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2.
Nanotechnology ; 26(45): 455703, 2015 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-26487422

RESUMO

Downsizing to the nanoscale has opened up a spectrum of new magnetic phenomena yet to be discovered. In this context, we investigate the magnetic properties of Ni clusters embedded in a metallic Ag matrix. Unlike in Ni free-standing clusters, where the magnetic moment increases towards the atomic value when decreasing the cluster size, we show, by tuning the Ni cluster size down to the sub-nanoscale, that there is a size limit below which the clusters become non-magnetic when embedded in Ag. To this end, we have fabricated by DC-sputtering a system composed of sub-nanometer sized and non interacting Ni clusters embedded into a Ag matrix. A thorough experimental characterization by means of structural techniques (x-ray diffraction, x-ray absorption spectroscopy) and DC-magnetization confirms that the cluster size is in the sub-nanometric range and shows that the magnetization of the system is dramatically reduced, reaching only 38% of the bulk value. The experimental system has been reproduced by density functional theory calculations on Ni m clusters (m = 1-6, 10 and 13) embedded in Ag. The combination of the experimental and theoretical analysis points out that there is a breakdown of magnetism occurring below a cluster size of six atoms. According to our results, the loss of magnetic moment is not due to Ag-Ni hybridization but to charge transfer between the Ni sp and d orbitals, and the reduced magnetization observed experimentally is explained on the basis of the presence of a narrow cluster size-distribution where magnetic and non-magnetic clusters coexist.

3.
Sanid. mil ; 70(3): 197-203, jul.-sept. 2014. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-128440

RESUMO

Con diseño retrospectivo se analizan 4.452 pacientes con cáncer de vejiga, procedentes del Registro de Tumores de Madrid. La serie ha sido seguida durante 15 años. Se estudian las variables que dicho registro posee. La edad media ha sido de 68,7 (DT 11,95). La razón de sexo ha sido de 5:1. Se ha encontrado la siguiente histología: N. Uroteliales 94,3%, N. Células escawmosas 3,2%, N. glandulares 0,9% y otras histologías 0,5%. La extensión tumoral más frecuente se ha correspondido con la localizada con un 83,5%, seguida de la regional con un 10,6%, la diseminada con un 4,9% y por último los "in situ" con un 0,7%. Los tratamientos han sido: Cirugía 37,8%; Cirugía más radioterapia 6,2%; Cirugía más Quimioterapia 38,4%; Cirugía más Inmunoterapia 6% y paliativos 2,7%. Se ha encontrado un 4% de tumores primarios múltiples. Este estudio ha permitido conocer la realidad del cáncer vesical en todas sus vertientes por proceder los pacientes del día a día de la misma población en la que se diagnosticaron a los enfermos


Retrospective design with 4,452 patients with bladder cancer from the Cancer Registry of Madrid are analyzed. The series has been followed for 15 years. Variables such registration has studied. The mean age was 68.7 (SD 11.95). The sex ratio was 5:1. We found the following histology: N. urothelial 94.3%, N. squamous cells 3.2%, N. glandular histologies 0.9% and other 0.5%. The most frequent tumor extension has corresponded with localized with 83.5%, followed by 10.6% with regional, disseminated with 4.9% and finally the "in situ" with 0.7 %. The treatments were: surgery 37.8%; surgery and radiotherapy 6.2%; surgery and chemotherapy 38.4%; immunotherapy and surgery 6% and palliative 2.7%. Found 4% of multiple primary tumors. This study has shown the reality of bladder cancer in all its aspects by patients come daily from the same population in which the patients were diagnosed


Assuntos
Humanos , Neoplasias da Bexiga Urinária/epidemiologia , Carcinoma de Células de Transição/epidemiologia , Carcinoma de Células Escamosas/epidemiologia , Hospitais Públicos/estatística & dados numéricos , Estudos Epidemiológicos , Neoplasias Epiteliais e Glandulares/epidemiologia
4.
Sanid. mil ; 70(2): 95-97, abr.-jun. 2014. ilus
Artigo em Espanhol | IBECS | ID: ibc-125560

