RESUMO
An outbreak of infection with a high rate of mortality has been detected in an industrial rabbitry in Spain. Aeromonas hydrophila was isolated in pure culture from liver, lung, heart and spleen of ill and moribund animals. All the isolates belonged to O:11 serogroup, autogglutinated after boiling, were resistant to the bactericidal action of the fresh rabbit serum and did not agglutinate in acriflavine. They were producers of hemolysins and proteases but were not enterotoxigenic.
Assuntos
Aeromonas hydrophila , Surtos de Doenças/veterinária , Infecções por Bactérias Gram-Negativas/veterinária , Coelhos/microbiologia , Aeromonas hydrophila/classificação , Aeromonas hydrophila/isolamento & purificação , Animais , Animais Domésticos , Bacteriemia/epidemiologia , Bacteriemia/terapia , Bacteriemia/veterinária , Vacinas Bacterianas , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/terapia , Coração/microbiologia , Fígado/microbiologia , Pulmão/microbiologia , Sorotipagem , Espanha , Baço/microbiologiaRESUMO
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to assemble to form virus like particles, but was antigenically similar to the corresponding viral protein obtained from purified virions. A VP60-dose study showed that the recombinant protein was able to elicit a protective response in rabbits against a nasal challenge with 100 LD50 of RHDV. The effective dose able to protect 50% of the animals in the absence of adjuvant was found to be 10-25 micrograms of recombinant VP60.
Assuntos
Infecções por Caliciviridae/veterinária , Vírus da Doença Hemorrágica de Coelhos/imunologia , Proteínas Estruturais Virais/imunologia , Vacinas Virais/imunologia , Animais , Infecções por Caliciviridae/imunologia , Infecções por Caliciviridae/prevenção & controle , Linhagem Celular , Regulação Viral da Expressão Gênica , Vírus da Doença Hemorrágica de Coelhos/genética , Vírus da Doença Hemorrágica de Coelhos/fisiologia , Nucleopoliedrovírus/genética , Coelhos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Spodoptera/citologia , Vacinação , Proteínas Estruturais Virais/genética , Vacinas Virais/genética , Vírion/fisiologia , Montagem de VírusRESUMO
Results are presented for the large-scale use of a tissue vaccine, inactivated with beta-propiolactone and containing aluminium hydroxide adjuvant, against viral haemorrhagic disease of rabbits. The kinetics of haemagglutination-inhibiting antibodies over eighteen months, the response of vaccinated animals to challenge infection with field virus between six and fifteen months after vaccination, the serological response to revaccination, and the immunological mechanisms involved in primary vaccination and revaccination were investigated.
