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2.
Neuroscience ; 428: 112-121, 2020 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-31917348

RESUMO

To understand neuronal information processing, it is essential to investigate the input-output relationship and its modulation via detailed dissections of synaptic transmission between pre- and postsynaptic neurons. In Caenorhabditis elegans, pre-exposure to an odorant for five minutes reduces chemotaxis (early adaptation). AWC sensory neurons and AIY interneurons are crucial for this adaptation; AWC neurons sense volatile odors, and AIY interneurons receive glutamatergic inputs from AWC neurons. However, modulations via early adaptation of the input-output relationship between AWC and AIY are not well characterized. Here we use a variety of fluorescent imaging techniques to show that reduced synaptic-vesicle release without Ca2+ modulation in AWC neurons suppresses the Ca2+ response in AIY neurons via early adaptation. First, early adaptation modulates the Ca2+ response in AIY but not AWC neurons. Adaptation in the Ca2+ signal measured in AIY neurons is caused by adaptation in glutamate release from AWC neurons. Further, we found that a G protein γ-subunit, GPC-1, is related to modulation of glutamate input to AIY. Our results dissect the modulation of the pre- and postsynaptic relationship in vivo based on optical methods, and demonstrate the importance of neurotransmitter-release modulation in presynaptic neurons without Ca2+ modulation.


Assuntos
Proteínas de Caenorhabditis elegans/metabolismo , Plasticidade Neuronal/fisiologia , Células Receptoras Sensoriais/fisiologia , Olfato/fisiologia , Adaptação Fisiológica/fisiologia , Animais , Caenorhabditis elegans , Ácido Glutâmico/metabolismo , Transmissão Sináptica/fisiologia
3.
J Am Chem Soc ; 142(3): 1594-1602, 2020 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-31868355

RESUMO

γ-Lactam derivatives with multiple contiguous stereogenic carbon centers are ubiquitous in physiologically active compounds. The development of straightforward and reliable synthetic routes to such chiral structural motifs in a stereocontrolled manner should thus be of importance. Herein, we report a strategy to construct polycyclic γ-lactam derivatives that contain more than two contiguous stereogenic centers in an enantioselective as well as atom-economic manner. Moreover, we have achieved the first enantioselective synthesis of strigolactam derivative GR-24, a racemic variant of which is a potential seed germination stimulator and plant-growth regulator. A key of the procedure presented here is a nickel(0)/chiral phosphoramidite-catalyzed asymmetric [2+2+1] carbonylative cycloaddition between readily accessible ene-imines and carbon monoxide, which proceeded enantioselectively to furnish up to 90% ee (>99% ee after recrystallization). The results of mechanistic studies, including the isolation of a chiral heteronickelacycle, support that the enantioselectivity on the two contiguous carbon atoms of the γ-lactams is determined during the oxidative cyclization on nickel(0).

4.
PLoS One ; 14(12): e0226044, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800640

RESUMO

Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5-HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous research has shown that the neural activity of the RID interneuron increases when the worm moves forward, and the RID is necessary for sustaining forward locomotion. However, the relationship between 5-HT and neural activity of RID, and how it modulates the behavior of the worm has not been investigated. In this article, we reveal the relationship among 5-HT, RID activity, and the behavior of worms using a custom-made tracking and imaging system. We simultaneously measured the neural activity of the RID and behavior in worms with three conditions: mock animals, animals pre-exposed to 5-HT, and 5-HT receptor mod-1 mutants. As shown in previous research, the neural activity of the RID increased during the transition from backward to forward, whereas it decreased during the transition from forward to backward in mock animals. These changes in neural activity were not observed in animals pre-exposed to 5-HT and mod-1 mutants. Moreover, RID activity was correlated with the velocity of the worm in mock animals. However, this correlation was not observed in animals pre-exposed to 5-HT and mod-1 mutants. Our results demonstrate that 5-HT modulates the activity of the RID interneuron, and we infer that the RID plays a role in modulating forward locomotion by changing its activity through 5-HT.


Assuntos
Interneurônios/efeitos dos fármacos , Serotonina/farmacologia , Animais , Caenorhabditis elegans/fisiologia , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Cálcio/metabolismo , Canais de Cloreto/genética , Canais de Cloreto/metabolismo , Interneurônios/fisiologia , Locomoção/efeitos dos fármacos , Mutagênese
5.
iScience ; 19: 191-203, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31377664

RESUMO

Determining how neurotransmitter input causes various neuronal activities is crucial to understanding neuronal information processing. In Caenorhabditis elegans, AIY interneurons receive several sources of sensory information as glutamate inputs and regulate behavior by integrating these inputs. However, the relationship between glutamate input and the Ca2+ response in AIY under environmental noise, in other words, without explicit stimulation, remains unknown. Here, we show that glutamate-input fluctuations evoke a sporadic Ca2+ response in AIY without stimulation. To ensure that Ca2+ response can be considered AIY output, we show that the membrane-potential depolarization precedes Ca2+ responses in AIY. We used an odor as model stimulation to modulate the sensory inputs. Simultaneous imaging of glutamate input and Ca2+ response, together with glutamate transmission mutants, showed that glutamate-input fluctuations evoke sporadic Ca2+ responses. We identified the input-output relationships under environmental noise in vivo, and our results address the relationship between sensory-input fluctuations and behavioral variability.