RESUMO

Las complicaciones vasculares secundarias al tratamiento quirúrgico del cáncer de riñón localizado son más frecuentes cuando se realiza una cirugía conservadora de nefronas. Sin embargo, la nefrectomía radical no está exenta de ellas. Presentamos el caso de un pseudoaneurisma de la arteria renal principal con comunicación fistulosa a colon ascendente tras nefrectomía radical. El paciente debutó con un episodio de shock hipovolémico debido a hemorragia digestiva baja


Vascular complications secondary to surgical treatment of localized kidney cancer are more frequent when a nephronsparing surgery is performed. However, radical nephrectomy is not exempt from them. We report the case of a pseudoaneurysm of the main renal artery with fistulous communication to ascending colon after radical nephrectomy. The patient presented with an episode of hypovolemic shock due to lower gastrointestinal bleeding


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Falso Aneurisma/complicações , Artéria Renal , Fístula Intestinal/complicações , Nefrectomia , Complicações Pós-Operatórias , Hemorragia Gastrointestinal/etiologia
5.
Transplant Proc ; 42(8): 2892-5, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20970562

RESUMO

INTRODUCTION: Vesicoureteral reflux (VUR), a complication after kidney transplantation, may be caused by recurrent urinary tract infections evaluating in life-threatening pyelonephritis and urosepsis. Open surgical correction is the standard treatment despite its morbidity. However, minimally invasive approaches are available. MATERIALS AND METHODS: Our study group describes seven patients with functioning kidney grafts and a diagnosis of VUR associated with recurrent urinary tract infections. The procedure was performed under antibiotic prophylaxis and spinal anesthesia. An endoscopic injection of 1 mL of biomaterial (copolymer of dextranomer and hyalurunic acid) was administered into the ureteral neo-orifice following the Sting technique. The catheter was removed within 24 hours in all cases. RESULTS: Between June 2009 and January 2010, nine procedures were performed in seven patients. Two patients experienced self-limiting post-surgical episodes of hematuria that did not need urologic manipulation. There were no episodes of retention, ureteral obstruction, or urinary infections. One patient required a reinjection 5 months later as a result of clinical failure. Apart from this one case, the other patients showed improvements with no infectious complications. CONCLUSIONS: Endoscopic correction with VUR seems to be a reliable and safe option as a first treatment for the transplant patient. More cases are required in order to improve the learning curve and, therefore, the success rate. Closer monitoring is needed to evaluate the efficiency of the copolymer, the evolution of the reflux, and the possible long-term complications in this sort of patients.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Transplante de Rim , Infecções Urinárias/terapia , Refluxo Vesicoureteral/terapia , Idoso , Feminino , Humanos , Masculino , Recidiva
6.
J Nanosci Nanotechnol ; 10(4): 2759-63, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20355497

RESUMO

The yttria partially stabilized zirconia is a very attractive material for orthopaedic applications. It exhibits excellent biocompatibility, high fracture toughness, high strength and low wear rates. But case studies show that delayed failure can occur in vivo due to crack propagation. Carbon nanotubes could avoid the slow crack propagation and enhance the toughness of the ceramic material used for prostheses fabrication. In this work, X-ray diffraction has been used to study the influence of the addition of MWCNT on the hydrothermal synthesis of tetragonal zirconia nanoparticles and on the phase stability of the CNT-nanozirconia nanocomposite with the temperature. First, the influence of the processing variables on the hydrothermal synthesis has been studied. The theoretical mathematical models that relate the percentage of tetragonal zirconia nanocrystals and the relative crystallinity with the processing variables in the range of analyzed values have been obtained. The values that give the maximum percentage and crystallinity of tetragonal phase in the studied range have been established. No significant differences were observed in the crystalline phases obtained when adding MWCNT during the synthesis. Nanozirconia partially coated MWCNT synthesized under the optimized parameters were added to commercially available nanozirconia particles and their influence in the phase stability of the zirconia with the temperature was studied by XRD. It was concluded that the addition of the carbon nanotubes delays both the monoclinic phase decomposition and the grain growth.

