RESUMO
The authors presented the aspects of diagnostics of alcohol dependence paying special attention to its laboratory and biochemical side. Biological sensitive and specific markers of alcohol dependence and abuse: N-acetyl-beta-hexosoaminidase, carbohydrate deficient transferrin and protein-acetaldehyde adducts were also presented.
Assuntos
Alcoolismo/diagnóstico , Detecção do Abuso de Substâncias/métodos , Acetaldeído/sangue , Alcoolismo/sangue , Biomarcadores/sangue , Humanos , Testes de Função Hepática , Sensibilidade e Especificidade , Transferrina/análise , beta-N-Acetil-Hexosaminidases/sangueRESUMO
This article discusses biochemical changes of ethyl alcohol in human organism, concentrating especially on the negative influence on the metabolism of liver. The authors describe the process of oxidation of alcohol with alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) and emphasize the role of ADH i ALDH isoenzymes in creating individual tolerance of ethanol. Two other ways of ethanol metabolism are also presented. These are: microsomal ethanol oxidation system (MEOS) connected with cytochrome P-450 and peroxisome catalase system. The article also describes the influence of alcohol and its products of metabolism on the structure of liver proteins, on different metabolic processes taking place in this organ, and on the changes in the immunological system in the course of the alcoholic liver disease. Moreover, the authors present some information about the changes in histopathological picture of liver as the result of alcohol abuse.
Assuntos
Alcoolismo/metabolismo , Etanol/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Humanos , Fígado/patologiaRESUMO
The activity of N-acetyl-beta-hexosaminidase was found to be significantly higher in the placentas collected after delivery from women in puerperium with symptoms of prolonged pregnancy or complicated by EPH gestosis, than in placentas from normal pregnancy. Isoelectrofocusing of placenta homogenates showed the presence of isoenzymes A, P and B of N-acetyl-beta-hexosaminidase. Different isoenzyme A patterns in homogenates were observed in placentas obtained from normal and prolonged pregnancies and in those complicated by EPH gestosis.
Assuntos
Isoenzimas/metabolismo , Complicações na Gravidez/enzimologia , beta-N-Acetil-Hexosaminidases/metabolismo , Estudos de Casos e Controles , Feminino , Humanos , Focalização Isoelétrica , Placenta/enzimologia , Pré-Eclâmpsia/enzimologia , Gravidez , Gravidez Prolongada/metabolismoRESUMO
Some cyclophosphamide toxic effects on lung tissue are presented. Cyclophosphamide metabolism, pathogenesis of lung damage and morphological lung tissue changes caused by that agent were characterized. Attention was focused on BAL evaluation as a useful method in the monitoring of lung tissue damage degree.
Assuntos
Antineoplásicos Alquilantes/toxicidade , Ciclofosfamida/toxicidade , Pulmão/efeitos dos fármacos , Fibrose Pulmonar/induzido quimicamente , Animais , Antineoplásicos Alquilantes/efeitos adversos , Antineoplásicos Alquilantes/metabolismo , Biotransformação , Ciclofosfamida/efeitos adversos , Ciclofosfamida/metabolismo , Humanos , Pulmão/ultraestrutura , Fibrose Pulmonar/patologia , RatosRESUMO
Inhibition by ethanol of the activities of lysosomal exoglycosidases in stomach, small intestine, liver and brain of rats exposed to cadmium (Cd2+) was determined. Out of the glycosidases tested the most distinct effect of Cd2+ and ethanol administered to the rats in vivo was observed in the small intestinal mucosa in a decreasing order: N-acetyl-beta-hexosaminidase, beta-galactosidase and alpha-fucosidase.
Assuntos
Cádmio/toxicidade , Etanol/toxicidade , Glicosídeo Hidrolases/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/enzimologia , Sinergismo Farmacológico , Glicoconjugados/metabolismo , Glicosídeo Hidrolases/antagonistas & inibidores , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/enzimologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Lisossomos/enzimologia , Masculino , Manosidases/antagonistas & inibidores , Manosidases/metabolismo , Ratos , Ratos Wistar , Estômago/efeitos dos fármacos , Estômago/enzimologia , alfa-L-Fucosidase/antagonistas & inibidores , alfa-L-Fucosidase/metabolismo , alfa-Manosidase , beta-Galactosidase/antagonistas & inibidores , beta-Galactosidase/metabolismo , beta-N-Acetil-Hexosaminidases/antagonistas & inibidores , beta-N-Acetil-Hexosaminidases/metabolismoRESUMO
Exoglycosidases catabolize glycoconjugates (proteoglycans, glycolipids and glycoproteins) at a rate depending on tissue and pathological changes. We determined exoglycosidase activity in successive sections of alimentary tract, spleen, liver and kidney of rats subjected to hemorrhagic shock. We found significant difference in exoglycosidase activity between alimentary tract segments in control animals and no significant changes in homologous tissues of the shocked rats comparing with control.
