RESUMO
Anaplasmosis and ehrlichiosis are important tick-borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co-infections in sheep in Turkey.
Assuntos
Anaplasma ovis , Ehrlichia chaffeensis , Rhipicephalus , Animais , Ovinos/genética , Rhipicephalus/genética , Ehrlichia chaffeensis/genética , Ehrlichia canis/genética , Anaplasma ovis/genética , Turquia/epidemiologia , RNA Ribossômico 16S/genética , Filogenia , DNARESUMO
Cryptosporidium spp., and Giardia duodenalis are intestinal protozoan parasites known to infect humans and various animals and cause diarrhea. This study aimed at determining the prevalence and genotype of Cryptosporidium spp. and Giardia duodenalis in sheep in different locations of Siirt province. The fecal material for this study was collected from 500 sheep in different locations of Siirt province, Turkey. Fecal samples obtained from sheep were examined for Cryptosporidium spp. by Kinyoun Acid Fast staining and the Nested PCR method. Microscopic and Nested PCR methods revealed a prevalence of 2.4% (12/500) and 3.6% (18/500), respectively. Sequence analysis revealed the presence of C. ryanae, C. andersoni, and zoonotic C. parvum. In terms of Giardia duodenalis, 8.4% (42/500) and 10.2% (51/500) prevalence was determined using Nativ-Lugol and Nested PCR methods, respectively. Using sequence analysis, zoonotic assemblages A and B as well as assemblages E and D were detected. As a result of this study, both the prevalence of Cryptosporidium spp. and Giardia duodenalis and the presence of species that appear to be host-specific, as well as those known to be zoonotic, were revealed. A large-scale study is needed to understand the impact of these agents on sheep farming and their consequences on human health.
Assuntos
Criptosporidiose , Cryptosporidium , Giardia lamblia , Doenças dos Ovinos , Humanos , Animais , Ovinos , Giardia lamblia/genética , Turquia/epidemiologia , Criptosporidiose/epidemiologia , Cryptosporidium/genética , Genótipo , Doenças dos Ovinos/epidemiologiaRESUMO
Blastocystis spp. is a parasite that causes intestinal infection in humans and other animals. A few studies have been performed in Turkey on the distribution of Blastocystis in cattle. In this study, fecal samples were collected from 100 calves and subjected to analysis based on an SSU rRNA gene fragment. The overall prevalence of the disease was determined as 15% (15/100). This rate was 14.04% for females and 16.28% for males. In addition, three Blastocystis subtypes were identified: ST10, ST14, and novel subtypes ST25. To our knowledge, the ST25 subtype was reported with this study for the first time in Turkey. The nucleotide sequences (OM920832-OM920839) obtained in this study were deposited in GenBank. The results obtained will be useful for a better understanding of the epidemiology of Blastocystis spp., and its effects on public health.
Assuntos
Blastocystis , Animais , Bovinos , Feminino , Masculino , Blastocystis/genética , Fezes , Turquia/epidemiologiaRESUMO
A total of 508 fish specimens belonging to 19 species collected in the coast of Sile region of the Black sea were examined to detect the presence of endohelminths. Of this, 357 (70.27%) were found to be infected with parasites. 15 distinct helminths species were recovered including four species of nematodes, seven digenean species, one species of cestodes and three species of acanthocephalans. It was also determined that the species of nematode Hysterothylacium aduncum was the most common parasite and the most diverse endohelminth fauna was found in Gobius niger and Solea vulgaris, with five species. Furthermore, it should be noted that Capillaria gracilis is reported for the first time from the Turkish coasts. The infection rates, hosts, and parasites are listed in this paper.