7.
Anim Reprod Sci ; 110(1-2): 79-95, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18243603

RESUMO

Holstein cows received, subcutaneously a non-degradable implant containing 5mg of the GnRH agonist Deslorelin (DESL) or no implant (CON) at 2+/-1 days postpartum (dpp). All cows were injected with PGF(2alpha) at 9 dpp. Previous pregnant (PPH) and non-pregnant uterine horns (PNPH) were determined by palpation per rectum. In Experiment 1, cows [DESL implant (n=10) and CON (n=9)] were examined by ultrasonography to record ovarian structures (23, 30 and 37 dpp) and uterine horn and cervical diameters (16, 23, 30 and 37 dpp). Uterine tone was scored before ultrasonography. Vaginoscopy was conducted just after ultrasonography examination to assess cervical discharge and color of the external cervical os. Blood samples were collected on a weekly basis for hormonal analyses. In Experiment 2, cows [DESL implant (n=77) and CON (n=70)] were palpated per rectum and vaginoscopy at 30 dpp for scoring of uterine tone, uterine horns, cervical diameter, and discharge. Blood samples were collected only at 9 dpp. In Experiment 1, DESL-implant-treated cows had more Class 1 follicles (P<0.01), less Class 2 (P<0.01) and Class 3 follicles (P<0.01) and no corpus luteum (CL) formation (P<0.01). In CON cows, six of nine animals had visible CL at 25+/-7 dpp. At 9 dpp plasma concentration of E(2), P(4) (P<0.01) and PGFM (P<0.05) were less in the DESL-implant treatment group. Diameter of PPH (P<0.01), PNPH (P<0.01) and cervix (P=0.08) were less in the DESL-implant treatment associated with greater uterine tone (P=0.07). The DESL-implant cows had a greater frequency of clear cervical discharge (P=0.09) and pink cervical os (P=0.06). In Experiment 2, plasma concentrations of PGFM were less at 9 dpp in DESL-implant treatment (P<0.01). Diameters of the PPH (P<0.01) and PNPH (P<0.01) were less and more uterine tone (P<0.01) in the DESL-implant treatment. Diameter of cervix and frequency of a cervical discharge score did not differ between treatments. Treatment with non-degradable Deslorelin (5mg) implant during postpartum: (1) suppressed ovarian follicular development, (2) enhanced physical involution of the uterus and cervix, (3) increased tone of the uterine wall, (4) decreased frequency of purulent cervical discharges, and (5) reduced inflammatory processes of the reproductive tract.


Assuntos
Bovinos/fisiologia , Folículo Ovariano/efeitos dos fármacos , Período Pós-Parto/efeitos dos fármacos , Pamoato de Triptorrelina/análogos & derivados , Útero/efeitos dos fármacos , Animais , Bovinos/sangue , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Implantes de Medicamento , Estradiol/sangue , Feminino , Hormônio Liberador de Gonadotropina/agonistas , Análise Multivariada , Folículo Ovariano/fisiologia , Período Pós-Parto/sangue , Progesterona/sangue , Distribuição Aleatória , Estatísticas não Paramétricas , Pamoato de Triptorrelina/administração & dosagem , Útero/fisiologia
8.
Anim Reprod Sci ; 111(2-4): 320-37, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18455332

