1.
Anal Chem
; 85(3): 1637-41, 2013 Feb 05.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23259401
RESUMO
We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (â¼95%) with low toxicity (cell viability â¼85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations.