RESUMO
The proteoglycans in the submandibular salivary glands of castrated male Wistar rats were studied before and after the daily administration of testosterone propionate (TP) for one month. Castration decreased the weight of the glands and their uronic acid content. The administration of TP reversed these effects. Chromatographic separation of the uronic acid fractions was performed on cellulose microcolumns. The principal fractions were hyaluronic acid, heparan sulfate and dermatan sulfate. There were also changes in the physical properties of the proteoglycans. Castration decreased the range of distribution of molecular weight and the density, while the lateral chains of smaller length disappeared. TP administration to castrated rats reversed these effects.
Assuntos
Proteoglicanas/análise , Proteínas e Peptídeos Salivares/análise , Glândula Submandibular/efeitos dos fármacos , Testosterona/farmacologia , Animais , Castração , Fracionamento Químico , Sulfatos de Condroitina/análise , Cromatografia em Agarose , Glicosaminoglicanos/análise , Masculino , Ratos , Ratos Endogâmicos , Glândula Submandibular/química , Testosterona/administração & dosagem , Ácidos Urônicos/análiseRESUMO
In studies performed on male Wistar rats, castration induced atrophy of the prostate with a marked increase in the uronic acid content. The administration of testosterone propionate to castrated rats produced opposite effects. Fractionation of the glycosaminoglycans on cellulose microcolumns showed that the changes in uronic acid content in the dorsolateral lobes were due to variations in hyaluronic acid, chondroitin-4-sulfate, and dermatan sulfate, but in the ventral lobes, there were changes in all the chromatographic fractions. There were also changes in the physical properties of proteoglycans. In the ventral lobes, castration induced a wider distribution of molecular weight, increased density, and predominance of lateral chains of greater size. In the dorsolateral lobes, there was a decrease in molecular weight and density of proteoglycans and in the length of lateral chains. Opposite results were obtained when testosterone propionate was given to castrated rats. It is postulated that the effects of androgens upon prostatic growth would depend on an interrelationship between epithelium and stroma mediated by the proteoglycans.
Assuntos
Orquiectomia , Próstata/metabolismo , Proteoglicanas/metabolismo , Testosterona/farmacologia , Animais , Centrifugação com Gradiente de Concentração , Cromatografia , Glicosaminoglicanos/metabolismo , Masculino , Peso Molecular , Concentração Osmolar , Ratos , Ratos Endogâmicos , Ácidos Urônicos/metabolismoRESUMO
DL-isoproterenol hydrochloride (1 mg/kg body weight/day) was subcutaneously administered to male A2G mice during 15 or 45 days. The sympathetic superior cervical ganglion of each mouse was resected on the right side, two days before beginning the injections. At the end of the injection period, the I131 submaxillary/plasma ratios and I131 thyroid uptake (%) were measured 3 hours after a tracer dose. The administration of isoproterenol induced marked hypertrophy in both normal and denervated submaxillary glands. At 15 days the I131 submaxillary/plasma ratios of the isoproterenol treated mice were slightly decreased on the normal side and were not modified on the denervated side. At 45 days the I131 submaxillary/plasma ratios were markedly decreased on both sides. The thyroid weight and I131 uptake were not modified by the isoproterenol treatment.
Assuntos
Isoproterenol/farmacologia , Glândula Submandibular/efeitos dos fármacos , Animais , Hipertrofia , Radioisótopos do Iodo , Masculino , Camundongos , Glândula Submandibular/metabolismo , Simpatectomia , Glândula Tireoide/fisiologiaRESUMO
DL-isoproterenol hydrochloride (1 mg/kg body weight/day) was subcutaneously administered to male A2G mice during 15 or 45 days. The sympathetic superior cervical ganglion of each mouse was resected on the right side, two days before beginning the injections. At the end of the injection period, the I131 submaxillary/plasma ratios and I131 thyroid uptake (
) were measured 3 hours after a tracer dose. The administration of isoproterenol induced marked hypertrophy in both normal and denervated submaxillary glands. At 15 days the I131 submaxillary/plasma ratios of the isoproterenol treated mice were slightly decreased on the normal side and were not modified on the denervated side. At 45 days the I131 submaxillary/plasma ratios were markedly decreased on both sides. The thyroid weight and I131 uptake were not modified by the isoproterenol treatment.
RESUMO
DL-isoproterenol hydrochloride (1 mg/kg body weight/day) was subcutaneously administered to male A2G mice during 15 or 45 days. The sympathetic superior cervical ganglion of each mouse was resected on the right side, two days before beginning the injections. At the end of the injection period, the I131 submaxillary/plasma ratios and I131 thyroid uptake (
) were measured 3 hours after a tracer dose. The administration of isoproterenol induced marked hypertrophy in both normal and denervated submaxillary glands. At 15 days the I131 submaxillary/plasma ratios of the isoproterenol treated mice were slightly decreased on the normal side and were not modified on the denervated side. At 45 days the I131 submaxillary/plasma ratios were markedly decreased on both sides. The thyroid weight and I131 uptake were not modified by the isoproterenol treatment.