RESUMO

The objective was to evaluate pregnancy rate to a timed artificial insemination (TAI) protocol in the autumn for cows treated with a non-degradable GnRH agonist implant (Deslorelin [DESL], 5mg) during the summer heat stress period compared with non-treated controls (CON). Cows were randomly assigned to receive or not a DESL implant within 1-4 days postpartum (dpp) twice weekly, from 25 June through 8 August 2001. All cows in DESL implant and CON treatments were injected with PGF(2alpha) 7 days after enrollment. Ultrasonography (US) monitored numbers of ovarian follicles and corpus luteum (CL) at approximately 10, 30, 35/36, 45/44, 56/55 and 66/63dpp, while DESL implants were in situ and concurrently CON, respectively. DESL implants were removed at two specific days, 28 August and 4 September. Cows had DESL implant in situ for a range of 28-67 days, depending on date of enrollment and implant removal. Within 61-100dpp, 31 days after implant removal, DESL implant and CON cows were initiated in a Presynch-Ovsynch and TAI protocol. Pregnancy was evaluated by US and palpation per rectum at 28 and 46 days after TAI, respectively. Plasma concentrations of progesterone were analyzed for sets of blood samples collected during the Presynch-Ovsynch and at TAI day followed 8 days later. Cows in the DESL-implant treatment had more (P<0.01) Class 1 (3-5mm) follicles, less (P<0.01) Class 2 (6-9mm), Class 3 (> or =10mm) follicles and CL compared with CON cows. Proportion of cows having initiated estrous cycles after calving was less (P<0.01) in the DESL-implant treatment (52.2%, 58/111) compared with CON (93.7%, 104/111) at the beginning of Ovsynch. Pregnancy rate to TAI was less (P<0.01) in the DESL implant (27.5%, 33/120) compared with CON (53.9%, 69/128). Pregnancy rate to TAI was less (P<0.01) in DESL-implanted cows that had initiated estrous cycles after calving (30.6%, 19/62) compared with CON (53.7%, 65/121) cows having initiated estrous cycles after calving. Furthermore, pregnancy rate was less (P<0.01) for cows having ovulations that had initiated estrous cycles after TAI in the DESL implant (39.1%, 18/46) compared with CON (62.1%, 54/87) treatments. Pregnancy losses from day 28 to day 46 of pregnancy did not differ between DESL implant (15.1%, 5/33) and CON (13.0%, 9/69) treatments. The DESL implant induced a delay in initiation of a new wave of follicular development during the postpartum-heat stressed period. The lesser pregnancy rate in the DESL-implant treatment group may be due to a pool of heat stress damaged follicles that were depleted in the control group with re-occurring follicle waves.


Assuntos
Bovinos/fisiologia , Inseminação Artificial/veterinária , Folículo Ovariano/efeitos dos fármacos , Reprodução/efeitos dos fármacos , Pamoato de Triptorrelina/análogos & derivados , Animais , Implantes de Medicamento , Sincronização do Estro , Feminino , Inseminação Artificial/métodos , Lactação , Modelos Logísticos , Folículo Ovariano/diagnóstico por imagem , Período Pós-Parto , Gravidez , Progesterona/sangue , Distribuição Aleatória , Pamoato de Triptorrelina/administração & dosagem , Ultrassonografia
9.
Theriogenology ; 65(8): 1443-53, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16219343

RESUMO

Embryonic and fetal mortality reduce reproductive performance of lactating dairy cows. The objectives of this study were to reduce pregnancy loss by administering a deslorelin implant (GnRH agonist) during the late embryonic period, to reduce follicular growth, induce accessory corpora lutea, and increase plasma progesterone concentrations. Lactating dairy cows received an implant containing 2.1 mg of deslorelin (Deslorelin group; n = 89) or no treatment (Control group; n = 92) on Day 27 of pregnancy. Pregnancy, ovarian structures and plasma progesterone concentrations were determined on Days 27 and 45, and pregnancy was re-confirmed on Day 90. On Day 45, mean +/- S.E.M. numbers of class 2 (6-9 mm; 0.72+/-0.19) and class 3 (> or = 10 mm; 0.86 +/- 0.12) follicles for cows in the Deslorelin group were lower (P < 0.01) than the numbers of class 2 (1.90 +/- 0.18) and class 3 (1.92 +/- 0.12) follicles for cows in the Control group. On Day 45, the number of accessory corpora lutea for cows in the Deslorelin group (1.80 +/- 0.07) were greater (P < 0.01) than for cows in the Control group (1.31 +/- 0.07). On Day 45, plasma progesterone concentration was increased (P < 0.01) for cows in the Deslorelin group (8.03 +/- 0.33 ng/mL) compared to cows in the Control group (6.40 +/- 0.31 ng/mL). Pregnancy losses did not differ between Days 27 and 45 and Days 45 and 90 for cows in the Control (15.2 and 11.0%, respectively) and Deslorelin groups (20.2 and 10.5%, respectively). However, in the Deslorelin group, pregnancy loss between Days 45 and 90 was lower (P < 0.05) for cows that formed an accessory CL (0%) compared to cows that did not form an accessory CL (16.1%).


