RESUMO
The effect of the homogenates from different developmental stages of the nematode Protostrongylus rufescens on mitochondrial and lipid bilayer membranes has been studied. The homogenate of P. rufescens affects efficiently the cell energy by the inhibition of the mitochondrial respiration in the metabolic state V3, uncouples oxidative phosphorylation and affects the functions of mitochondria at the level of cyclosporine A-sensitive pore by making it highly permeable. Moreover, the nematode homogenate at the concentration of 1 mkg/ml increases efficiently the integral permeability of lipid bilayer membranes. An increase in this permeability is connected apparently with the formation of single ion channels. The channels of lipid bilayer membranes induced by the nematode homogenate show cation selectivity.
Assuntos
Bicamadas Lipídicas/metabolismo , Mitocôndrias Hepáticas/metabolismo , Membranas Mitocondriais/metabolismo , Nematoides/fisiologia , Extratos de Tecidos/farmacologia , Animais , Cátions/metabolismo , Fosforilação Oxidativa , Permeabilidade , Ratos , Ovinos , Canais de Ânion Dependentes de Voltagem/metabolismoRESUMO
The investigation of the effects of glycyrrhetic acid on spontaneous secretion of transmitter from neurotermination of the frog neuromuscular synapses showed that this substance, unlike glycyrrhizic acid, increased the frequency and amplitude of m. e. p. p. in a dose-dependent manner. The effect of glycyrrhetic acid is observed in calcium-free solution which may indicate ability of glycyrrhetic acid to mobilize calcium from inner cell pools. The effects of glycyrrhetic acid, in particular increasing the frequency of the m. e. p. p., testify that glycyrrhetic acid acts on the level of presynaptic membrane.
Assuntos
Ácido Glicirretínico/farmacologia , Placa Motora/metabolismo , Neurotransmissores/metabolismo , Animais , Cálcio/metabolismo , Ácido Glicirrízico/farmacologia , Potenciais da Membrana , Placa Motora/efeitos dos fármacos , Placa Motora/fisiologia , Rana temporariaRESUMO
It is shown that menadion increases the rate of Ca2+ efflux from mitochondria which decreases in the presence of ditiotreitol. Menadion at concentration of 50 microM induces Ca2+ efflux from mitochondria and its effect on synaptic transmission is discussed.
Assuntos
Cálcio/metabolismo , Mitocôndrias Hepáticas/efeitos dos fármacos , Terminações Nervosas/efeitos dos fármacos , Neurotransmissores/metabolismo , Vitamina K/farmacologia , Animais , Permeabilidade da Membrana Celular/efeitos dos fármacos , Ditiotreitol/farmacologia , Técnicas In Vitro , Membranas Intracelulares/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Terminações Nervosas/metabolismo , Junção Neuromuscular/efeitos dos fármacos , Junção Neuromuscular/metabolismo , Rana temporaria , RatosRESUMO
In vivo thyroid hormones control the binding to mitochondria of low molecular weight water-soluble cytoplasmic mediators that are capable to induce oxidative phosphorylation uncoupling, by increasing the sensitivity of mitochondria to the effects of these mediators. In hyperthyroid rat liver mitochondria cytoplasmic mediators stimulate the phosphate-dependent transport of K+ and H+ in a greater degree than in liver mitochondria of control rats. The increase in the oxidative phosphorylation uncoupling by cytoplasmic mediators is one of mechanisms of thermogenesis stimulation by thyroid hormones.
Assuntos
Hidrogênio/metabolismo , Mitocôndrias Hepáticas/metabolismo , Potássio/metabolismo , Hormônios Tireóideos/fisiologia , Animais , Transporte Biológico , Membrana Celular/metabolismo , Citoplasma/química , Masculino , Peso Molecular , Fosforilação Oxidativa , Fosfatos/metabolismo , RatosRESUMO
It has been shown by using o-phthalic acid--a concurrent inhibitor of the transport of oxalacetic acid in mitochondria that the effect of the latter on the mechanism of 2H+/Ca2+-metabolism is realized at the inner side of the inner mitochondrial membrane. Oxalacetic acid was shown to induce not only the release of Ca2+ ions but also those of strontium and manganese accumulated in the mitochondria (100-150 nmol/mg of protein). Mechanism of the effect of oxalacetic acid on permeability of the mitochondria membranes are discussed.
Assuntos
Cálcio/metabolismo , Hidrogênio/metabolismo , Mitocôndrias Hepáticas/metabolismo , Oxaloacetatos/farmacologia , Animais , Ligação Competitiva , Transporte Biológico , Técnicas In Vitro , Membranas Intracelulares/metabolismo , Cinética , Mitocôndrias Hepáticas/efeitos dos fármacos , Oxaloacetatos/metabolismo , Permeabilidade , Ácidos Ftálicos/farmacologia , RatosRESUMO
It has been found earlier in Lehninger's Laboratory [24] that oxidation of endogenous PN mitochondria induced by oxalacetic acid (OAA) brings about the release of accumulated Ca2+. Analysis of possible mechanism of this phenomenon has shown that OAA has protonophore activity on mitochondria and BPM unlike the malate--the product of enzymic reduction of OAA in the presence of NAD . H. OAA-induced permeability of BPM and mitochondria membranes for H+ is observed at pH 7.5-8.0 and is insignificant at pH 7.0 and lower. Possible function of OAA as a soft uncoupler-protonophore controlled by metabolism is discussed, in particular--in connection with Ca2+ transport in mitochondria.