Targeted delivery of interleukin-12 plasmid into HepG2 cells through folic acid conjugated graphene oxide nanocarrier.

Successful gene therapy relies on carriers to transfer genetic materials with high efficiency and low toxicity in a targeted manner. To enhance targeted cell binding and uptake, we developed and synthesized a new gene delivery vector based on graphene oxide (GO) modified by branched polyethyleneimine (BPEI) and folic acid (FA). The GO-PEI-FA nanocarriers exhibit lower toxicity compared to unmodified PEI, as well as having the potential to efficiently condense and protect pDNA. Interestingly, increasing the polymer content in the polyplex formulation improved plasmid transfer ability. Substituting graphene oxide for PEI at an N/P ratio of 10 in the HepG2 and THP1 cell lines improved hIL-12 expression by up to approximately eightfold compared to simple PEI, which is twice as high as GO-PEI-FA in Hek293 at the same N/P ratio. Therefore, the GO-PEI-FA described in this study may serve as a targeting nanocarrier for the delivery of the hIL-12 plasmid into cells overexpressing folic acid receptors, such as those found in hepatocellular carcinoma.

A new tannin-based adsorbent synthesized for rapid and selective recovery of palladium and gold: Optimization using central composite design.

A tannin-based adsorbent was synthesized by pomegranate peel tannin powder modified with ethylenediamine (PT-ED) for the rapid and selective recovery of palladium and gold. To characterize PT-ED, field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS-Mapping), and Fourier transform infrared spectroscopy (FT-IR) were used. Central composite design (CCD) was used for optimization. The kinetic, isotherm, interference of coexisting metal ions, and thermodynamics were studied. The optimal conditions, including Au (III) concentration = 30 mgL-1, Pd (II) concentration = 30 mgL-1, adsorbent mass = 26 mg, pH = 2, and time = 26 min with the sorption percent more than 99 %, were anticipated for both metals using CCD. Freundlich model and pseudo-second-order expressed the isotherm and kinetic adsorption of the both metals. The inhomogeneity of the adsorbent surface and the multi-layer adsorption of gold and palladium ions on the PT-ED surface are depicted by the Freundlich model. The thermodynamic investigation showed that Pd2+ and Au3+ ions adsorption via PT-ED was an endothermic, spontaneous, and feasible process. The maximum adsorption capacity of Pd2+ and Au3+ ions on PT-ED was 261.189 mgg-1 and 220.277 mgg-1, respectively. The probable adsorption mechanism of Pd2+ and Au3+ ions can be ion exchange and chelation. PT-ED (26 mg) recovered gold and palladium rapidly from the co-existing metals in the printed circuit board (PCB) scrap, including Ca, Zn, Si, Cr, Pb, Ni, Cu, Ba, W, Co, Mn, and Mg with supreme selectivity toward gold and palladium. The results of this work suggest the use of PT-ED with high selectivity and efficiency to recover palladium and gold from secondary sources such as PCB scrap.

Green and low-temperature synthesis of the magnetic modified biochar under the air atmosphere for the adsorptive removal of heavy metal ions from wastewater: CCD-RSM experimental design with isotherm, kinetic, and thermodynamic studies.

The accumulation of heavy metal ions in living cells leads to biological damage, which makes the necessity of using new methods to effectively remove heavy metal ions from the environment more vital. In this work, a magnetic modified biochar was prepared under regular air atmosphere and low temperature (220 ºC) and used as a low-cost and green adsorbent for efficient adsorptive removal of cobalt (Co(II)) and Lead (Pb(II)) ions from contaminated waters. The adsorption process was modeled and optimized using CCD-RSM to maximize the removal efficiency of heavy metal ions, as well as was monitored in detail by isotherm, kinetic, and thermodynamic studies. The results show that the Langmuir maximum adsorption capacity of the adsorbent reached 237.92 mg g-1 (single) and 121.23 mg g-1 (binary) for Co(II) and 207.21 mg g-1 (single) and 106.56 mg g-1 (binary) for Pb(II) under the short time of 25 min and solution pH of 6.0. The kinetic studies revealed that the pseudo-first-order model was the best-fitted model to experimental data and indicated that the adsorption process was mostly through chemisorption. Also, thermodynamic studies showed that that adsorptive removal of Co(II)and Pb(II)ions followed an endothermic and spontaneous process. The reusability studies demonstrated that the adsorbent could be successfully regenerated with 5 mL of 0.1 mol L-1 HNO3 solution, and the adsorption efficiency was retaining about 90% after four adsorption-desorption cycles. Also, the results from using real water samples, including drinking water, groundwater, and river water, implied that the synthesized magnetic modified biochar was highly efficient for practical treatment processes. Overall, the results indicated that the proposed magnetic biochar can be considered as a cost-effective and efficient adsorbent for adsorptive removal of heavy metal ions from contaminated waters.

