slaughterhouses located in the homogeneous micro-region of Teresina, Piauí. 68ovaries from 34 pubertal goats without defined breed (SRD) from local abattoirs were used. After slaughter, theovaries were immediately transported to the laboratory in thermos physiological saline (0.9% NaCl) heated at35 °C plus 30 ug/ml gentamicin sulfate. Soon after it was held aspiration of oocytes and the identification andclassification of structures was subsequently conducted. It was recovered a total of 42 COCs, the average ofoocytes recovered by females 1.23. The low recovery rate obtained in this study may be due to low bodycondition score in which the animals were at the time of the examination.(AU)

Qualidade do sêmen caprino diluído em tris contendo diferentes formulações do extrato bruto de mauritia flexuoxa / Sperm quality goat diluted in three different formulations containing extract of crude mauritia flexuoxa

The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.(AU)

Criopreservação do sêmen caprino: efeito do tempo de equilíbrio sobre a qualidade do sêmen após a descongelação / Cryopreservation of goat semen: Balancing time effect on semen quality after thawing

Which was used sixteen samples of adult goats semen collected using an artificial vagina weresubjected to cryopreservation in order to verify the influence of equilibration time at a temperature of 5 °C onthe freezability of goat semen. Were distributed in the different protocols equilibrium time: 1h equilibration time(G1); 2 h equilibration time (G2); 3h equilibration time (G3).After this process the samples were placed inliquid nitrogen vapor , and maintained for 20 min . The end was made the freezing process in cryogenics tank .Thawing was performed in a water bath at 37°C for 30 seconds. The averages for motility were 19.375 %,16.875 % and 16.250 % respectively for G1, G2 and G3. This study demonstrated effect of equilibration period ,on the viability of goat semen after the process of freeze-thaw, where the group of smaller G1 exposure time wasless deleterious effects.(AU)

Criopreservação do sêmen caprino: efeito do tempo de equilíbrio sobre a qualidade do sêmen após a descongelação / Cryopreservation of goat semen: Balancing time effect on semen quality after thawing

Which was used sixteen samples of adult goats semen collected using an artificial vagina weresubjected to cryopreservation in order to verify the influence of equilibration time at a temperature of 5 °C onthe freezability of goat semen. Were distributed in the different protocols equilibrium time: 1h equilibration time(G1); 2 h equilibration time (G2); 3h equilibration time (G3).After this process the samples were placed inliquid nitrogen vapor , and maintained for 20 min . The end was made the freezing process in cryogenics tank .Thawing was performed in a water bath at 37°C for 30 seconds. The averages for motility were 19.375 %,16.875 % and 16.250 % respectively for G1, G2 and G3. This study demonstrated effect of equilibration period ,on the viability of goat semen after the process of freeze-thaw, where the group of smaller G1 exposure time wasless deleterious effects.

Influência do tempo de equilíbrio sobre a qualidade do sêmen caprino após a descongelação / Influence of balance time on quality of buck semen after thawing

The animals were divided into three groups G1, G2, G3. Semen collected was diluted in Tris-yolk baseand packaged in 0.25 ml straws, a dose of 100 x 106 sperm. Semen samples were distributed in differentequilibrium time protocols. The group 1 (1h), group 2 (2 h), group 3 (3 h). Mean values for motility were19.37%, 16.87% and 16.25% respectively for G1, G2 and G3. To analyze (TTR) which consisted of placing asemen , and incubated at 37°C for a period of 120 minutes. The force sperm motility and sperm were evaluatedby TTR in different time: 0, 60, 120, 180 minutes. The indices obtained from motility and sperm vigor wereevaluated by PROC GLM (SAS, 2001) and proceeded to the 5% Tukey test. This study demonstrated effect theequilibration period, on the viability of goat semen after the freeze-thaw process, in which the semen exposed toless cooling time has a higher sperm viability.

Qualidade do sêmen caprino diluído em tris contendo diferentes formulações do extrato bruto de mauritia flexuoxa / Sperm quality goat diluted in three different formulations containing extract of crude mauritia flexuoxa

The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.

Taxa de recuperação de oócitos caprinos oriundos de abatedouros localizados na microrregião homogênia de Teresina, Piauí, Brasil / Recovery rate goat oocytes from slaughterhouses located in the homogeneous micro-region of Teresina, Piauí, Brazil

The aim of this study was to evaluate the recovery rate of oocytes by OPU technique with needleattached syringe in goats from slaughterhouses located in the homogeneous micro-region of Teresina, Piauí. 68ovaries from 34 pubertal goats without defined breed (SRD) from local abattoirs were used. After slaughter, theovaries were immediately transported to the laboratory in thermos physiological saline (0.9% NaCl) heated at35 °C plus 30 ug/ml gentamicin sulfate. Soon after it was held aspiration of oocytes and the identification andclassification of structures was subsequently conducted. It was recovered a total of 42 COCs, the average ofoocytes recovered by females 1.23. The low recovery rate obtained in this study may be due to low bodycondition score in which the animals were at the time of the examination.

Taxa de recuperação de oócitos de ovinos provenientes de abatedouros locais da região metropolitana de Teresina, Piauí, Brasil / Recovery rate of sheep oocyte from local abattoirs in metropolitan region of Teresina, Piauí, Brazil

In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.