Publisher Correction: Tidal and diel orchestration of behaviour and gene expression in an intertidal mollusc.

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Tidal and diel orchestration of behaviour and gene expression in an intertidal mollusc.

Intertidal inhabitants are exposed to the 24-hour solar day, and the 12.4 hour rising and falling of the tides. One or both of these cycles govern intertidal organisms' behaviour and physiology, yet little is known about the molecular clockworks of tidal rhythmicity. Here, we show that the limpet Cellana rota exhibits robust tidally rhythmic behaviour and gene expression. We assembled a de-novo transcriptome, identifying novel tidal, along with known circadian clock genes. Surprisingly, most of the putative circadian clock genes, lack a typical rhythmicity. We identified numerous tidally rhythmic genes and pathways commonly associated with the circadian clock. We show that not only is the behaviour of an intertidal organism in tune with the tides, but so too are many of its genes and pathways. These findings highlight the plasticity of biological timekeeping in nature, strengthening the growing notion that the role of 'canonical' circadian clock genes may be more fluid than previously thought, as exhibited in an organism which has evolved in an environment where tidal oscillations are the dominant driving force.

Changing relative crank angle increases the metabolic cost of leg cycling.

PURPOSE: Historically, the efficiency of leg cycling has been difficult to change. However, arm cycling research indicates that relative crank angle changes can improve efficiency. Therefore, we investigated if leg cycling with different relative crank angles affects efficiency. METHODS: Ten healthy, male, recreational bicycle riders (27.8 ± 8.2 years, mean ± SD, mass 69.8 ± 3.2 kg) pedaled a pan-loaded cycle ergometer at a fixed power output of 150 watts at a cadence of 90 RPM. Each subject completed six, 5-min trials in random order at relative crank angles of 180°, 135°, 90°, 45°, 0°, and 180°. We averaged rates of oxygen uptake ([Formula: see text]) and carbon dioxide production ([Formula: see text]), and respiratory exchange ratio (RER) for the last 2 min of each trial. RESULTS: Crank angles other than 180° required a greater metabolic cost. As relative crank angle decreased from 180°, metabolic power monotonically increased by 1.6% at 135° to 8.2% greater when the relative crank angle was 0° (p < 0.001). CONCLUSIONS: We find that, unlike arm cycling, radically changing the relative crank angle on a bicycle from an out-of-phase (180°) to in-phase (0°) position decreases leg cycling efficiency by ~8%. We attribute the increase to changes in cost of breathing, muscle co-activation, trunk stabilization, power fluctuations, and possibly lifting the legs during the upstroke. Our findings may have relevance in the rehabilitation of patients recovering from stroke or spinal cord injury.