Evaluation of the Biodistribution of Arginine, glycine, aspartic acid peptide-modified Nanoliposomes Containing Curcumin in Rats.

Background: Liposomes, as a biological membrane, is successfully used for drug delivery, reduces toxicity in normal cells and improves bio-accessibility of the drug to the target cells. Curcumin, as a bioactive substance with pleiotropic biological activities, is an anti-inflammatory compound and has several anticancer effects in different cancers such as pancreatic and breast cancer. Objectives: This study was conducted to determine the bio-distribution of arginine-glycine-aspartic acid (RGD)-modified nanoliposomes containing curcumin in different tissues of rats. Materials and Methods: The amount of curcumin in each tissue was examined by HPLC analysis. The distribution of liposomal Hoechst in the rats was evaluated by using fluorescence spectrophotometry, live animal imaging analyses and histological methods. Results: HPLC analysis showed the mean of curcumin in the blood significantly increased in the liposomal curcumin modified with RGD compared to free curcumin. These results were confirmed by fluorescence measurement for RGD modified liposome containing Hoechst dye. There was negligible fluorescent intensity in the blood rats, which received Hoechst alone. Live animal imaging analysis showed the presence of fluorescent color in heart tissue for all groups. It was also detected in kidney tissue for liposomal Hoechst modified with RGD group. Conclusions: The present study demonstrated that RGD-modified nano-liposomes can significantly improve drug retention time in the blood of rats.

In-situ hydrothermal synthesis of CNT decorated by nano ZnS/CuO for simultaneous removal of acid food dyes from binary water samples.

The zinc sulfide/copper oxide-carbon nanotube nanocomposite (ZnS/CuO-CNT) was fabricated by using an in-situ hydrothermal synthesis method and was used for simultaneous ultrasound-assisted adsorptive removal of a binary mixture of ponceau 4R (P4R) and tartrazine (TA) acid food dyes from contaminated water. The as-synthesized ZnS/CuO-CNT was described by FESEM, XRD, FTIR, BET, and zeta potential analysis. The results included nested network morphology, high purity with the crystalline structure, oxygen-containing functional groups, mesoporous/micropores texture with cumulate interspace, specific surface area of 106.54 m2 g-1, and zero-point charge (pHzpc) of 5.3. In adsorption experiments, the simultaneous effect of main independent variables, including solution pH, adsorbent dosage, concentration of each dye, temperature, and sonication time on the removal efficiency of dyes was studied systematically using the central composite design (CCD) method based on response surface methodology (RSM). Also, the second-order multivariate equation was presented to determine the relationship between the removal efficiencies of P4R and AT dyes and six independent effective variables. The high correlation coefficient (R2 ≥ 0.99), significant p-value (P < 0.0001), and non-significant lack-of-fit (P > 0.05) showed the high accuracy, and validity of the proposed model to predict the removal efficiency of P4R and TA acid food dyes. The experimental removal efficiency for P4R and TA dyes was found to be 98.45 ± 2.54, and 99.21 ± 2.23, respectively. Also, the Langmuir maximum adsorption capacity for P4R and TA dyes was determined to be 190.1 mg g-1 and 183.5 mg g-1, respectively. Finally, the adsorbent's reusability was tested for six periods and could be reused repeatedly without significant reduction in adsorption performance.

