Decorated magnetic nanoparticles with polyvinyl alcohol brushes modified with metal chelate affinity groups for purification of proteins.

Refining is a serious challenge in the production of recombinant proteins, and this study proposes a new and easy strategy for the synthesis of magnetic affinity nanoparticles. First, the nanoparticles attached to polyvinyl alcohol brushes were synthesized, and then the hydroxyl groups of this polymer were converted to chelate groups. The nanoparticles were examined by (SEM) scanning electron microscopy, (TEM) transmission electron microscopy, (DLS) dynamic light scattering, (FT-IR) Fourier transform infrared, (XRD) X-ray powder diffraction, (VSM) vibrating sample magnetometry, and (TGA) thermal gravimetric analysis. The results confirm that uniform and spherical magnetic polymer nanoparticles with high magnetization and superparamagnetic properties were successfully synthesized. The S100A9 protein, a His-tagged recombinant protein, was expressed and purified using the synthesized nanoparticles. According to the (SDS-PAGE) sulfate-polyacrylamide gel electrophoresis results, there is a high degree of resolution in protein separation. The synthesized nanoparticles have a high protein binding capacity of about 208 mg of protein per gram of nanoparticles.

Strategies for the direct oxidative esterification of thiols with alcohols.

This review paper provides an overview of the main strategies for the oxidative esterification of thiols with alcohols. The review is divided into two major parts according to final products. The first includes the methods for the synthesis of sulfinic esters, while the second contains the procedures for the fabrication of sulfonic ester derivatives.

Vicinal halo-trifluoromethylation of alkenes.

Both trifluoromethyl and halide groups are widely found in medicinally and pharmaceutically important compounds and, moreover, organohalides are commonly used as versatile intermediates in synthetic organic chemistry. Due to their prevalence and easy accessibility, alkene halo-trifluoromethylation provides a convenient way to install these valuable functionalities in complex targets. In this review, we summarize recent advances and achievements in this fast-growing research field. For clarity, the reactions were classified according to the type of halogen atom.