Effect of mercury in the influx and efflux of nutrients in the microalga Desmodesmus armatus.

Anthropogenic activities such as mining and the metallurgical industry are the main sources of mercury contamination. Mercury is one of the most serious environmental problems in the world. This study aimed to investigate, using experimental kinetic data, the effect of different inorganic mercury (Hg2+) concentrations on the response of microalga Desmodesmus armatus stress. Cell growth, nutrients uptake and mercury ions from the extracellular medium, and oxygen production were determined. A Compartment Structured Model allowed elucidating the phenomena of transmembrane transport, including influx and efflux of nutrients, metal ions and bioadsorption of metal ions on the cell wall, which are difficult to determine experimentally. This model was able to explain two tolerance mechanisms against mercury, the first one was the adsorption of Hg2+ions onto the cell wall and the second was the efflux of mercury ions. The model predicted a competition between internalization and adsorption with a maximum tolerable concentration of 5.29 mg/L of HgCl2. The kinetic data and the model showed that mercury causes physiological changes in the cell, which allow the microalga to adapt to these new conditions to counteract the toxic effects. For this reason, D. armatus can be considered as a Hg-tolerant microalga. This tolerance capacity is associated with the activation of the efflux as a detoxification mechanism that facilitates the maintenance of the osmotic balance for all the modeled chemical species. Furthermore, the accumulation of mercury in the cell membrane suggests the presence of thiol groups associated with its internalization, leading to the conclusion that metabolically active tolerance mechanisms are dominant over passive ones.

Reducing cadmium bioaccumulation in Theobroma cacao using biochar: basis for scaling-up to field.

The intake of Cd-enriched food is the main Cd pathway for the nonsmoking population. In some cases, Cd bioaccumulates in edible plant parts which comprise risk to consumers, because of Cd is a harmful heavy metal that can cause potent environmental and health hazards. For instance, Cd enrichment of cacao seeds have led to Cd enrichment of cacao-based products. In Latin America and the Caribbean, Cd bioaccumulation in cacao seeds occurs in different regions with diverse edaphoclimatic conditions, which makes it difficult to select soil remediation alternatives. Limited resources require that potential amendments must be carefully investigated through laboratory and/or greenhouse conditions before scaling up to field experiments. In this study, we evaluated the effectiveness of four biochars: coffee-, quinoa-, and inoculated- and palm-biochar, derived from three feedstocks: coffee husk, quinoa straw, and oil palm residues, respectively. Biochars were applied in two rates (1 and 2% w/w) in two soils, one moderately acidic and one slightly alkaline (Cd-spiked and non-spiked). CCN-51 cacao plants were used for the greenhouse experiment. After 130 days, biometric parameters, the bioavailability of Cd in the soil, and the concentration of Cd and mineral nutrients in the plants were measured. Quinoa biochar at the 2% significantly decreased (P < 0.01), by ∼71%, bioavailable Cd in moderately acidic and slightly alkaline soils, and leaf-Cd by ∼48%. Soil pH, electrical conductivity, and effective cation exchange capacity were significantly (P < 0.01) correlated with bioavailable soil and leaf-Cd. Biochar characteristics, such as ash contents, basic cations content, and surface functional groups could be used as indicators for the selection of biochars to reduce Cd uptake by cacao. Additionally, application of quinoa derived biochar provided P and K, which could increase productivity to offset mitigation costs. Overall, incorporation of quinoa biochar at 2% rate is effective for lowering bioavailable Cd in different soil types which reduces leaf-Cd in cacao plants.

A proteomic approach to the mechanisms underlying activation of aluminium resistance in roots of Urochloa decumbens.