Stachys pilifera Benth: A Review of Its Botany, Phytochemistry, Therapeutic Potential, and Toxicology.

Background: Stachys L. (Lamiaceae) includes more than 300 annual or perennial species growing in temperate regions of Southern Africa, the Mediterranean, America, and Asia. Stachys pilifera Benth (S. pilifera), also known as Marzeh Kuhi, is an endemic species from Iran. It is found in the mountainous habitats of the Zagros area. It has various traditional uses, and the phytochemical ingredients and some biological activities of this species have been examined in previous studies. Methods: PubMed, Science Direct, Google Scholar, Scopus, and Science Web databases were used to gather the data. The purpose of this review is to consolidate the scattered knowledge reported in the literature about botany, traditional uses, phytochemistry, pharmacological properties, and safety of S. pilifera and suggest its potential medicinal properties. Key Findings. In traditional Iranian medicine, S. pilifera manages various illnesses, such as rheumatoid arthritis, common cold, infections, asthma, and tussive. More than 30 compounds have been identified in S. pilifera essential oil. The compounds found in S. pilifera are phenolic compounds, monoterpenes, sesquiterpenes, flavonoids, alkaloids, and terpenoids, which have various properties such as antioxidant, nephroprotective, anti-inflammatory, antimicrobial, hepatoprotective, and anticancer properties. Conclusions: The literature reveals that S. pilifera is an essential source of bioactive phytochemicals and illustrates the unknown area of this plant for new investigations. Moreover, we recommend that future research focus on toxicology and quality control studies for S. pilifera to fill the knowledge gap and provide theoretical support for the plant's possible functional and clinical uses.

The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.

A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources. The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water. The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM). Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits. Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively. The adsorption isotherms and kinetics were better fitted with Langmuir and pseudo-second-order models, respectively. Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms. Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents. After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%. This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.

Experimental design for the optimization of paraquat removal from aqueous media using a fixed-bed column packed with Pinus Eldarica stalks activated carbon.

In this study, a fixed-bed column packed with an activated carbon (Pinus eldarica stalks (PES-AC)) was used to evaluate the performance of paraquat removal from wastewater. The effect of bed height, initial paraquat concentration, contact time, flow rate on the removal of paraquat was investigated using response surface methodology (RSM) based on central composite design (CCD). From the RSM model, the optimum experimental conditions to achieve 94.65% removal of paraquat were solution pH of 8.0, 6 mg L-1 of paraquat, 4 mL min-1 of flow rate, 0.8 cm of the bed height, and 40 min of contact time. The breakthrough data were significantly fitted with Thomas, bed depth services time (BDST), and Yoon-Nelson models. The high values of NBD (14.33, 32.29, and 54.46 mg L-1) and critical bed depth (0.396, 0.370, and 0.330 cm) obtained from BDST model revealed the high efficiency and suitability of the adsorbent. Adsorption of paraquat on PES-AC was strongly dependent on solution pH, indicating an electrostatic attraction mechanism.

A reusable mesoporous adsorbent for efficient treatment of hazardous triphenylmethane dye wastewater: RSM-CCD optimization and rapid microwave-assisted regeneration.

In this research, mesoporous calcium aluminate nanostructures (meso-CaAl2O4) were synthesized using a citric acid-assisted sol-gel auto-combustion process as the potential adsorbent to eliminate toxic triphenylmethane dye malachite green (MG) from synthetic/real effluent. The surface morphology of meso-CaAl2O4 was highly porous with nanometric size and non-homogeneous surface. The specific surface area, total pore volume, and BJH pore diameter of meso-CaAl2O4 were 148.5 m2 g-1, 1.39 cm3 g-1, and 19 nm, respectively. The meso-CaAl2O4 also showed a very high heat resistance, due to losing only 7.95% of its weight up to 800 °C, which is mainly related to the moisture loss. The optimal adsorption conditions were obtained based on response surface methods (RSM)-central composite design (CCD) techniques. The Langmuir isotherm model was used for fitting the adsorption measurements, which presented 587.5 mg g-1 as the maximum adsorption capacity of the dye. The data obtained from the adsorption kinetics model were found to correspond to the pseudo-second-order model. Also, the thermodynamic parameters including enthalpy change (ΔH°), entropy change (ΔS°), and Gibbs free energy change (ΔG°) indicated that MG dye adsorption by the meso-CaAl2O4 was feasible, endothermic, and occurred spontaneously. Furthermore, the meso-CaAl2O4 was regenerated by microwave irradiation under 900 W at 6 min, and the MG dye removal efficiency was remained over 90% after the five cycles of microwave regeneration.

