Increased interaction of proteins in nuclear extract from mouse liver and lung tumors with TATA-containing oligonucleotide.

Increased TATA-binding activity of proteins in nuclear extracts from murine hepatocarcinoma HA-1 and murine Lewis lung adenocarcinoma was demonstrated. The dependence of the amount of formed complexes on protein concentration, displacement of labeled 32P-TATA-containing oligonucleotide by its unlabeled analog, and weak interaction with an oligonucleotide containing damaged TATA box confirm specificity of the formed complexes.