Acceleration of Angiogenesis after Paravasal Injection of Mesenchymal Stem Cells at the Site of Modeled Venous Thrombosis.

The results of transplantation of autologous bone marrow multipotent mesenchymal stem cells carrying GFP gene and labeled with cell nucleus-specific dye DAPI near the thrombosed vein in rat hind limb were studied by methods of luminescent microscopy. It was demonstrated that autologous multipotent mesenchymal stem cells participate in the formation of granulations at the site of surgery. The blood fl ow in the thrombosed great vein was always restored through thrombolysis. We observed no signs of incorporation of the transplanted cells into the wall of the great vessel, clot recanalization, or formation of collaterals. Small branches of the great vein in the affected region were also thrombosed. The blood fl ow in these branches was always restored with participation of the transplanted cells or through clot recanalization or through obliteration of the thrombosed vessels and formation of new vessels. The transplanted cells and structures formed by them were gradually replaced by the recipient cells.

[Morphological results of stromal stem cells of bone marrow origin into the thrombosed vein in experiment].

Using the methods of luminescent microscopy, the results of injection of autologous multipotent stromal (mesenchymal) stem cells of bone marrow origin (SSCBMO) containing GFP gene, into thrombosed hindlimb vein were studied in 226 male Wag rats. It was found that the restoration of blood flow through the thrombosed main vein was not always the result of thrombolysis. No signs of incorporation of injected SSCBMO into the wall of thrombosed vessel, clot recanalization or collateral formation were detected. In experimental thrombosis model with thrombin administration and main vein ligation, the thrombosis of its small branches also took place. The restoration of blood flow occured via either blood clot recanalization or obliteration of thrombosed vessels and the outgrowth of the new ones. SSCBMO were found to participate in both of these processes resulting in faster restoration of a blood flow in the tissue microregion of thrombosed vein. Gradually the injected SSCBMO and the structures formed with their participation, were replaced by the own cells of a recipient organism.

[A study of neutralizing activity and cross-reactivity of monoclonal antibodies to ectromelia virus with orthopoxviruses pathogenic for man]. / Neitralizuiushchaia aktivnost' i perekrestnoe reagirovanie monoklonal'nykh antitel k virusu éktromelii s patogennymi dlia cheloveka ortopoksvirusami.

An extensive collection of 125 rat hybridomas secreting monoclonal antibodies (Mabs) to ectromelia virus (EV) polypeptide (Poxviridae family, Orhtopoxvirus genus) was set up. A significant portion of Mabs (37 types) recognized epitopes of the 14 kDa polypeptide as well as the 37 and 35 kDa polypeptides. However, a majority of Mabs interacted with conformation-dependent epitopes, which were destroyed in immunoprecipitation. One hundred and thirteen of Mabs cross-interacted with antigenic determinants of vaccinia viruses (VV), cowpox virus (CPV) and smallpox virus (SPV); only 12 of them were found to be specific to EV. The Mabs antigenic activity was tested for 46 types of cross-reactivity Mabs in VV neutralization on Vero cells. Only the 112H12, 113D5, 113F8, 122H9 and 125G9 Mabs, which were specific to the kDa 14 polypeptide (gene A30L EV), had the neutralizing activity. The 122H9 and 125G9 Mabs were able to neutralize SPV. Therefore, it can be assumed that the 14 kDa polypeptide carries, on its surface, cross-reactivity neutralizing epitopes typical of orthopoxviruses.