6.
Neurosci Lett ; 706: 68-74, 2019 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-31082452

RESUMO

Food exploration is an essential survival behavior in organisms. To find food efficiently, many organisms use a foraging strategy called area-restricted search (ARS) wherein individuals first turn more frequently, restricting their search to one area, then turn less frequently, moving along a straight path to widen the search area. Previous research suggests that the nematode Caenorhabditis elegans shows ARS behavior by changing turn frequency, and that dopamine is a crucial determinant. However, the effects of dopamine on multiple behavioral parameters have remained unknown. Here, we evaluated turn (pirouette) frequency, moving velocity, and specific area occupancy (cell occupancy) over time by using a multiple-worms tracking system. In the control (mock) experiments, all parameters changed over time, but no changes were observed in experiments with dopamine pre-exposed and dopamine-deficient animals. In inverse reinforcement learning analysis, the value function for specific velocity was found to modulate over time in mock animals only. These results demonstrate that dopamine regulates ARS via changes not only to pirouette frequency change but also to velocity.


Assuntos
Comportamento Apetitivo/efeitos dos fármacos , Caenorhabditis elegans/efeitos dos fármacos , Dopamina/farmacologia , Aprendizado de Máquina , Animais , Animais Geneticamente Modificados , Reforço Psicológico
7.
Biophys Physicobiol ; 16: 167-172, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31984168

RESUMO

Exploring for food is important in food-deprived condition. Chemotaxis is one of the important behaviors to search food. Although chemotactic strategies in C. elegans have been well investigated: the pirouette and the weathervane strategies, the change of the chemotactic strategy by food deprivation is largely unclear. Here, we show the change of chemotactic strategy by food deprivation, especially for isoamyl alcohol. To compare chemotaxis under different food-deprivation period, we showed that worms change their chemotactic behaviors by food deprivation. The worms with 1-h food-deprivation change the weathervane strategy. On the other hand, 6-h food deprived animals change the pirouette strategy. These results demonstrate that worms change chemotactic strategy different way depend on period of food deprivation.

8.
Biochem Biophys Res Commun ; 508(3): 690-694, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30528391

RESUMO

Biological systems process information under noisy environment. Sensory adaptation model of E. coli is suitable for investigation because of its simplicity. To understand the adaptation processing quantitatively, stochastic thermodynamic approach has been attempted. Information processing can be assumed as state transition of a system that consists of signal transduction molecules using thermodynamic approach, and efficiency can be measured as thermodynamic cost. Recently, using information geometry and stochastic thermodynamics, a relationship between speed of the transition and the thermodynamic cost has been investigated for a chemical reaction model. Here, we introduce this approach to sensory adaptation model of E. coli, and examined a relationship between adaptation speed and the thermodynamic cost, and efficiency of the adaptation speed. For increasing external noise level in stimulation, the efficiency decreased, but the efficiency was highly robust to external stimulation strength. Moreover, we demonstrated that there is the best noise to achieve the adaptation in the aspect of thermodynamic efficiency. Our quantification method provides a framework to understand the adaptation speed and the thermodynamic cost for various biological systems.


Assuntos
Adaptação Fisiológica , Escherichia coli/fisiologia , Simulação por Computador , Teoria da Informação , Modelos Biológicos , Análise Numérica Assistida por Computador , Processos Estocásticos , Termodinâmica
9.
Angew Chem Int Ed Engl ; 57(35): 11418-11421, 2018 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-29998548

RESUMO

The carbon-carbon (C-C) bond activation of [n]cycloparaphenylenes ([n]CPPs) by a transition-metal complex is herein reported. The Pt0 complex Pt(PPh3 )4 regioselectively cleaves two C-C σ bonds of [5] CPP and [6]CPP to give cyclic dinuclear platinum complexes in high yields. Theoretical calculations reveal that the relief of ring strain drives the reaction. The cyclic complex was further transformed into a cyclic diketone by using a CO insertion reaction.

10.
J Exp Biol ; 221(Pt 11)2018 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-29691312

RESUMO

In many species, individual social animals interact with others in their group and change their collective behaviours. For the solitary nematode Caenorhabditis elegans strain N2, previous research suggests that individuals can change the behaviour of other worms via pheromones and mechanosensory interactions. In particular, pheromones affect foraging behaviour, so that the chemotactic behaviours of individuals in a group (population) can be modulated by interactions with other individuals in the population. To investigate this, we directly compared the chemotactic behaviours of isolated (single) worms with those of individual animals within a population. We found that worms approached an odour source in a distinct manner depending on whether they were alone or in a population. Analysis of behaviours of the N2 worm and a pheromone production-defective mutant revealed that the 'pirouette' strategy was modulated by interaction of the worms via pheromones. Thus, pheromones play an important role in the characteristic collective behaviours seen in the population condition.


Assuntos
Caenorhabditis elegans/fisiologia , Quimiotaxia/fisiologia , Feromônios/metabolismo , Animais , Comportamento Alimentar , Densidade Demográfica
11.
Angew Chem Int Ed Engl ; 56(28): 8206-8210, 2017 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-28603882

RESUMO

The nickel(0)-catalyzed carbonylative cycloaddition of 1,5- and 1,6-ene-imines with carbon monoxide (CO) is reported. Key to this reaction is the efficient regeneration of the catalytically active nickel(0) species from nickel carbonyl complexes such as [Ni(CO)3 L]. A variety of tri- and tetracyclic γ-lactams were thus prepared in excellent yields with 100 % atom efficiency. Preliminary results on asymmetric derivatives promise potential in the synthesis of enantioenriched polycyclic γ-lactams.

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