[Effect of dietary linoleic acid on the platelet aggregation, fatty acid composition of platelet phospholipids and synthesis of platelet prostaglandins in rats]. / Vliianie pishchevoi linolevoi kisloty na agregatsiiu trombotsitov, zhirnokislotnyi sostav ikh fosfolipidov i sintez trombotsitarnykh prostaglandinov u krys.

Three groups of Wistar-Kyoto rats were kept on diets containing different quantity of linoleic acid (LA) in prenatal and postnatal periods up to 18 weeks age: I. less than 0.1 cal.% of LA, 2. 9.0 cal.% of LA and 3. 16 cal.% of LA. Aggregation of washed platelets with ADP and A23187, biosynthesis of prostaglandins from 14C-arachidonic acid in platelets and fatty acid composition of platelet phospholipids were studied using gas liquid chromatography. Modification of fatty acid composition of platelet phospholipids and changes in biosynthesis of platelet prostaglandins are the base for proaggregation effect of LA deficient and for antiaggregation effect of LA enriched diets. The effects of dietary LA were mediated not only via altered metabolism of fatty acids of linoleic family but also via metabolism of fatty acids of oleic and linolenic series.

[Influence of rations with various linoleic acid contents on the fatty acid composition of phospholipids in blood platelets of rats]. / Vliianie ratsionov s razlichnym soderzhaniem linolevoi kisloty na zhirno-kislotnyi sostav fosfolipidov trombotsitov krys.

Gas-liquid chromatography was used to study the fatty-acid composition of phospholipids in platelets of Wistar-Kioto rats which received semisynthetic rations with varying content of linoleic acid, 1 week prenatally and 18 weeks postnatally. In ration I the level of 18:2n6 was lower than 0.1 cal%, ration II contained 9.0 cal%, and ration III -- 16.0 cal%. Ration III, as compared to ration II, induced an increase in the content of linoleic fatty acids, arachidonic fatty acid content being unchanged. Ration I, as compared to ration II induced a decrease in linoleic fatty acid content, and an increase in the content of oleic and linolenic fatty acids attended by the appearance of appreciable amounts of 20:3n9 and 20:5n3. Long-term linoleic acid deficiency induced no changes in the unsaturation degree of fatty acids in platelet phospholipids of rats given ration I. This is an evidence of high reserve possibilities of the mechanisms regulating fatty acid metabolism in the mammals.

[Characteristics of early disorders of fatty-acid composition of kidney lipids in spontaneous (hereditary) arterial hypertension]. / Kharakteristika rannikh narushenii zhirnokislotnogo sostava lipidov pochek pri spontannoi (nasledstvenno obuslovlennoi) arterial'noi gipertenzii.

Fatty acid composition of kidney lipids from spontaneously hypertensive Okamoto-Aoki rats (SHR) and normotensive Wistar rats (NR) was investigated two days after birth by gas-liquid chromatography. In SHR kidney phospholipids, as compared to the respective NR values, the content of arachidonic acid (20:4n6) was decreased and the levels of oleic (18:1n9) and docosahexaenoic (22:6n3) acids were increased; in kidney triglycerides, the content of eicosatrionoic acid (20:3n9) and all fatty acids of the linoleic acid series was decreased, except the content of a polyunsaturated long-chain product of linoleic acid metabolism, docosapentaenoic acid (22:5n6) that was increased. These changes may have developed prenatally as a result of genetic peculiarities of SHR.

[Content of prostaglandin biosynthesis precursors in the kidney lipids in the development of spontaneous arterial hypertension]. / Soderzhanie predshestvennikov biosinteza prostaglandinov v lipidakh pochek pri razvitii spontannoi arterial'noi gipertenzii.

The content of linoleic (18 : 2 omega 6), arachidonic; (20 : 4 omega 6) and eicosatrienic (20 : 3 omega 9) acids was studied and compared in lipids of the renal cortex and papilla of spontaneously hypertensive Okamoto-Aoki rats (SHR) and normotensive Wistar rats (NR) aged 10 days, 1, 2 and 4 months. The level of 18 : 2 omega 6 in lipids of the renal cortex and papilla of SHR was lower at the prehypertensive stage (10 days) and at the stage of persistent hypertension as compared to that seen in NR. The content of 20 : 4 omega 6 in phospholipids and triglycerides of the cortex and in phospholipids of the papilla of SHR did not differ from that in NR. In triglycerides of the papilla of SHR of all age groups, the content of 20 : 4 omega 6 was higher than in NR. In phospholipids of SHR cortex, the content of 20 : 3 omega 9 was higher than that in NR. As for lipids of the papilla, 20 : 3 omega 9 was not detectable either in NR or SHR. It is concluded that the data obtained are of interest for the characteristics of the renal prostaglandin system in SHR.

[Effect of a diet with various amounts of essential fatty acid on the fatty acid spectrum of lipids in the kidneys in spontaneously hypertensive rats]. / Vliianie diet s razlichnym soderzhaniem nezamenimykh zhirnykh kislot na zhirnokislotnyi spektr lipidov pochek spontanno-gipertenzivnykh krys.

Fatty acid composition of phospholipids and triglycerides was studied by means of gas chromatography in kidney of rats with spontaneous hypertension, kept on rations containing various amounts of essential fatty acids. Deficiency of essential fatty acids caused an increase in content of oleic acid (18:1 omega 9) and of its metabolite--eucosetrienic acid (20:3 omega 9). Feeding of the animals with large amounts of linoleic (18:2 omega 6) and linoleic (18:3 omega 3) acids led to an increase of their content in kidney lipids as well as to elevation of prostaglandin precursors level. With an increase in deficiency of linoleic acid activity of delta 5-desaturation of its derivatives was decreased. Decrease in the rate of arterial pressure was observed in those spontaneously hypertensive rats, in kidney of which the content of linoleic, linoleic acids and eucosepentanic acid of the linoleic species (20:5 omega 3) was increased after the alimentary treatments.

[Effect of Vibrio cholerae filtrate on the adenyl cyclase system in vitro]. / Vliianie kholernogo fil'tra na adenilattsiklaznuiu sistemu in vitro.

An in vitro model using homogenates of the rat intestine and liver for studying the V. Cholerae culture filtrate effect on the adenylate cyclase system is proposed. Optimal conditions for the adenylate cyclase functioning have been investigated for this model. It was shown that V. Cholerae filtrate induces a stable activation of adenylate cyclase and does not change the activity of cyclic AMP phosphodiesterase. It was also established that the activity of phosphodiesterase in the intestinal homogenate is about 2.5 fold higher than that in the liver homogenate. The model may be considered promising for investigation of the mechanism of cholerae toxin action.