1.
J Bacteriol
; 192(6): 1730-4, 2010 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-20081027
RESUMO
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.