The effect of anaerobic digestate as an organic soil fertilizer on the diversity and structure of the indigenous soil microbial and nematode communities.

Anaerobic digestate is a popular soil additive which can promote sustainability and transition toward a circular economy. This study addresses how anaerobic digestate modifies soil health when combined with a common chemical fertilizer. Attention was given to soil microbes and, a neglected but of paramount importance soil taxonomic group, soil nematodes. A mesocosm experiment was set up in order to assess the soil's microbial and nematode community. The results demonstrated that the microbial diversity was not affected by the different fertilization regimes, although species richness increased after digestate and mixed fertilization. The composition and abundance of nematode community did not respond to any treatment. Mixed fertilization notably increased potassium (K) and boron (B) levels, while nitrate (NO3-) levels were uniformly elevated across fertilized soils, despite variations in nitrogen input. Network analysis revealed that chemical fertilization led to a densely interconnected network with mainly mutualistic relationships which could cause ecosystem disruption, while digestate application formed a more complex community based on bacterial interactions. However, the combination of both orchestrated a more balanced and less complex community structure, which is more resilient to random disturbances, but on the downside, it is more likely to collapse under targeted perturbations.

Screening of pesticides and emerging contaminants in eighteen Greek lakes by using target and non-target HRMS approaches: Occurrence and ecological risk assessment.

Lakes, albeit ecosystems of vital importance, are insufficiently investigated with respect to the degradation of water quality due to the organic micropollutants load. As regards Greece, screening of lake waters is scarce and concerns a limited number of contaminants. However, understanding the occurrence of contaminants of emerging concern (CECs) and other micropollutants in lakes is essential to appraise their potential ecotoxicological effects. The aim of this study was to deploy a multiresidue screening approach based on liquid chromatography-high-resolution mass spectrometry (HRMS) to get a first snapshot for >470 target CECs, including pesticides, pharmaceuticals, personal care products (PPCPs), per- and polyfluoroalkyl substances (PFASs), as well as organophosphate flame retardants (OPFRs) in eighteen Greek lakes in Central, Northern and West Northern Greece. The omnipresent compounds were DEET (N,N-diethyl-meta-toluamide), caffeine and TCPP (tris (1-chloro-2-propyl) phosphate). Maximum concentrations varied among the different classes. DEET was detected at a maximum average concentration of >1000 ng/L in Lake Orestiada, while its mean concentration was estimated at 233 ng/L. The maximum total concentrations for pesticides, PPCPs, PFASs, and OPFRs were 5807, 2669, 33.1, and 1214 ng/L, respectively, indicating that Greek lakes are still threatened by the intense agricultural activity. Besides, HRMS enabled a non-target screening by exploiting the rich content of the full-scan raw data, allowing the 'discovery' of tentative candidates, such as surfactants, pharmaceuticals, and preservatives among others, without reference standards. The potential ecotoxicity was assessed by both the risk quotient method and ECOSAR (Ecological Structure Activity Relationships) revealing low risk for most of the compounds.

The Role of Microbial Inoculants on Plant Protection, Growth Stimulation, and Crop Productivity of the Olive Tree (Olea europea L.).

The olive tree (Olea europaea L.) is an emblematic, long-living fruit tree species of profound economic and environmental importance. This study is a literature review of articles published during the last 10 years about the role of beneficial microbes [Arbuscular Mycorrhizal Fungi (AMF), Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promoting Fungi (PGPF), and Endophytes] on olive tree plant growth and productivity, pathogen control, and alleviation from abiotic stress. The majority of the studies examined the AMF effect using mostly Rhizophagus irregularis and Glomus mosseae species. These AMF species stimulate the root growth improving the resistance of olive plants to environmental and transplantation stresses. Among the PGPR, the nitrogen-fixing bacteria Azospirillum sp. and potassium- and phosphorous-solubilizing Bacillus sp. species were studied extensively. These PGPR species were combined with proper cultural practices and improved considerably olive plant's growth. The endophytic bacterial species Pseudomonas fluorescens and Bacillus sp., as well as the fungal species Trichoderma sp. were identified as the most effective biocontrol agents against olive tree diseases (e.g., Verticillium wilt, root rot, and anthracnose).

Water quality in irrigation and drainage networks of Thessaloniki plain in Greece related to land use, water management, and agroecosystem protection.

A representative agricultural area of 150 ha located in a protected ecosystem (Axios River Delta, Thermaikos Gulf-N. Aegean, Greece) was selected in order to investigate water quality parameters [pH, electrical conductivity (EC(w)), NO(3)-N, NH(4)-N, total phosphorus (TP)] in irrigation and drainage water. In the study area, the cultivated crops are mainly rice, maize, cotton, and fodder. Surface irrigation methods are applied using open channels network, and irrigation water is supplied by Axios River, which is facing pollution problems. The return flow from surface runoff and the surplus of irrigation water are collected to drainage network and disposed to Thermaikos Gulf. A 2-year study (2006-2007) was conducted in order to evaluate the effects of land use and irrigation water management on the drainage water quality. The average pH and NO(3)-N concentration was higher in the irrigation water (8.0 and 1.3 mg/L, respectively) than that in the drainage water (7.6 and 1.0 mg/L, respectively). The average EC(W), NH(4)-N, and TP concentration was higher in the drainage water (1,754 muS/cm, 90.3 microg/L, and 0.2 mg/L, respectively) than that in the irrigation water (477.1 muS/cm, 46.7 microg/L, and 0.1 mg/L, respectively). Average irrigation efficiency was estimated at 47% and 51% in 2006 and 2007 growing seasons (April-October), respectively. The loads of NO(3)-N in both seasons were higher in the irrigation water (35.1 kg/ha in 2006 and 24.9 kg/ha in 2007) than those in the drainage water (8.1 kg/ha in 2006 and 7.6 kg/ha in 2007). The load of TP was higher in the irrigation water in season 2006 (2.8 kg/ha) than that in the drainage water (1.1 kg/ha). Total phosphorus load in 2007 was equal in irrigation and drainage water (1.2 kg/ha). Wetland conditions, due to rice irrigation regime, drainage network characteristics, and the crop distribution in the study area, affect the drainage water ending in the protected ecosystem of Thermaikos Gulf.