RESUMO
The effects of cyproterone and of tamoxifen citrate upon the hair waves were studied in normal and castrated C57 mice. Cyproterone (1 mg/day) had no effect upon the hair waves in normal male mice and produced a slight inhibition of the diffuse growth induced by their castration. Tamoxifen citrate (1 mg/day) had no effect upon the hair waves in normal female mice and produced a marked inhibition (0.2 to 1 mg/day) upon the diffuse hair growth induced by their castration. The differences between the effects of castration and the effect of these drugs can be explained in the case of cyproterone by a slight agonistic androgenic effect and in the case of tamoxifen citrate by a marked agonistic estrogenic effect upon the hair follicles in mice.
Assuntos
Ciproterona/farmacologia , Cabelo/efeitos dos fármacos , Tamoxifeno/farmacologia , Animais , Castração , Feminino , Cabelo/crescimento & desenvolvimento , Masculino , Camundongos , Camundongos Endogâmicos C57BLRESUMO
The effects of cyproterone and of tamoxifen citrate upon the hair waves were studied in normal and castrated C57 mice. Cyproterone (1 mg/day) had no effect upon the hair waves in normal male mice and produced a slight inhibition of the diffuse growth induced by their castration. Tamoxifen citrate (1 mg/day) had no effect upon the hair waves in normal female mice and produced a marked inhibition (0.2 to 1 mg/day) upon the diffuse hair growth induced by their castration. The differences between the effects of castration and the effect of these drugs can be explained in the case of cyproterone by a slight agonistic androgenic effect and in the case of tamoxifen citrate by a marked agonistic estrogenic effect upon the hair follicles in mice.
RESUMO
Large masses that looked like tumours were found in place of the ovaries in rats with isotransplants of pituitaries, 11 months after this procedure. They were interpreted as being due to masive bilateral cystic hyperplasia and hypertrophy of both ovaries.
Assuntos
Ovário/patologia , Hipófise/transplante , Animais , Feminino , Hipertrofia/patologia , Hipófise/fisiologia , Prolactina/sangue , Ratos , Transplante IsogênicoRESUMO
The administration of indomethacin to male A2G mice induced a marked decrease in the 131I uptake by the thyroid, but it did not influence the 131I uptake by the submaxillary glands.
Assuntos
Indometacina/farmacologia , Iodo/metabolismo , Glândula Submandibular/efeitos dos fármacos , Glândula Tireoide/efeitos dos fármacos , Animais , Indometacina/administração & dosagem , Radioisótopos do Iodo , Masculino , Camundongos , Glândula Submandibular/metabolismo , Glândula Tireoide/metabolismoRESUMO
The effect of prostaglandins upon the diffuse hair wave induced by gonadectomy was studied in male C3H mice. The drugs used were Prostaglandin E1 and Prostaglandin F2alpha, tromethamine salt, which were administered twice a day intraperitoneally during 22 days, in daily doses from 1 to 6 micrograms. The animals had their back clipped and were castrated at the beginning of each experiment. At the end of the 22 day experimental period while all the castrated control mice were completely covered by hair, in the castrated prostaglandin treated mice a marked inhibition of the hair growth was noticed.
Assuntos
Cabelo/efeitos dos fármacos , Prostaglandinas/farmacologia , Animais , Castração , Cabelo/crescimento & desenvolvimento , Injeções Intraperitoneais , Masculino , Camundongos , Camundongos Endogâmicos C3H , Prostaglandinas E/farmacologia , Prostaglandinas F/farmacologia , Pele/efeitos dos fármacos , Estimulação Química , Testículo/cirurgia , Fatores de TempoRESUMO
The effect of prostaglandins upon the diffuse hair wave induced by gonadectomy was studied in male C3H mice. The drugs used were Prostaglandin E1 and Prostaglandin F2alpha, tromethamine salt, which were administered twice a day intraperitoneally during 22 days, in daily doses from 1 to 6 micrograms. The animals had their back clipped and were castrated at the beginning of each experiment. At the end of the 22 day experimental period while all the castrated control mice were completely covered by hair, in the castrated prostaglandin treated mice a marked inhibition of the hair growth was noticed.
RESUMO
The effect of prostaglandins upon the diffuse hair wave induced by gonadectomy was studied in male C3H mice. The drugs used were Prostaglandin E1 and Prostaglandin F2alpha, tromethamine salt, which were administered twice a day intraperitoneally during 22 days, in daily doses from 1 to 6 micrograms. The animals had their back clipped and were castrated at the beginning of each experiment. At the end of the 22 day experimental period while all the castrated control mice were completely covered by hair, in the castrated prostaglandin treated mice a marked inhibition of the hair growth was noticed.