Highly effective pre-concentration of thymol and carvacrol using nano-sized magnetic molecularly imprinted polymer based on experimental design optimization and their trace determination in summer savoury, Origanum majorana and Origanum vulgare extracts.

To ascertain thymol and carvacrol in pharmaceutical syrups, a valid and effective magnetic molecular imprinted polymer dispersive solid phase microextraction (MMIP-DSPME) process was developed in this study, which was in combination with a high performance liquid chromatography-ultra violet (HPLC-UV) technique for the assessment of thymol and carvacrol separation and pre-concentration. Contact time, eluent kind and volume, pH, the mass of the MMIP were all taken into consideration as key factors. Design expert and multi-objective response surface methodology (RSM) were used to optimize these variables. The mass of the MMIP, sample pH, eluent kind, time of sorption, the volume of eluent, and time of elution were 10 mg, 6, acetonitrile, 28 min, 200 µL, and 5.5 min, respectively, for the maximum extraction recovery of the analytes. The limit of detection (LOD) was 0.042 ng mL-1 at the optimal conditions, while the value for the limit of quantification (LOQ) was 0.140 ng mL-1. At the optimized conditions for thymol and carvacrol, the suggested MMIP sorbent had sorption capacities of 64.1 and 72.6 mg g-1, respectively. Furthermore, for triplicate measurements, the linear dynamic range (LDR) was 0.40-5000 ng mL-1, and the method's accuracy (RSD %) was 6.26%. The saturation magnetization for the MMIP was 19.0 emu g-1 obtained by VSM, allowing the sorbent to be separated quickly. The sorption experiments confirmed the large sorption capacity of the MMIP for thymol and carvacrol, as well as its homogeneous binding sites. The extraction recovery for thymol and carvacrol was 96.9-103.8% and 96.6-105.4%, respectively, at all spiked amounts (20, 100, 200, and 500 ng mL-1). The findings of seven desorption-regeneration cycles using MMIP demonstrated the high stability of the sorbent. The MMIP revealed a particular behavior of sorption for thymol and carvacrol, implying a selective, simple, effective, and flexible analytical method.

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects.

INTRODUCTION: Cholestasis is a liver disease caused by a malfunction of the hepato-biliary system. Oxidative stress as a systemic complication is the main characteristic of cholestasis. The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of Portulaca oleracea (PO) methanolic extract on liver dysfunction and tissue damage induced by bile duct ligation (BDL) in rats. MATERIALS AND METHODS: Twenty-eight male Wistar rats were randomly divided into four groups: sham control (SC), BDL alone, SC plus 500 mg/kg methanolic extract of PO orally for 1 week, and BDL plus 500 mg/kg methanolic extract of PO orally for 1 week. After 1 week, the animals were anesthetized, and the liver and blood samples were taken from each animal. Biochemical parameters, oxidative stress biomarkers, histopathological changes, as well as the gene expression of IL-1, TNF-α, TGF-ß, and α-SMA have been evaluated. RESULTS: The methanolic extract of PO at a dose of 500 mg/kg significantly decreased the plasma levels of aminotransferases, alkaline phosphatase as compared to BDL group (P < 0.05), while it had no significant effect on the levels of oxidative stress markers in the hepatic tissue. The plasma level of malondialdehyde and ferric-reducing antioxidant power were markedly elevated in the BDL group in comparison to SC group (P < 0.05), while treatment with PO significantly reduced these markers (P < 0.05). The administration of PO attenuated hydroxyproline content, bile duct proliferation, and inflammation score in the cholestatic liver in contrast to non-treated BDL rats (P < 0.05). Moreover, the methanolic extract of PO markedly declined the expression of TNF-α and TGF-ß pro inflammatory genes in contrast to BDL rats. CONCLUSIONS: Taken together, our findings showed that PO attenuated liver injury by decreasing liver function tests, inflammation, and hydroxyproline content. As a result, it is suggested that PO can be applied in cholestatic liver damage as a therapeutic or adjuvant agent.

