Estimation of dust concentration by a novel machine vision system.

The dust phenomenon is one of the main environmental problems that it reversely affects human health and economical and social activities. In the present research, a novel algorithm has been developed based on image processing to estimate dust concentration. An experimental setup was implemented to create airborne dust with different concentration values from 0 to 2750 µg.m-3. The images of the different dust concentration values were acquired and analyzed by image processing technique. Different color and texture features were extracted from various color spaces. The extracted features were used to develop single and multivariable models by regression method. Totally 285 single variable models were obtained and compared to select efficient features among them. The best single variable model had a predictive accuracy of 91%. The features were used for multivariable modeling and the best model was selected with a predictive accuracy of 100% and a mean squared error of 1.44 × 10-23. The results showed the high ability of the developed machine vision system for estimating dust concentration with high speed and accuracy.

A Resuscitation Option for Upper Airway Occlusion Based on Bolus Transtracheal Lung Inflation.

BACKGROUND: Acute laryngospasm sufficient to cause obstructive apnea is a medical emergency that can be difficult to manage within the very short time available for establishing an airway. We have presented substantial evidence that laryngospasm-based obstructive apnea is the cause of sudden death in epilepsy, and airway management is particularly challenging during seizure activity. OBJECTIVE: We sought to determine if the transtracheal delivery of a bolus of oxygen or room air below the level of an obstruction to inflate the lungs could be an effective method to prolong the time available for responders seeking to establish a stable airway, and, if so, what could be learned about optimization of delivery parameters from a rat model. METHODS: Rats were fitted with a t-shaped tracheal tube for controlling access to air and for measuring airway pressures. After respiratory arrest from simulated laryngospasm, bolus transtracheal lung inflation with a volume of gas equivalent to half the vital capacity was delivered to the closed respiratory system as the only resuscitation step. RESULTS: Bolus lung inflation was sufficient for resuscitation, improving cardiac function and re-establishing adequate oxygen status to support life. Inflation steps could be repeated and survival times were approximately 3 times that of non-inflated lungs. CONCLUSION: The properties and consequences of bolus lung inflation are described as a foundation for procedures or devices that can be useful in cases of severe laryngospasm and other cases of upper airway obstruction. LEVEL OF EVIDENCE: 3.

Systematic review of surgery for persistent pediatric tracheocutaneous fistula.

OBJECTIVES: To compare the treatment outcomes and adverse event rates for primary closure compared to simple excision with healing by secondary intention for persistent tracheocutaneous fistula (TCF) in children. STUDY DESIGN: Systematic review. METHODS: Systematic review using Preferred Reporting Items for Systematic Reviews and Meta-Analyses reporting standards. MEDLINE, Embase, Cochrane Library, and manual search were used to identify articles. Inclusion criteria were case series or comparative studies of surgery for persistent TCF in children under age 18 years. Exclusion criteria were age 18 years or older, duplicate patient series, case series with less than two patients, or case reports. Data were pooled using random effects meta-analysis to assess outcomes, adverse events, and comparative effectiveness. RESULTS: We identified 14 articles with 413 patients treated with excision with primary closure and 233 patients treated with excision with closure by secondary intention. Pooled success rates were 95.7% with primary closure (95% confidence interval [CI]: 93.1% to 97.4%) and 92.7% with secondary intention (95% CI: 88.4% to 95.4%). Subcutaneous emphysema or urgent airway problems were uncommon, occurring in 3.8% and 3.6% of patients, respectively. Five studies had data suitable for comparative meta-analysis, which showed no differences by closure technique for treatment success (P = .480), overall complications (P = .551), need for revision surgery (P = .624), or the incidence of subcutaneous emphysema or pneumothorax (P = .512), urgent airway problems (P = .126), wound infection (P = 1.00), or wound dehiscence or fistula (P = .818). CONCLUSIONS: There are no differences in the rates of success or complications with surgical closure of TCF in children, whether performed by primary closure or by secondary intention. Both techniques have high success rates with a low incidence of serious complications. Laryngoscope, 127:241-246, 2017.

Relationship of eosinophils and plasma cells to biofilm in chronic rhinosinusitis.

BACKGROUND: This study investigates the relationship of eosinophils and plasma cells to biofilm in chronic rhinosinusitis (CRS). A prospective observational study was performed at the Keck Hospital, University of Southern California, Department of Otolaryngology, Los Angeles, CA. METHODS: A total of 29 patients, 20 undergoing endoscopic sinus surgery for CRS and 9 control patients undergoing septoplasty for nasal obstruction without history or evidence of CRS, were included in this study. Contiguous sinonasal mucosa sample sections were examined by hematoxylin and eosin (H&E), fluorescence in situ hybridization (FISH), and immunohistochemistry (IHC) for biofilm, microbes, eosinophil major basic protein (EMBP), and cluster designation 27 (CD27). EMBP and CD27 were used as eosinophil and plasma cell markers, respectively. RESULTS: Biofilm was visualized in 15 of 20 patients with CRS on H&E sections, confirmed by microbial presence using FISH. Biofilm was not identified in tissue samples of the nine control patients. On IHC analysis, CD27 and EMBP expression were significantly higher in patients with CRS compared with control (p < 0.05) and had greater expression in biofilm-positive patients compared with biofilm-negative patients. Nasal polyps correlated with higher expression of CD27 and EMBP, but in CRS patients without polyps CD27 and EMBP was also significantly greater in biofilm-positive specimens compared with biofilm-negative specimens. CONCLUSION: Biofilm presence in CRS appears to correlate to host inflammatory response involving plasma cell and eosinophil recruitment.