DNA damage in workers exposed to mineral oils.

Mineral oils, untreated or mildly treated, have been classified in group 1 as a potential source of cancer by the International Agency for Research on Cancer (IARC). Although numerous studies have implicated metalworking fluids (MWFs) as human carcinogens, toxicology data regarding the mechanism of carcinogenicity are limited. This study is intended to examine the systemic effects of machining workers' exposure to MWFs. The potential toxicity of mineral oils was investigated in 65 lathe workers compared to controls (66 men). The occupational exposure was measured by the National Institute for Occupational Safety and Health (NIOSH) 5026. The DNA damage has been examined by the comet assay method. According to the field assessments, the time-weighted average (TWA) exposure to mineral oil mist was 7.67 ± 3.21 mg/m3. A comet assay of peripheral blood cells showed that tail length (TL) and olive moment (OM) were significantly higher in the exposed group (p < 0.05). A multiple logistic regression analysis revealed that, within subjects with over 10 years of exposure, the odds ratio of worker with high TL, percent of DNA in tail, OM, and tail moment (TM) were 1.68, 1.41, 1.71, and 2.71, respectively. DNA strand break in exposed workers was associated with higher exposure time in years. Mineral oil toxicity could be altered in the presence of by-products and impurities. For a better understanding of genotoxicity, further studies are required.

Risk assessment of chemical mixtures by benchmark dose-principle component analysis approach in occupational exposure.

Mixtures of organic solvents are widely used in industrial processes. Risk assessment for chemical co-exposure has always been a challenge in past years. The present study aims to employ principle component analysis (PCA) to produce an entry for benchmark dose approximation in shoemakers based on the color vision effect. A total of 134 subjects consisting of 67 shoemakers and 67 staff workers were employed for Benchmark Dose (BMD) evaluation. Occupational exposure to benzene, toluene, xylene, and n-hexane was evaluated using NIOSH 1501 and OSHA ID-07 methods. The color vision effect was quantified using Lanthony D-15 desaturated test (D-15d). PCA was run for cumulative exposure dose (CED) of the solvents by MATLAB 2018. Finally, the lowest 95% confidence limit of the benchmark dose (BMDL) was determined using US EPA benchmark dose software (BMDS) version 3.2.1. The color confusion index (CCI) level in shoemakers increased from 1 to 1.15 by a median of 1.07. There was a significant difference in the CCI level (p value<0.0001) between exposed and control subjects. The first score of PCA was used as intake dose level (IDL) in solvents co-exposure. Using BMD analysis, the log-logistics model was fitted with a p-value> 0.1 and the lowest BMDL level. BMDL level was evaluated at 1.63, 10.25, 2.21, and 3.35 ppm for benzene, toluene, xylene, and n-hexane, respectively. The results showed a risk of color vision effect with co-exposure to solvents at different levels in the occupational exposure standards. In conclusion BMDL-PCA approach has been suggested for the risk assessment of chemical co-exposure.