RESUMO
The results ofMTHFR gene C(677)-->T (rs1801133) polymorphism determined in 170 patients with ischemic atherothrombotic stroke (IATS) and 124 healthy subjects (control group) are presented in the paper. It has been shown that in patients with IATS, the frequencies of main homozygotes (CC), heterozygotes (CT) and minor homozygotes (TT) are 52.4, 35.9, 11.8% (in control--46.0, 48.4, 5.6%, P = 0.044 by chi2-test). TT homozygotes have a greater chance of developing IATS than carriers of main C-allele (CT + CC) (OR = 2.3, CI = 0.911-5.449, P = 0.049). In the representatives of the Ukrainian population there is a relationship between the frequency of MTHFR gene C(677)-->T polymorphism genotypes and the risk of IATS. This connection is manifested in male patients, in persons with normal blood pressure, and in people who do not have the habit of smoking. The sex of the patients, body mass index, blood pressure and smoking affect the level of the studied polymorphism association with stroke.
Assuntos
Isquemia Encefálica/genética , Infarto da Artéria Cerebral Anterior/genética , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Índice de Massa Corporal , Isquemia Encefálica/enzimologia , Isquemia Encefálica/etnologia , Isquemia Encefálica/patologia , Feminino , Frequência do Gene , Heterozigoto , Homozigoto , Humanos , Infarto da Artéria Cerebral Anterior/enzimologia , Infarto da Artéria Cerebral Anterior/etnologia , Infarto da Artéria Cerebral Anterior/patologia , Masculino , Metilenotetra-Hidrofolato Redutase (NADPH2)/metabolismo , Pessoa de Meia-Idade , Fatores de Risco , Fatores Sexuais , Fumar , Ucrânia , População BrancaRESUMO
G-7A polymorphism (rs1800801) of matrix Gla protein (MGP) gene in 170 patients with ischemic atherothrombotic stroke (IATS) and in 124 persons of the control group was determined. It was shown that in patients with IATS the distribution of the major allele homozygotes, heterozygotes and minor allele homozygotes was 35.9; 48.8; 15.3% (in control--43.5; 50.0; 6.5%, P = 0.051 by chi2-test). Significant differences in the distribution of genotypes were revealed only in women (P = 0.022). Odds ratio for minor allele homozygotes (A/A) versus major allele carriers (G/A+G/G) was 2,618 (P = 0.023), while in women it was equal to 6,645 (P = 0.015). In patients with A/A genotype the values of blood coagulation parameters (prothrombin time) indicated increased predisposition to hypercoagulability. The results obtained suggest that the A/A-variant of MGP gene is associated with an increased risk of IATS in female persons of the Ukrainian population and may be related to blood hypercoagulability and thrombi formation.
Assuntos
Isquemia Encefálica/genética , Proteínas de Ligação ao Cálcio/genética , Proteínas da Matriz Extracelular/genética , Arteriosclerose Intracraniana/genética , Polimorfismo de Nucleotídeo Único , Acidente Vascular Cerebral/genética , Trombofilia/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Isquemia Encefálica/complicações , Isquemia Encefálica/patologia , Feminino , Frequência do Gene , Genótipo , Heterozigoto , Homozigoto , Humanos , Arteriosclerose Intracraniana/complicações , Arteriosclerose Intracraniana/patologia , Masculino , Pessoa de Meia-Idade , Tempo de Protrombina , Fatores de Risco , Fatores Sexuais , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/patologia , Trombofilia/complicações , Trombofilia/patologia , Ucrânia , Proteína de Matriz GlaRESUMO
Matrix Gla protein (MGP) gene allelic polymorphism in 170 patients with ischemic atherothrombotic stroke (IATS) and in 124 healthy people was determined. It was shown that in patients with IATS interrelation of main homozygotes, heterozygotes and minor homozygotes is 61,2, 31,2, 7,6% for T-138 --> C (rs1800802) promoter polymorphism (in control -59.7, 35.6, 4.8%, P = 0.521 by chi2-test); 35.9, 48.8, 15.3% for G(-7) --> A (rs1800801) polymorphism of promotor (in control--43.5, 50.0, 6.5%, P = 0.051); 39.4, 48.8, 11.8% for Thr83 --> Ala (rs4236) polymorphism of 4 exon (in control - 34.7, 53.2, 12.1%, P = 0.701). Significant differences in the frequency of G(-7) --> A polymorphism between patients with IATS and control group were revealed only for women (P = 0.022). The results obtained suggest that the A/A-variant of MGP gene promotor (G(-7) --> A polymorphism) is associated with an increased risk of IATS in female persons in the Ukrainian population.
Assuntos
Isquemia Encefálica/genética , Proteínas de Ligação ao Cálcio/genética , Proteínas da Matriz Extracelular/genética , Placa Aterosclerótica/genética , Polimorfismo de Nucleotídeo Único , Acidente Vascular Cerebral/genética , População Branca/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Isquemia Encefálica/complicações , Estudos de Casos e Controles , Éxons , Feminino , Frequência do Gene , Humanos , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica/complicações , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , Fatores de Risco , Fatores Sexuais , Acidente Vascular Cerebral/complicações , Ucrânia , Proteína de Matriz GlaRESUMO
We showed here that energy metabolism in thoracic rabbit aorta and posterior vena cava is disturbed two weeks following the induction of alloxan-induced diabetes and monoiodacetate intoxication. In the vessels studied, the alterations are manifested in the decrease in the intensity of glucose uptake, oxygen consumption, lactic acid production, and ATP resynthesis. Simultaneously, the ATP content is significantly reduced. The possible significance of these disorders in the development of Monckeberg's arteriosclerosis is discussed.
Assuntos
Aorta Torácica/metabolismo , Diabetes Mellitus Experimental/metabolismo , Metabolismo Energético/efeitos dos fármacos , Ácido Iodoacético/toxicidade , Veias Cavas/metabolismo , Trifosfato de Adenosina/metabolismo , Aloxano , Animais , Aorta Torácica/efeitos dos fármacos , Diabetes Mellitus Experimental/fisiopatologia , Glucose/metabolismo , Ácido Láctico/metabolismo , Masculino , Esclerose Calcificante da Média de Monckeberg/metabolismo , Consumo de Oxigênio/efeitos dos fármacos , Coelhos , Veias Cavas/efeitos dos fármacosRESUMO
The intensity of the lipid peroxydation (LPO) and the antioxidant enzyme activity (gluthathione peroxydase, superoxide dismutase and catalase) after monoiodacetate injection (10 mg/ kg) within 3, 7 and 14 days was examined in the arterial and venous walls of rabbits. The increase in the amount of the intermediate and final LPO products, and also the reduction in the antioxidant enzymes activity has been found in the vessels of all types. The changes in the studied parameters were more expressed in the venous vessels, than in the arteries. It is supposed that the activation of LPO is caused by the primary depression of antioxidant systems which arises as the consequence of the disorders of energy exchange in the vascular wall under the influence of monoiodacetate.
