Infection of guppies (Poecilia reticulata) with the Asian fish tapeworm Schyzocotyle acheilognathi in an urban stream in Brazil.

Schyzocotyle acheilognathi is a fish tapeworm native to Asia but has been reported as an alien species on practically all other continents. Its invasive potential is due to its low host specificity and high adaptability to different environments, and its spread to new areas can result in economic and ecological impacts. Studies reporting this species in South America are still scarce, indicating the need to monitor its dispersion to new areas. Herein, tapeworms found in guppies, Poecilia reticulata, from an urban stream located in Belo Horizonte, Minas Gerais, Brazil, in April 2021 were subjected to morphological and molecular characterization. As a result, 5/13 (38.5%) of the P. reticulata specimens evaluated were infected with intestinal tapeworms. It was verified a mean intensity of infection of 7.8 (1-25) and a mean abundance of infection of 3 (0-25). The morphology of the cestodes obtained was compatible with that of S. acheilognathi. Moreover, genetic analysis based on cytochrome c oxidase subunit 1 gene (Cox-1) revealed 97.89-99.77% similarity to isolates of this species from different localities. The possibility that S. acheilognathi is expanding to new regions of South America is discussed.

Philophthalmus gralli in domestic waterfowl: An environmental study in an urban area from Brazil.

Philophthalmosis is a zoonotic ocular disease that affects mainly domestic and wild avian species in different parts of the world. However, aspects related to its epizoology in domestic animals are not completely known. In the present study, 47 Muscovy duck (Cairina moschata), 8 mallards (Anas platyrhynchos platyrhynchos) and 6 African geese (Anser cygnoides), free-living in an urban lake in Belo Horizonte, Minas Gerais, Brazil, were evaluated for eye fluke infection. The animals were manually contained, and the conjunctival sac and nictitating membrane were evaluated by direct ophthalmic examination (DOE), in which the fluke burden was estimated by a semiquantitative scale. Moreover, the conjunctival sac lavage (CSL) technique was applied for the search of parasite eggs. When possible, the detected trematodes were carefully removed for fixed for taxonomic identification. In order to characterize the parasite-host-environmental system, malacological collections were performed at the locality, and the collected intermediate host mollusks (Melanoides tuberculata) were evaluated for infection with larval philophthalmids. In addition, wild vertebrates found dead in the locality, including one common gallinule (Gallinula galeata) and two capybaras (Hydrochoerus hydrochaeris), were necropsied for the search of eye flukes. Samples from the developmental stages obtained from the hosts were subjected to morphological and molecular studies (amplification and sequencing of a partial region of the Nad-1 gene). In the DOE, the presence of eye flukes was verified in 67% (4/6), 31% (15/47) and 25% (2/8) in geese, Muscovy ducks and mallards, respectively. In most cases, the birds showed mild parasitism (1-5 trematodes), with no apparent eye damage, characterizing a subclinical infection. In only one goose, which had a high fluke burden (20 trematodes), it was possible to obtain eggs by CSL. Regarding the malacological survey, 73/4545 (1.6%) specimens of M. tuberculata evaluated were found infected with megalurous cercariae, compatible with representatives of the family Philophthalmidae. Additionally, 147 flukes were recovered from a specimen of G. galeata found dead at the lake. The morphological and molecular study of parasites obtained from different hosts evaluated in this study enabled the identification of Philophthalmus gralli, a species native from Asia and introduced in the Americas. Factors such as the wide distribution of the intermediate host and the participation of domestic and wild reservoirs in the parasite transmission chain may favor the occurrence of parasite spillover from wild to domestic birds. Furthermore, the occurrence of the life cycle of P. gralli in an urban waterbody serves as an alert to the possibility of human cases of this zoonotic eye parasite.