RESUMO
Considering mitigating oxidative degradation and inhibiting microbial proliferation in meat products, incorporating antioxidant and antimicrobial materials is critical to enhance shelf life, maintain quality, and ensure food safety. So, this study aimed to investigate the antimicrobial and antioxidant effects of encapsulated gallic acid on the quality of hamburgers during 30 days of storage. Gallic acid was microencapsulated in tragacanth gum/gelatin complex coacervate, and its encapsulation efficiency was optimized by the response surface method. The optimized encapsulation conditions were 1:4 polymer ratio (tragacanth to gelatin ratio); total polymer content, 0.9 %; pH, 3.5; and gallic acid content, 0.88 %, resulting in a 98 % encapsulation efficiency. The microcapsules were characterized using various techniques, including scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry. 400 ppm encapsulated gallic acid was added to the hamburger formulation, and various microbial properties, chemical analysis (peroxide value (POV) and thiobarbituric acid (TBA)), and sensory properties of the hamburgers were evaluated during storage. Results showed that gallic acid in the hamburgers decreased lipid oxidation from 0.126 to 0.103 mg MAD/kg in the TBA test and 12.73 to 11.03 meq/kg in the POV test during one month of storage. Also, phenolic compounds could prevent the growth and proliferation of spoilage microorganisms by damaging the microorganism cell walls and changing the metabolic processes. So, the amounts of total count and yeast and mold in the treated sample were lower than in the control sample. Significantly, adding encapsulated gallic acid did not negatively affect the flavor or overall evaluation of the samples. Overall, these findings suggested that encapsulated gallic acid is a suitable candidate to maintain chemical, microbial, and sensory characteristics of hamburgers over time.
RESUMO
In this study iron salt (FeSO4·7H2O) was microencapsulated in gum tragacanth hydrogel using solvent evaporation method. Three significant parameters (ferrous sulfate content, content of gum tragacanth, and alcohol to mixture ratio) were optimized by response surface methodology to obtain maximum encapsulation efficiency. Ferrous sulfate content, 5%, content of gum tragacanth, 22%, and alcohol to mixture ratio, 11:1 was determined to be the optimum condition to reach maximum encapsulation efficiency. Microstructure of iron microcapsules was thoroughly monitored using scanning electron microscopy (SEM). The microphotographs indicated two distinct crystalline and amorphous structures in the microcapsules. This structure was confirmed by X-ray diffraction (XRD) pattern of microcapsules. Fourier transform infrared (FTIR) spectra of iron microcapsules identified the presence of iron in the tragacanth microcapsules. The average size of microcapsules was determined by particle size analyzer. Release assessment of iron in simulated gastric fluid showed its complete release in stomach which is necessary for its absorption in duodenum. However, the use of encapsulated iron in gum tragacanth in watery foods is rather recommended due to the fast release of iron in water.
