RESUMO
The factors potentiating the efficacy of fusion of protoplasts of Bacillus subtilis were studied. A 50-fold increase in the yield of primary prototrophs was provided by 90-minute preincubation of protoplasts in a liquid hyperbaric medium and 10-minute treatment with trypsin. Increased efficacy of fusion of protoplasts depended on their regeneration percentage.
Assuntos
Bacillus subtilis/genética , Protoplastos/fisiologia , Aminoácidos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Hibridização Genética , Soluções Hipertônicas , Protoplastos/efeitos dos fármacos , Tripsina/farmacologiaRESUMO
The experience with the surgical treatment of 97 patients subjected to late repeated operations on the lungs and pleura is generalized in this work. 68% of these patients had pleural empyema and bronchial fistulae. The authors prefer the radical surgery to pulmonectomy, pleuropulmonectomy, decortication, lung resection and repeated amputation of the main bronchus. Fatality in the late repeated operations was noted in 3.09% of cases and complications--in 10.3%.