Reducing ammonia emission by aluminum sulfate addition in litter and its influence on productive, reproductive, and physiological parameters of dual-purpose breeding hens.

This research investigated the impact of aluminum sulfate (AS) as amendment to different types of litter (new, reused, and mixed litters) for reducing ammonia emission and improving productive performance of local dual-purpose breeding hens. A total of 450 hens and 60 cocks from the Inshas strain were randomly assigned to six groups (five replicates each of 15 hens + 2 cocks) raised in pen floor furnished with a wheat straw litter. The groups included: (1) new, (2) reused, (3) mixed (50% new + 50% reused) litter; the other groups (4, 5 and 6) were respectively housed on the same litter as groups 1, 2 and 3 but with the addition of 495 g of AS/m2 litter. The feed conversion ratio was better for layers raised on new litter with or without AS than other groups. Different kinds of litter had different moisture (p < 0.05) and pH (p < 0.05) values. Birds raised on litter types treated with AS significantly (p < 0.05) decreased intestinal pH and decreased total bacterial count compared to the same litter types without AS at the end of the experiment. Birds raised on new litter supplemented with AS had the highest plasma T3, total protein, globulin, Hgb, and PCV% and the lowest levels of uric acid and cholesterol at the end of the experimental period. Therefore, litter amendment with AS, also the mixed or reused one, could be recommended to reduce ammonia and, in turn, increasing plasma T3 and decreasing total bacterial count, leading to increasing bird's performance.

Aspergillus awamori positively impacts the growth performance, nutrient digestibility, antioxidative activity and immune responses of growing rabbits.

In this study, we explored the effects of dietary administration of Aspergillus awamori on the growth, intestinal histomorphology, immune parameters and antioxidant enzyme activity of growing rabbits. The rabbits of 5 weeks of age (body weight, 855 ± 5.53 g) were allotted into four groups (12 rabbits per group) and fed four experimental diets enriched with A. awamori at 0, 50, 100 and 150 mg per kg diet for 8 weeks. After the feeding trial, an equal number of male APRI rabbits (3 months old; 4 groups, 5 rabbits per group) were housed in metabolism cages and fed the test diets for 14 days for the digestibility trial. All A. awamori-enriched diets induced a significant increase in the average body weight and weight gain of rabbits and significantly decreased the feed conversion ratio. In rabbits fed with A. awamori at 100 or 150 mg per kg diet, protein, lipid and fibber digestibility coefficients significantly increased compared with the control group. Intestinal villi measurements (length and thickness) were also enhanced in all rabbits fed with A. awamori. Adding A. awamori as a food supplement generally did not affect rabbit haematology and blood biochemistry values; however, at 150 mg per kg diet, it significantly increased the levels of haemoglobin and total protein, as well as red blood cell count. Furthermore, all enriched diets significantly increased rabbits' phagocytic activity and their phagocytic index. Rabbits fed with A. awamori also showed decreased malondialdehyde but increased catalase activity. In conclusion, A. awamori administered as feed supplement at 100-150 mg per kg of growing rabbits' diet enhances their growth, intestinal health and nutrient digestibility, and it raises the levels of their immune and antioxidative responses.

Influence of Oyster Mushroom Waste on Growth Performance, Immunity and Intestinal Morphology Compared With Antibiotics in Broiler Chickens.

Oyster mushroom waste (OMW) is a by-product of the agriculture industry with valuable antimicrobial, antioxidant, antifungal, and prebiotic properties. This by-product might be a useful alternative to antibiotic growth stimulators in poultry nutrition. The purpose of this research was to test the impact of OMW on the immune responses and on the morphology of intestine of broiler chickens. Four dietary therapies with five replicas of 15 birds in each, totalling 300 day- Ross 308 broiler chickens, were utilized in this study. Control chickens were fed a mixed diet that included a maize-soybean meal complemented by 1 and 2% OMW in addition to the basal diet. Furthermore, Enramycin (125 g/kg) was added to the control diet as an antibiotic. Throughout this experiment, performance was studied as well as the immune response to the Newcastle Disease Virus (NDV) and intestinal morphological traits. A substantial surge was noted in body weight gain (BWG) and feed intake (FI) of chickens after the addition of 1% OMW (p ≤ 0.05). In contrast, feed supplementation with 2% OMW, compared with the control diet, produced no noteworthy increase in BWG or the feed conversion rate (FCR). Antibiotic addition, on the other hand, increased serum cholesterol (p ≤ 0.05). After 42 days, neither OMW nor antibiotic addition affected organ mass. In contrast, antibiotic addition reduced the small intestine percentage, crypt depth and villus height (p ≤ 0.05). The Newcastle disease vaccine (NDV) antibody titer improved after feed supplementation with 1% OMW comparing with the control and antibiotic diet group. Furthermore, OMW supplementation decreased the heterophil-to-lymphocyte H/L ratio (p ≤ 0.05). The use of OMW led to a reduction in the malondialdehyde (MDA) content of the breast and liver and an increase in glutathione peroxidase. It helped to reduce glutathione, glutathione reductase, and glutathione S-transferase. In conclusion, the impact of OMW were dose-dependent, and the use of 1% OMW in broiler diets enhanced their growth and immunity. Nonetheless, supplementation with 2% OMW produced conflicting results.

Dietary Moringa oleifera improves growth performance, oxidative status, and immune related gene expression in broilers under normal and high temperature conditions.

The aim of this study is to evaluate the effects of Moringa oleifera (MO) on the performance, antioxidative status, and immune related gene expression in broilers raised under normal or heat stress conditions. Broiler chickens were distributed into 4 groups and fed diets with dietary MO at 0% or 5% (MO0 or MO5) and raised under ambient temperature 22 ±â€¯1 °C (N) or 35±1 °C (HS). HS conditions negatively affected the weight gain and FCR, while feeding MO exhibited beneficial effects especially under HS conditions. Triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL) levels were significantly (P < 0.05) higher in chickens raised in HS conditions and fed the basal diet than those in normal condition and fed with or without MO, while MO decreased triglycerides and total cholesterol levels in normal and HS conditions. Blood high-density lipoprotein cholesterol (HDL) was significantly decreased in broilers raised in HS conditions and fed diets without MO, while MO increased HDL level. Blood glutathione peroxidase (GSH-Px) was significantly (P < 0.05) decreased in broilers raised in HS conditions and fed the basal diet without MO. mRNA expression of GSH-Px was significantly (P < 0.05) downregulated in broilers raised in HS conditions and fed diets without MO. Broilers under normal or HS conditions and fed the basal diet exhibited significantly (P < 0.05) downregulated mRNA expressions of superoxide dismutase (SOD) and catalase (CAT) compared to chickens under normal conditions and fed MO. Liver and muscle thiobarbituric acid reactive substance (TBARs) were significantly (P < 0.05) increased in broilers under HS conditions and fed diet without MO. The expressions of interleukins (IL2 and IL6) were significantly (P < 0.05) downregulated in broilers under normal or HS conditions and fed diets without MO. To sum up, HS conditions depressed the performance, antioxidative status, and immune related gene expression in broilers, while MO obviously alleviated these negative effects in broilers.