Assuntos
Aborto Animal/epidemiologia , Bovinos/fisiologia , Fármacos para a Fertilidade Feminina/farmacologia , Prenhez/efeitos dos fármacos , Progesterona/sangue , Pamoato de Triptorrelina/análogos & derivados , Aborto Animal/prevenção & controle , Animais , Corpo Lúteo/efeitos dos fármacos , Corpo Lúteo/fisiologia , Implantes de Medicamento , Feminino , Fármacos para a Fertilidade Feminina/administração & dosagem , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/fisiologia , Gravidez , Prenhez/sangue , Prenhez/fisiologia , Distribuição Aleatória , Reprodução/efeitos dos fármacos , Reprodução/fisiologia , Pamoato de Triptorrelina/administração & dosagem , Pamoato de Triptorrelina/farmacologia
10.
Theriogenology ; 63(6): 1617-27, 2005 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-15763106

RESUMO

The objective was to compare pregnancy rates and pregnancy losses in lactating dairy cows that were diagnosed not pregnant and re-inseminated following either the Ovsynch or Heatsynch protocols. Also evaluated were the effects of stages of the estrous cycle, ovarian cysts and anestrus on pregnancy rates for both treatments. Non-pregnant cows (n = 332) as determined by ultrasonography on day 27 post-AI (study day 0) were divided into two groups. Cows in the Ovsynch group (n = 166) received GnRH on day 0, PGF2alpha on day 7, GnRH on day 9, and timed AI (TAI) 16 h later (day 10). Cows in the Heatsynch group (n = 166) received GnRH on day 0, PGF2alpha on day 7, estradiol cypionate (ECP) on day 8, and TAI 48 h later (day 10). Cows detected in estrus on days 8 and 9 were inseminated and included in the study. On day 0, cows were classified according to different stages of the estrous cycle, or presence of ovarian cysts or anestrus. Pregnancy rates were evaluated 27, 45 and 90 days after resynchronized AI. Overall, there was no difference in pregnancy rates on days 27, 45 and 90 between cows in the Ovsynch (25.2, 17.5, and 13.9%) and Heatsynch (25.8, 19.9, and 16.1%) groups. There was no difference in pregnancy losses from days 27 to 45 and days 45 to 90 for cows in the Ovsynch (25.0 and 17.9%) and Heatsynch (14.7 and 10.3%) groups. However, pregnancy rates were increased when cows in metestrus were subjected to the Heatsynch protocol and cows with ovarian cysts were subjected to the Ovsynch protocol.


Assuntos
Bovinos/fisiologia , Estradiol/análogos & derivados , Inseminação Artificial/veterinária , Indução da Ovulação/veterinária , Ultrassonografia/veterinária , Animais , Dinoprosta/administração & dosagem , Estradiol/administração & dosagem , Ciclo Estral , Sincronização do Estro , Feminino , Hormônio Liberador de Gonadotropina/administração & dosagem , Inseminação Artificial/métodos , Lactação , Indução da Ovulação/métodos , Gravidez , Fatores de Tempo
11.
Theriogenology ; 63(6): 1628-42, 2005 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-15763107

RESUMO

Pregnancy rates were compared in lactating dairy cows (n = 1083) assigned to protocols for resynchronization of ovulation based on stages of the estrous cycle, or presence of ovarian cysts or anestrus. Cows were detected not pregnant by ultrasonography 30 d after a previous AI (study day 0) and classified as diestrus, metestrus, proestrus, with ovarian cysts or anestrus. Cows in diestrus (January-May) were assigned to either Ovsynch (GnRH day 0, PGF2alpha day 7, GnRH day 9, and timed-AI [TAI] 16 h later; n = 96), or Quicksynch (PGF2alpha day 0, estradiol cypionate [ECP] day 1, AI at detected estrus [AIDE] on day 2, or TAI on day 3; n = 96). Cows in diestrus (June-December) were assigned to either Ovsynch (n = 156) or Modified Quicksynch (PGF2alpha day 0, ECP day 1, AIDE days 2 and 3, and to Ovsynch on day 4 if not detected in estrus; n = 142). Cows in metestrus were assigned either to Ovsynch (n = 68), Heatsynch (GnRH day 0, PGF2alpha day 7, ECP day 8, AIDE day 9, or TAI day 10; n = 62), or GnRH + Ovsynch (GnRH on day 0, followed by Ovsynch on day 8; n = 64). Cows in proestrus, with ovarian cysts, or anestrus were assigned to either Ovsynch (proestrus n = 89, ovarian cysts n = 97, anestrus n = 8) or GnRH + Ovsynch (proestrus n = 87, ovarian cysts n = 109, anestrus n = 9). Pregnancy rate was evaluated 30, 55 and 90 d after resynchronized AI. For cows in diestrus (January-May), pregnancy rates were higher for Ovsynch (35.9, 29.2 and 26.0%) than for Quicksynch (21.7, 16.7 and 15.6%). For cows in diestrus (June-December), pregnancy rates were similar for Ovsynch (34.4, 24.0 and 23.6%) and Modified Quicksynch (27.1, 26.2 and 21.6%). For cows in metestrus, pregnancy rates were higher for GnRH + Ovsynch (33.3, 24.5 and 20.3%) than for Heatsynch (20.3, 12.9 and 9.8%). For cows with ovarian cysts, pregnancy rates were higher for GnRH + Ovsynch (30.3, 26.6 and 22.9%) than for Ovsynch (20.2, 18.5 and 14.7%). Assignment to resynchronization protocols based on the stages of the estrous cycle, or presence of ovarian cysts improved pregnancy rates.