Synthesis of magnetic tungsten disulfide/carbon nanotubes nanocomposite (WS2/Fe3O4/CNTs-NC) for highly efficient ultrasound-assisted rapid removal of amaranth and brilliant blue FCF hazardous dyes.

In this research, the potentiality of magnetic tungsten disulfide/carbon nanotubes nanocomposite (WS2/Fe3O4/CNTs-NC) as an adsorbent for the ultrasound-assisted removal of amaranth (AM) and brilliant blue FCF (BB FCF) dyes was investigated. The experiments were conducted using a central composite design (CCD) with the inputs of solution pH (X1: 2.0-10), adsorbent mass (X4: 4-20 mg), AM concentration (X2: 10-50 mg L-1), BB FCF concentration (X3: 10-50 mg L-1), and sonication time (X5: 2-12 min). At the optimum conditions, the removal percentages of 99.30% and 98.50% were obtained for AM and BB FCF, respectively. The adsorption of the dyes was described by Langmuir isotherm and pseudo-second-order (PSO) kinetic models. The maximum adsorption capacities of AM and BB FCF were 174.8 mg g-1 and 166.7 mg g-1, respectively. The adsorption thermodynamic study showed that the adsorption of the dyes occurred endothermically and spontaneously. The removal percentages of AM and BB FCF from the real samples were in the range of 94.52-99.65% for the binary solutions. The removal percentage for each dye after five cycles of adsorption/desorption was > 90%. This work provides a useful insight to the potential application of CNTs-based magnetic nanocomposite for the treatment of wastewaters contaminated with dyes.

Protective Effects of Hydroalcoholic Extract of Rosa canina Fruit on Vancomycin-Induced Nephrotoxicity in Rats.

Vancomycin-induced nephrotoxicity (VIN) has been reported to occur in 5-35% of recipient patients. The aims of the study were to evaluate protective effects of Rosa canina (RC) on VIN in rats. Rats were randomly divided into five groups as follows: control group I, group II (received VAN 400 mg/kg/day, every 12 h at doses of 200 mg/kg/day, for 7 consecutive days), group III (VAN + RC 250 mg/kg/day, for 7 consecutive days), group IV (VAN + RC 500 mg/kg/day, for consecutive days), and group V (received RC 500 mg/kg/day, for consecutive 7 days). On the eighth day after anesthetizing the animals, blood samples were taken from the heart, and then, the kidneys were removed to investigate kidney function, oxidative stress, and histopathological marker. Also, the chemical composition of RC extract was identified by GC-MS analysis. Oral dose of 500 mg/kg RC extract significantly reduced the serum levels of blood urea nitrogen (BUN), creatinine (Cr), malondialdehyde (MDA), and nitric oxide (NO) and also the kidney tissue MDA, protein carbonyl, and NO metabolites (nitrite) levels compared to the VAN-treated group (P < 0.05). Based on histopathological analysis, RC extract at the dose of 500 mg/kg inhibited the destructive effects of VAN on kidney tissues. GC-MS analysis indicated that the main compositions were found to be lactose (21.96%), 3-t-butyloxaziridine (20.91%), and 5-oxymethylfurfurole (16.75%). The results indicated that oral administration of RC was able to reduce VAN-induced nephrotoxicity in rats, possibly through antioxidant pathways.

The Effect of the Hydroalcoholic Extract of Watercress on the Levels of Protein Carbonyl, Inflammatory Markers, and Vitamin E in Chronic Hemodialysis Patients.

