Crustal Structure of the Collision-Subduction Zone in South of Iran Using Virtual Seismometers.

Improving the resolution of seismic tomography by adding virtual seismometers is an ambitious aim in regions with poor instrumental coverage. In this study, inter-event empirical Green's functions (EGFs) were retrieved using cross-correlation of the vertical component of 630 earthquakes with M ≥ 4 which occurred around the collision-subduction transition zone in south Iran. To extract reliable inter-event EGFs and obtain stable tomographic results, we used about 1300 event pairs with good signal-to-noise ratio, each pair well aligned to a seismic station. Our results show that the retrieved inter-event EGFs agree well with those obtained from earthquakes in similar paths. The inverted velocity model presents two main layers including upper crust (up to ~16 km) and middle crust (deeper than ~18 km) in both sides of the Minab-Zendan-Palami transition zone. The upper crust contains two main layers: sedimentary and basement layers with thicknesses ~6 and ~10 km, respectively. Moreover, the main faults cause lateral variations in these main layers. The difference between the average velocities of the middle crust, between the collision and subduction zones, is about 0.5 km/s, delimited by faults. Also, an area with a 30 km width along these faults can be defined as the collision-subduction transition zone.

The 2009 earthquake, magnitude mb 4.8, in the Pantanal Wetlands, west-central Brazil.

The main goal of this paper is to characterize the Coxim earthquake occurred in June 15th, 2009 in the Pantanal Basin and to discuss the relationship between its faulting mechanism with the Transbrasiliano Lineament. The earthquake had maximum intensity MM V causing damage in farm houses and was felt in several cities located around, including Campo Grande and Goiânia. The event had an mb 4.8 magnitude and depth was 6 km, i.e., it occurred in the upper crust, within the basement and 5 km below the Cenozoic sedimentary cover. The mechanism, a thrust fault mechanism with lateral motion, was obtained by P-wave first-motion polarities and confirmed by regional waveform modelling. The two nodal planes have orientations (strike/dip) of 300°/55° and 180°/55° and the orientation of the P-axis is approximately NE-SW. The results are similar to the Pantanal earthquake of 1964 with mb 5.4 and NE-SW compressional axis. Both events show that Pantanal Basin is a seismically active area, under compressional stress. The focal mechanism of the 1964 and 2009 events have no nodal plane that could be directly associated with the main SW-NE trending Transbrasiliano system indicating that a direct link of the Transbrasiliano with the seismicity in the Pantanal Basin is improbable.

Purification and characterisation of Azospirillum brasilense N-truncated NtrX protein.

The NtrX protein has been identified as a transcriptional activator of genes involved in the metabolic control of alternative nitrogen sources, acting as a member of a two-component regulatory system. The in silico analysis of the NtrX amino acid sequence shows that this protein contains an N-terminal receiver domain, a central AAA+ superfamily domain and a C-terminal DNA binding domain. To over-express and purify this protein, the ntrX gene of Azospirillum brasilense lacking the first eight codons was cloned into the vector pET29a+. The NtrX protein was over-expressed as an S.Tag fusion protein induced by l-arabinose in the Escherichia coli strain BL21AI and purified by ion exchange and affinity chromatography. The ATPase activity of NtrX was measured by coupling the ATP conversion to ADP with NADH oxidation. The ATPase activity of NtrX was stimulated in the presence of A. brasilense sigma(54)/NtrC-dependent promoter of the glnBA gene. Phosphorylation by carbamyl-phosphate also stimulated ATPase, in a manner similar to the NtrC protein. Together our results suggest that NtrX is active in the phosphorylated form and that there may be a cross-talk between the NtrYX and NtrBC regulatory systems in A. brasilense.

Repressor mutant forms of the Azospirillum brasilense NtrC protein.

The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.