RESUMO
In Wistar rats, specific inhibitor UPS lactacystin induced degeneration of 24% of the dopaminergic neurons in the black substance. The work shows that a moderate weakening of the UPS function is characterized by an enhanced activity of the shaperon system. This process seems to restore and maintain population of the dopaminergic neurons.
Assuntos
Proteínas de Choque Térmico HSP70/biossíntese , Neurônios/efeitos dos fármacos , Doença de Parkinson , Complexo de Endopeptidases do Proteassoma/efeitos dos fármacos , Substância Negra/patologia , Tirosina 3-Mono-Oxigenase/biossíntese , Acetilcisteína/análogos & derivados , Acetilcisteína/farmacologia , Envelhecimento/fisiologia , Animais , Proteínas de Bactérias/análise , Biotina/análogos & derivados , Biotina/análise , Western Blotting , Cateterismo , Inibidores de Cisteína Proteinase/farmacologia , Modelos Animais de Doenças , Dopamina/farmacologia , Humanos , Imuno-Histoquímica , Microinjeções , Microscopia Confocal , Neurônios/citologia , Doença de Parkinson/patologia , Doença de Parkinson/fisiopatologia , Complexo de Endopeptidases do Proteassoma/metabolismo , Inibidores de Proteassoma , Ratos , Ratos Wistar , Substância Negra/efeitos dos fármacos , Substância Negra/fisiopatologia , Ubiquitina/antagonistas & inibidores , Ubiquitina/metabolismo , Regulação para CimaRESUMO
The objective of this work was to perform a parallel analysis of activation of the rat anterior hypothalamus cells as judged by c-Fos protein expression, and of the expression of interleukin-2 (IL-2) under different influences, i. e., mild stress (handling) and adaptation to it, and intranasal administration of saline and the peptides Vilon (Lys-Glu) and Epithalon (Ala-Glu-Asp-Gly). Changes in the counts of cells positive for c-Fos- and IL-2 proteins were studied in structures of the lateral (LHA) area, anterior (AHN), supraoptic (SO) and paraventricular (PVH) nuclei of Wistar rat hypothalamus. Quantity of the interleukin-2-positive and c-Fos-positive cells was calculated. The findings were: a negative correlation between the activation of cells and the amount of IL-2 in the cells in the hypothalamic structures under study, and the specific patterns of changes in the counts of cells positive for c-Fos and IL-2 under stress and adaptation to stress.