INTRODUCTION: Chronic kidney disorder is a main public health concern. Inflammatory processes and oxidative stress are common in end-stage renal disease patients. We aimed to evaluate the effect of the hydroalcoholic extract of watercress (WC) on the inflammatory cytokines and protein carbonyl (PCO) contents in chronic hemodialysis patients. METHODS: This was a double-blind randomized clinical trial performed on 46 hemodialysis patients. The participants were randomly divided into two groups: intervention group (500 mg hydroalcoholic extract of WC every day for 4 weeks) and control group (500 mg of white flour every night for 4 weeks). The blood samples were taken to determine the levels of vitamin E, PCO, and inflammatory cytokines at baseline and the end of treatment. RESULTS: Forty-five patients completed the study (22 patients in the intervention group and 23 patients in the control group). There was a significant reduction in the PCO level (20.33 ± 4.40 vs. 15.06 ± 6.41, P=0.001) in the intervention group; also, this change was statistically significant relative to the control group. Furthermore, there were significant reductions in hs-CRP (8953.30 ± 5588.06 vs. 7249.86 ± 5091.62, P=0.007) and IL-6 (60.10 (55.99, 73.10) vs. 55.21 (53.39, 60.48), P=0.050) in the intervention group, but these changes were not significant in comparison with the control group. CONCLUSION: We conclude that the hydroalcoholic extract of WC reduced the PCO content in hemodialysis patients via inhibition of protein oxidation. Although WC administration had caused a significant reduction in IL-6 and CRP levels, these differences were not statistically significant relative to the control group. Further research is needed to identify the antioxidant and anti-inflammatory effects of WC in hemodialysis patients.

Ultrasound-assisted solid phase microextraction-HPLC method based on Fe3O4@SiO2-NH2-molecularly imprinted polymer magnetic nano-sorbent for rapid and efficient extraction of harmaline from Peganum harmala extract.

In the present study, a magnetic molecularly imprinted polymer (MMIP) was synthesized for the extraction of harmaline from Peganum harmala by dispersive solid-phase microextraction (DSPME). The MMIP for selective and intelligent extraction of harmaline with excellent functionality and high selectivity was synthesized using the sol-gel method with functionalized superparamagnetic core-shell nanoparticles, ethylene glycol dimethacrylate (EDMA) as a cross-linker, methacrylic acid (MAA) as a functional monomer, and 2,2-azobisisobutyronitrile (AIBN) as a porogen. To study the properties and morphology of the coated polymer, FT-IR spectroscopy, FESEM, TEM images, and VSM were used. The DSPME-HPLC-UV equipment was used to quantify and analyze the data obtained from harmaline extraction. In this research, the efficiency of the synthesized polymer in harmaline extraction was modeled and optimized using the response surface methodology based on central composite design (RSM-CCD). In addition, for modeling the isotherm of harmaline sorption by the MMIP, Langmuir and Freundlich isotherm equations were used. The obtained results showed that the extraction of harmaline with the MMIP was well described with Freundlich isotherm. The results of the validation of the method showed that the measurement of harmaline in the concentration range of 1.0-4000 ng mL-1 followed a linear relationship (R2 = 9986.0). Moreover, the accuracy or repeatability index (% RSD) was determined to be < 10, and the LOQ and LOD values were 0.526 and 0.158 ng mL-1, respectively. The results of this study showed that the DSPME technique by using the synthesized MMIP as an effective sorbent with high efficiency and capacity could be utilized for pre-concentration and extraction of harmaline from real and complex samples.

Effects of Nasturtium officinale Extract on Antioxidant and Biochemical Parameters in Hemodialysis Patients: A Randomized Double-Blind Clinical Trial.