The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72-96h and is preceded by a sensitive phase (24-48h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96h exposure to 0 or 200µM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase. Only 11 proteins revealed Al-induced abundance differences; six were identified. After 24h, phenylalanine ammonium lyase (PAL), methionine synthase (MS), and deoxymugineic acid synthase (DMAS) decreased, while acid phosphatase (APase) abundance increased. Coincident with growth recovering, PAL and MS, but not DMAS, returned to initial levels. After 96h, γ­carbonic anhydrase (γ­CA) and adenylate kinase (AK) along with two unidentified proteins were more abundant. In conclusion, few protein changes characterize the initial response to Al in signalgrass. During the alarm phase, changes are related to P-mobilization, downregulation of Fe-acquisition, reduction of phenolic biosynthesis, and small stimulation of organic acid exudation. After recovering (resistant phase), biosynthesis of phenolics and methionine, but not Fe-mobilization are re-established. Full expression of Al-resistance is characterized by enhanced γ­CA mediating mitochondrial complex I assembly and increased AK abundance indicating higher root respiration and better provision of ADP and Mg2+ to ATP synthase, respectively. The unidentified proteins and the specific role of γ­CA in Al resistance of U. decumbens will centre future research.

Aluminium-induced changes in root epidermal cell patterning, a distinctive feature of hyperresistance to Al in Brachiaria decumbens.

Brachiaria, a genus of forage grasses of African origin, is gaining considerable importance because of both its nutritional value and its high stress resistance. An extraordinary resistance to Al toxicity has been reported in B. decumbens. The mechanisms of this hyperresistance are still unknown. This study explores the localization of Al in two contrasting Brachiaria species, the hyperresistant B. decumbens and the less resistant B. brizantha. Scanning Electron Microscope/Energy Dispersive Spectrometry, confocal fluorescence microscopy and optical microscopy of lumogallion or morin-stained roots was performed. Species differences in Al resistance were evident at 32 µM Al(3+) activity in low ionic strength full nutrient solution containing Si. Roots of B. decumbens accumulated less Al than those of B. brizantha. Moreover, location and Al form seemed different. In B. decumbens Al accumulation was localized in hot spots of high Al concentrations. These sites with high Al accumulation mainly correspond to root hairs. B. brizantha exhibited a more even distribution of Al in cell walls of the root tip. Analysis of soluble phenolic substances in roots revealed species differences in response to Al. An Al-induced increase of chlorogenic acid concentrations was found in B. decumbens but not in B. brizantha. Taken together the results suggest a possible role for chlorogenic acid as a primer for changes in root epidermal cell patterning that may contribute to the Al hyperresistance in B. decumbens.

Los estudiantes de enfermería de la Universidad de Antioquiaen la real -red de enfermería de América Latina- / Universidad de Antioquia nursing students in REAL - Latin America's nursing net-

Este articulo presenta la propuesta que los estudiantes de VII nivel de la Facultad de Enfermería de la Universidad de Antioquia elaboramos, para tener una participación interactiva en la Red de Enfermería de América Latina, REAL, con el fin de potenciar el desarrollo de la profesión de Enfermería, de acuerdo con la tecnología disponible, la necesidad constante de actualización y los beneficios del trabajo en red.El trabajo en red aporta grandes ventajas a los estudiantes y a la profesión de Enfermería; con él, el ejercicio profesional se puede fortalecer, así como el crecimiento personal. Para que haya red tiene que haber personas dispuestas a dialogar con otras que no conocemos, que físicamente no están y que podemos eludir simplemente apagando el computador. En el primer Congreso Latinoamericano de Estudiantes de Bibliotecología y Ciencias de la Información realizado en Medellín en 1998 , se identificaron cuatro niveles para el trabajo en red: intercambio de Información, coordinación, cooperación y colaboración.Partiendo de esto, las estrategias que se plantean para la interacción de estudiantes, docentes y profesionales en Enfermería de diversas culturas dentro de la REAL , tiene en cuenta tres aspectos fundamentales a la hora de la planeación y estructuración de la propuesta, estos son: Iniciar, Sostener y Evaluar ; se presentaran momentos en los que una determinada estrategia sirva para dos o mas fines . Las estrategias planteadas son : 1- Diseño de un póster REAL. 2- Crear un portal llave para la REAL . 3- Abrir un espacio de interacción estudiantil en la REAL. 4- Publicación : Estudiantes en red. 5- Celebrar un encuentro REAL. 6- reunión Académica de Enfermería RAE.