Radiological and histological evaluation of horizontal ridge augmentation using corticocancellous freeze-dried bone allograft with and without autogenous bone: A randomized controlled clinical trial.

PURPOSE: The purpose of this study was radiological and histological evaluation of horizontal ridge augmentation using corticocancellous freeze-dried bone allograft (FDBA) with and without autogenous bone (AB). MATERIALS AND METHODS: The present research was conducted on 42 patients (27 females and 15 males) with insufficient width of edentulous ridge. The patients were randomly assigned into two groups, FDBA alone + collagen membrane (n = 21) and the combined FDBA and AB + collagen membrane (n = 21). The horizontal alveolar ridge dimensions were measured using cone-beam computerized tomography before and 6 months after alveolar ridge augmentation. At the time of insertion of implants, biopsy of new bone was taken from 11 patients in each group and was analyzed histologically. The obtained data were statistically analyzed with paired t test and two-sample t test. The registration number was IRCT201109165305N3. RESULTS: The mean ± SD ridge width gain after 6 months at the distance of 0, 2, 4, and 6 mm from crest of alveolar ridge was 2.78 ± 1.44, 3.05 ± 1.21, 2.82 ± 1.62, and 2.23 ± 1.95 mm in the FDBA group and 2.40 ± 1.60, 3.10 ± 1.80, 3.60 ± 1.87, and 2.65 ± 2.39 mm in the combined group, respectively, which was statistically significant in both groups using paired t test (P < .001). However, the difference between two groups analyzed by two-sample t test was not statistically significant (P > .05). Amount of new bone generation, remained particles, and connective tissue was not statistically different between two groups (P = .367, P = .428, and P = .598, respectively). CONCLUSION: Based on the results of this study, corticocancellous FDBA granules along with collagen membrane can successfully be used for horizontal augmentation of edentulous ridge, and adding AB to the granules of FDBA does not significantly increase the quality and quantity of regenerated bone.

Immunohistochemical Evaluation of P53 and Epidermal Growth Factor Receptor Proteins Expression Levels in Gingival Tissue of Opium-dependent Patients.

BACKGROUND: Although evidence indicates that tobacco use is one of the risk factors for oral cancer, the relationship between opium addiction and oral cancer has not yet been evaluated. The present study was performed aiming to evaluate P53 and epidermal growth factor receptor (EGFR) expression in the gingival tissue of opium-dependent patients. METHODS: 102 individuals (70 men and 32 women) were entered in the study. 63 and 39 individuals were included in the opium-dependent group and opium-independent group (control group), respectively. 1 gingival biopsy was taken from each individual. The biopsies were fixed in 10% buffered formaldehyde solution and embedded in paraffin at 56 °C. The slides were then stained with Hematoxylin-Eosin (H & E) and Immunohistochemistry (IHC) evaluation was performed with the antihuman antibodies of P53 and EGFR. The protein expression level was later assessed and data were analyzed statistically. FINDINGS: P53 expression was higher among the opium-dependent group, however the difference was not statistically significant (P = 0.052). EGFR expression was significantly higher among the opium-dependent group compared to the control group (P = 0.006). CONCLUSION: Opium dependency significantly affects EGFR expression in gingival tissue, however it seems to have no significant effect on P53 expression.