BACKGROUND: Increased oxidative stress play an important role in the risk of cardiovascular disease, mortality, and mortality patients undergoing dialysis. Nasturtium officinale (watercress) contains numerous phytochemical compounds that act as an antioxidant by preventing oxidative damage to biomolecules. Therefore, this research aimed to explore the effect of the ethanolic extract of Nasturtium officinale (EENO) on antioxidant and biochemical markers of hemodialysis patients. METHODS: In this double-blind, placebo-controlled trial, 46 hemodialysis patients were randomly recruited to consume either 500 mg/day EENO (n = 23) or placebo capsule (n = 23) for 4 weeks, at Shahid Beheshti Hospital, Yasuj, Iran, in 2019. Biomarkers of oxidative stress including glutathione peroxidase (GPX), superoxide dismutase (SOD), malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), and total sulfhydryl protein (T-SH) and biochemical parameters such as BUN, Hb, WBC, PLT, Ca, Ph, K, ALB, TChol, TG, LDL, and HDL were evaluated on days 0 and 28. RESULTS: The serum levels of MDA and BUN significantly decreased after taking EENO supplementation (P < 0.001); however, SOD activity increased during the same period (P < 0.001). The serum levels of TAC remained constant in the intervention group, while it significantly declined in the placebo group (P < 0.09). The extract also prevented elevation in the serum levels of LDL and TG compared to the placebo group, although it was not statistically significant. CONCLUSIONS: The data indicated that the consumption of EENO improved some of the antioxidant parameters and minimizes the change in TG and LDL in hemodialysis patients. Therefore, due to the role of these factors in mortality and morbidity of dialysis patients, EENO can improve the condition of dialysis patients. However, more studies with longer intervention times and different doses of EENO are recommended.

Decorating graphene oxide with zeolitic imidazolate framework (ZIF-8) and pseudo-boehmite offers ultra-high adsorption capacity of diclofenac in hospital effluents.

This study reports on an easy and scalable synthesis method of a novel magnetic nanocomposite (GO/ZIF-8/γ-AlOOH) based on graphene oxide (GO) nanosheets decorated with zeolitic imidazolate framework-8 (ZIF-8), pseudo-boehmite (γ-AlOOH), and iron oxide (Fe3O4) nanoparticles by combining solvothermal and solid-state dispersion (SSD) methods. The nanocomposite was successfully applied to remove of diclofenac sodium (DCF) - a widely used pharmaceutical - from water. Response Surface Methodology (RSM) was used to optimize the adsorption process and assess the interactions among the influencing factors on DCF removal efficiency; including contact time, adsorbent dosage, initial pH, solution temperature, and DCF concentration. Adsorption isotherm results showed a good fitting with the Langmuir isotherm model with an exceptional adsorption capacity value of 2594 mg g-1 at 30 °C, which was highly superior to the previously reported adsorbents. In addition, kinetic and thermodynamic investigations further illustrated that the adsorption process was fast (equilibrium time = 50 min) and endothermic. The regeneration of GO/ZIF-8/γ-AlOOH nanocomposite using acetic acid solution (10% v/v) after a simple magnetic separation was confirmed in five consecutive cycles, which eliminate the usage of organic solvents. The nanocomposite has also shown a superior performance in treating a simulated hospital effluent that contained various pharmaceuticals as well as other organic, and inorganic constituents.

Simultaneous selective enrichment of methylparaben, propylparaben, and butylparaben from cosmetics samples based on syringe-to-syringe magnetic fluid phase microextraction.

Present work is the preparation of novel magnetic nanofluids based on deep eutectic solvent and used for the rapid microextraction of methylparaben (MP), propylparaben (PP), and butylparaben (BP) from cosmetics samples using syringe-to-syringe dispersive magnetic nanofluid microextraction procedure (SS-DMNF-ME). The optimization of the extraction of MP, PP, and BP was performed through central composite design (CCD). The optimum extraction conditions were assessed by optimizing pH, nanofluid volume, NaCl concentration, cycle number, and methanol volume. pH 8.0, 200 µL of magnetic nanofluid, 6% w/v of NaCl, eight cycles of injection/back injection, and 80 µL of methanol were the optimum extraction conditions, with the maximum recoveries of 98.62%, 100.92%, and 99.13% for MP, PP, and BP, respectively. The figures of merit calculated under the optimum condition were achieved from the CCD, and the developed method exhibited the low limits of quantitation (4.3, 3.0, and 2.7 ng mL-1) and detection (1.3, 0.9, and 0.8 ng mL-1) for MP, PP, and BP, respectively, as well as excellent linearity with R2 > 0.99. The relative recoveries of three parabens in the actual samples were 85.99-99.07% with relative standard deviations ≤5.52%. In comparison to other extraction methods, SS-DMNF-ME was readily and rapidly determined MP, PP, and BP using HPLC-UV, and experimental data showed the efficiency, robustness, and reliability of the proposed method.