Assuntos
Anestro , Doenças dos Bovinos , Estradiol/análogos & derivados , Ciclo Estral , Inseminação Artificial/veterinária , Cistos Ovarianos/veterinária , Indução da Ovulação/veterinária , Animais , Bovinos , Diestro , Dinoprosta/administração & dosagem , Estradiol/administração & dosagem , Sincronização do Estro , Feminino , Hormônio Liberador de Gonadotropina/administração & dosagem , Inseminação Artificial/métodos , Lactação , Metestro , Indução da Ovulação/métodos , Gravidez , Proestro , Estações do Ano , Fatores de Tempo , Ultrassonografia/veterinária
12.
Theriogenology ; 63(4): 1026-37, 2005 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-15710190

RESUMO

The objective of this study was to determine the effect of GnRH (100 microg i.m.) treatment 5 and 15 days after timed insemination (TAI) on pregnancy rate and pregnancy loss in lactating dairy cows subjected to synchronization of ovulation. The study included 831 lactating dairy cows subjected to a Presynch-Ovsynch protocol for first service. On the day of TAI (Day 0), cows were randomly assigned to one of four experimental groups. Cows in Group 1 (n = 214) were treated with GnRH on Day 5; cows in Group 2 (n = 209) were treated with GnRH on Day 15; cows in Group 3 (n = 212) were treated with GnRH on both Day 5 and Day 15; cows in Group 4 (n = 196) were not treated. Pregnancy rate was evaluated at Day 27 and Day 45 after TAI. The interestrus interval and the proportion of cows diagnosed not pregnant based on expression of estrus and insemination before pregnancy diagnosis on Day 27 were determined. The results of this study are: (1) GnRH treatment on Day 5 or Day 15 did not increase pregnancy rate, or reduce pregnancy loss between Day 27 and Day 55 after TAI; (2) cows treated with GnRH on both Day 5 and Day 15 had a lower (P < 0.01) proportion of cows diagnosed not pregnant based on expression of estrus before ultrasonography on Day 27 (26.5%) compared to control cows (52.9%), and these cows had an extended (P = 0.05) interestrus interval (23.4 days vs. 21.5 days); and (3) GnRH treatment on both Day 5 and Day 15 after TAI reduced pregnancy rate on Day 27 (36.8% vs. 44.4% for control cows; P < 0.03) and Day 55 (28.3% vs. 36.2% for control cows; P < 0.01). Therefore, strategies to stimulate CL function using multiple doses of GnRH during the luteal phase need to consider potential negative effects.


