RESUMO
Tn502mer differs in size and restriction map from the well-characterized Tn501mer. It also differs in its preferential and high-frequency insertion into the 6 kb PstI-C region of RP1. The affinity for this region is perpetuated in pVS76, a clone of RP1 PstI-C in pBR322. Restriction mapping of independent pVS76::Tn502 derivatives revealed that Tn502 inserted at the same site (or small region) in PstI-C corresponding to the 35 kb coordinate in RP1. Insertion occurred in both orientations, but one was preferred. When PstI-C was deleted from RP1, acquisition of Tn502 was reduced and the sites of insertion randomized.
Assuntos
Elementos de DNA Transponíveis , Mercúrio/farmacologia , Pseudomonas aeruginosa/genética , Sítios de Ligação , Conjugação Genética , Resistência Microbiana a Medicamentos/genética , Plasmídeos , Pseudomonas aeruginosa/efeitos dos fármacos , Mapeamento por RestriçãoRESUMO
The perceptual complexity of lexically ambiguous and unambiguous sentences was compared in three experiments. In Experiment 1, the report of ambiguous words from rapidly presented ambiguous sentences was worse than the report of corresponding unambiguous words from unambiguous sentences. Results of Experiment 2 showed that the effect was not reduced by the presence of prior biasing context within the sentence. Experiment 3 repeated the finding with a sentence meaning classification task. It was concluded that both meanings of a lexically ambiguous sentence must be computed, even when prior context makes one meaning more plausible than the other.