Nano-sized Fe3O4@SiO2-molecular imprinted polymer as a sorbent for dispersive solid-phase microextraction of melatonin in the methanolic extract of Portulaca oleracea, biological, and water samples.

In this study, a magnetic molecularly imprinted polymer (MMIP (Fe3O4@SiO2-MIP)) was used for the dispersive magnetic solid-phase microextraction (d-MSP-µ-E) to design an easy and effective method for melatonin (MLT) extraction in the methanolic extract of Portulaca oleracea, human urine and plasma, and water samples. HPLC with UV detection was utilized, and pH, the type and volume of eluent, MMIP mass, and contact time were considered as effective factors in the study of MLT separation and pre-concentration. These factors were optimized by Plackett-Burman and multi-objective response surface methodology (RSM). The values were 10 mg, 14 min, 4.2, methanol, 0.180 mL, 2.5 min, for the MMIP mass, time of sorption, sample pH, eluent type, eluent volume, and time of elution, respectively. At the optimum conditions, the limit of detection (LOD) was 0.046 ng mL-1, and the limit of quantification (LOQ) was 0.156 ng mL-1. The sorption capacity of the proposed MMIP sorbent was 109.1 mg g-1 at the optimum conditions. Besides, linear dynamic range (LDR) was 0.2-4200 ng mL-1, and the precision of the method (RSD %) for triplicate measurements was <6.1%. The MMIP showed saturation magnetization of 19.75 emu g-1, resulting in fast separation of the sorbent. The sorption test revealed the high sorption capacity of the MMIP for MLT and its homogeneous binding sites. In all spiked levels (50, 100, 200, and 500 ng mL-1), 93.07-104.1% was the range obtained for the recovery of MLT. The relative selectivity factor (ß) values of MLT/tryptophan, MLT/serotonin, MLT/ferulic acid, MLT/mefenamic acid, MLT/quercetin, MLT/luteolin, and MLT/chlorogenic acid were 1.60, 1.68, 2.02, 2.38, 2.32, 2.40, and 2.50, respectively. The results of desorption-regeneration cycles (seven times) by employing the MMIP showed the high stability of the resultant material. In conclusion, the MMIP combined with the magnetic separation showed a specific sorption behavior for MLT and suggested a simple, flexible, selective, and powerful analytical tool.

A new approach for microextraction of trace albendazole sulfoxide drug from the samples of human plasma and urine, and water by the molecularly imprinted polymer nanoparticles combined with HPLC.

In this research study, a method of dispersive-micro-solid phase extraction (D-µ-SPE) combined with molecularly imprinted polymer nanoparticles (MIP-NPs) with HPLC-UV was developed for the fast and selective detection of the trace amount of albendazole sulfoxide (ABZSO) in the biological samples. To investigate the effective factors on ABZSO microextraction by the method, central composite design (CCD) was utilized, and the optimum conditions for ABZSO microextraction were sample pH of 8.0, MIP-mass of 15 mg, sonication time of 12 min, and eluent (methanol) volume of 0.25 mL. Under the obtained optimal extraction conditions, the value for the limit of detection (LOD) and limit of quantification (LOQ) was respectively showed to be 0.074 and 0.246 ng mL-1. In addition, the calculated peak areas exhibited a linear relationship with the ABZSO concentration ranging from 0.4 to 4200 ng mL-1. The analyses of the samples including human plasma and urine, and water were successfully performed by the usage of the D-µ-SPE method, which was a simple and sensitive technique and a suitable alternative for the analysis of ABZSO. In the analysis of ABZSO in various samples, the recoveries at various levels of ABZSO concentrations (50, 300, and 500 ng mL-1) were in the range of 95.7-103.0 %, and the relative standard deviations (RSDs; n = 3) varied from 2.2 to 4.4%.