Assuntos
Aborto Animal/prevenção & controle , Bovinos/fisiologia , Hormônio Liberador de Gonadotropina/administração & dosagem , Inseminação Artificial/veterinária , Lactação , Indução da Ovulação/veterinária , Análise de Variância , Animais , Feminino , Inseminação Artificial/métodos , Razão de Chances , Gravidez , Estações do Ano , Fatores de Tempo , Ultrassonografia/veterinária
13.
J Dairy Sci ; 87(4): 921-32, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15259226

RESUMO

The objectives were to determine the effect of dietary fish oil (FO) on uterine secretion of PGF2alpha, milk production, milk composition, and metabolic status during the periparturient period. Holstein cows were assigned randomly to diets containing FO (n = 13) or olive oil (OO, n = 13). Cows were fed prepartum and postpartum diets that provided approximately 200 g/d from 21 d before the expected parturition until 21 d after parturition. The FO used contained 36% eicosapentaenoic acid (EPA, C20:5, n-3) and 28% docosahexaenoic acid (DHA, C22:6, n-3). Blood samples were obtained from 14 d before the due date until d 21 postpartum. A total of 6 FO and 8 OO cows without periparturient disorders were used in the statistical analyses of PGF2alpha-metabolite (PGFM) and metabolite concentrations. Length of prepartum feeding with OO or FO did not differ. Proportions of individual and total n-3 fatty acids were increased in caruncular tissue and milk of cows fed FO. The combined concentrations of EPA and DHA in caruncular tissue were correlated positively with the number of days supplemented with FO. Cows fed FO had reduced concentrations of plasma PGFM during the 60 h immediately after parturition compared with cows fed OO. Concentrations of prostaglandin H synthase-2 mRNA and protein in caruncular tissue were unaffected by diet. Production of milk and FCM were similar between cows fed the two oil diets. However, cows fed FO produced less milk fat. Feeding FO reduced plasma concentrations of glucose. Dietary fatty acids given during the periparturient period can reduce the uterine secretion of PGF2alpha in lactating dairy cows and alter the fatty acid profile of milk fat.


Assuntos
Bovinos/fisiologia , Gorduras Insaturadas na Dieta/administração & dosagem , Dinoprosta/análogos & derivados , Dinoprosta/metabolismo , Óleos de Peixe/administração & dosagem , Leite/química , Útero/metabolismo , Animais , Ciclo-Oxigenase 2 , Dinoprosta/análise , Dinoprosta/sangue , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análise , Ácidos Graxos/análise , Ácidos Graxos Ômega-3/análise , Feminino , Isoenzimas/análise , Isoenzimas/genética , Lactação , Lipídeos/análise , Azeite de Oliva , Parto , Placenta/química , Óleos de Plantas/administração & dosagem , Gravidez , Prostaglandina-Endoperóxido Sintases/análise , Prostaglandina-Endoperóxido Sintases/genética , RNA Mensageiro/análise
14.
Theriogenology ; 61(2-3): 407-19, 2004 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-14662140

RESUMO

The objective of this study was to evaluate ovarian function after inducing ovulation with a deslorelin implant in nonlactating dairy cows and heifers. Cattle received GnRH on Day -9, and PGF2alpha on Day -2. On Day 0, in Experiment 1, cows received either 100 microg GnRH (Control), a 750 microg (DESLORELIN 750) or 1000 microg (DESLORELIN 1000) deslorelin implant. On Day 0, in Experiment 2, cows received 100 microg of GnRH or a 450 microg (DESLORELIN 450) deslorelin implant. In Experiments 1 and 2, cows received PGF2alpha on Day 16. Ultrasonography and blood sampling for plasma progesterone (P4) were used to monitor ovarian activity. On Day 0, in Experiment 3, heifers received either 100 microg of GnRH or 750 microg (DESLORELIN 750) deslorelin implant. On Day 16, all heifers received PGF2alpha. Blood samples were collected on Days 7, 13 and 16. In Experiments 1-3, deslorelin implants did not elevate plasma concentrations of P4 in a systematic manner during the late luteal phase. In Experiments 1 and 2, deslorelin implants decreased the size of the largest follicle and the number of Class II and III follicles. In Experiments 1 and 2, deslorelin-treated cows failed to ovulate by Day 28. In conclusion, deslorelin implants induced ovulation, stimulated development of a normal CL, and delayed follicular growth during the subsequent diestrus period. For future applications, the dose of the deslorelin implant will have to be adjusted, and if used for timed-inseminations, nonpregnant cows will have to be resynchronized to minimize delayed returns to estrus and ovulation.


