[Use of an activated chemiluminescence method for studying the pharmacologic properties of medicinal substances]. / Ispol'zovanie metoda aktivirovannoi khemiliuminestsentsii pri izuchenii farmakologicheskikh svoistv lekarstvennykh preparatov.

When given to non-inbred male rats during 1, 2, and 3 months, aqueous extract from the above-ground Padus Grajana maxim parts was tested for its effects on the parameters of chemiluminescence-activated rat serum. The tested agent was found to increase he rate of rapid and slow chemiluminescence flashes. The level of steady-state glow considerably declined after the agent was given for a month; however, its longer use increased the steady-state chemiluminiscence and by the end of the 3rd month it was equal to that of control animals. Whether the activated chemiluminescence technique can be used to evaluate the effect of biologically active substances on cell structures, to refine some aspects of the mechanism of action of drugs is discussed on in terms of the findings.

[Antioxidative properties of glycyrrhyzic acid salts and their effect on the liver monooxygenase system]. / Issledovanie antiokislitel'nykh svoistv solei glitsirrizinovoi kisloty i ikh vliianiia na mikrosomal'nuiu monooksigenaznuiu sistemu pecheni.

Antioxidative activity of potassium- and sodium glycyrrhizins was detected by means of chemoluminescence procedure. As shown by the spectral studies the glycyrrhizin salts formed complex of the I type with cytochrome P-450 from rat liver microsomes, inhibited inactivation of isolated cytochrome P-450LM2 and induced hemoprotein formation after administration into rats. The preparations of glycyrrhizic acid appear to be useful for treatment of toxic impairment of liver tissue.

[Two phases in development of chemiluminescence during lipid peroxidation induced by Fe2+ ions]. / Dve fazy v razvitii khemiliuminestsentsii pri perekisnom okislenii lipidov, initsiirovannom ionami Fe2+.

After cessation of the stirring of reagents in the egg yolk lipoprotein suspension where lipid peroxidation had been initiated by ferrous ions a drop of chemiluminescence (CL) was observed after a lag period, the duration of which increased in the course of stationary-state CL development. The CL intensity was but slightly dependent on oxygen pressure in the gas phase above incubation mixture within oxygen partial pressure limits 760-40 mm Hg. These data suggest that the CL quantum yield is growing during lipid peroxidation development as a result of the formation in the system of some compounds amplifying the CL intensity and/or development of a new CL reaction whose rate depends purely on oxygen and whose photon emitters are different.

[Activation of chemiluminescence accompanying lipid peroxidation in monolayer liposome suspension]. / Aktivatsiia khemiliuminestsentsii, soprovozhdaiushchei perekisnoe okislenie lipidov v suspenzii odnosloinykh liposom.

In the presence of rhodamine J the chemiluminescence intensity of monolayer liposomes induced by ferrous ions is increased by three orders. There is no accumulation of malonic dialdehyde. It is suggested that chemiluminescence activation is related to rhodamine J interaction with the products of lipid peroxidation whose molecules are in the excited state.

[Chemiluminescence activated by rhodamine G in plasma in the presence of divalent iron ions]. / Aktivirovannaia rodaminom Zh khemiliuminestsentsiia plazmy krovi v prisutstvii ionov dvukhvalentnogo zheleza.

Rhodamine G was found to activate blood plasma chemiluminescence induced by ferrous ions. The dye in concentrations 300-500 mole/l increased chemiluminescence by an order of magnitude. The luminescence was inhibited by histidine and SOD. A conclusion may be drawn that the mechanism of the activated ferrous chemiluminescence with rhodamine G was related to superoxide anion-radicals and singlet oxygen.