Assuntos
Bovinos/fisiologia , Indução da Ovulação/veterinária , Pamoato de Triptorrelina/análogos & derivados , Pamoato de Triptorrelina/administração & dosagem , Animais , Corpo Lúteo/efeitos dos fármacos , Corpo Lúteo/fisiologia , Dinoprosta/administração & dosagem , Implantes de Medicamento , Feminino , Hormônio Liberador de Gonadotropina/administração & dosagem , Folículo Ovariano/anatomia & histologia , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/fisiologia , Ovário/diagnóstico por imagem , Indução da Ovulação/métodos , Progesterona/sangue , Ultrassonografia
15.
Am J Emerg Med ; 20(5): 453-62, 2002 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12216044

RESUMO

This study was performed to compare the effectiveness of external thoracic compressions with and without intra-aortic occlusion balloon with capnography and coronary and cerebral perfusion pressure (CPP) in the normothermic and traumatic-less cardiopulmonary arrest provoked by a ventricular fibrillation in pigs. This was an experimental study (cross-over study) in 14 pigs with similar characteristics (23 +/- 2 kg, 10-12 weeks of age). After an 8-minute nonintervention period, the cardiopulmonary resuscitation (CPR) consists of 4 periods of 5 minutes alternating CPR with and without intra-aortic occlusion balloon. Main outcomes measured are end-tidal CO(2) (ETCO(2)); intra-aortic, coronary, and cerebral perfusion pressures; blood gas analysis; and blood lactate concentration. At the end of each period, levels are obtained. Postmortem study was made. Inflation of the occlusion balloon provokes an expansion in the ETCO(2) of about 38%. The coronary perfusion pressure initially goes from 10.21 to 29.0 mm Hg after the occlusion of the aorta, which means an increase of 150%. The CPP goes from 12.54 to 39.71 mm Hg after the balloon was inflated, which means an increase of 200%. In all cases the differences are statistically significant (P <.0001). These increases are less important in the final periods. Intra-aortic balloon occlusion increased ETCO(2), coronary, and cerebral perfusion pressures. An early application of this technique was important.


Assuntos
Débito Cardíaco , Reanimação Cardiopulmonar/métodos , Modelos Animais de Doenças , Parada Cardíaca/terapia , Massagem Cardíaca/métodos , Balão Intra-Aórtico/métodos , Animais , Gasometria , Testes Respiratórios , Capnografia , Dióxido de Carbono/análise , Reanimação Cardiopulmonar/normas , Estudos Cross-Over , Parada Cardíaca/etiologia , Parada Cardíaca/metabolismo , Parada Cardíaca/fisiopatologia , Massagem Cardíaca/normas , Hemodinâmica , Balão Intra-Aórtico/normas , Ácido Láctico/sangue , Distribuição Aleatória , Suínos , Volume de Ventilação Pulmonar , Fatores de Tempo , Resultado do Tratamento , Fibrilação Ventricular/complicações
16.
Rev. chil. obstet. ginecol ; 51(1): 61-7, 1986. tab
Artigo em Espanhol | LILACS | ID: lil-44560

RESUMO

Se analizó una población de 117 parejas infértiles, sometidas a una metodología de estudio basada en la prueba poscoital. La metodología empleada se muestra bastante eficaz en las parejas con prueba poscoital satisfactoria, puesto que significa, gran ahorro de exámenes y de tiempo de estudioo. Si bien no descarta al varón, en un alto porcentaje (80%) éste no participó como factor etiológico. La presencia de factor masculino se acompañó prácticamente en la totalidad de las veces de una prueba poscoital insatisfactoria: por lo tanto, en estas parejas debe realizarse, como acto siguiente, de estudio de espermiograma. 46% de las parejas infértiles, aquéllas con prueba poscoital satisfactoria, se benefician con esta metodología, pudiendo efectuar tratamientos adecuados, sin necesidad de gran tecnología y con ahorro importante de tiempo de estudioo


Assuntos
Adulto , Humanos , Masculino , Feminino , Muco do Colo Uterino/citologia , Infertilidade/diagnóstico , Endométrio/patologia , Infertilidade/etiologia , Contagem de Espermatozoides , Motilidade dos Espermatozoides
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