RESUMO
An assay has been developed to analyse cell surface properties using agarose beads derivatized with amino acids, sugars, proteins, and other molecules. The assay is simple and rapid and is useful to identify new cell surface markers. Various species and strains of yeast, paramecium, and Euglena were tested for their ability to bind to over 100 types of derivatized beads. A variety of specificity studies were performed in order to understand the nature of cell-bead binding. Our results indicate that cell-bead binding is often specific enough to distinguish between configurational isomers and spacer sizes and can be blocked by addition of specific molecules to the incubation medium. In some cases, different species or strains differed only by their binding to a single bead type. This simple and rapid assay may help to uncover new cell surface receptors and may lead to the development of clinically useful compounds for therapeutic applications.
Assuntos
Membrana Celular/metabolismo , Sefarose/metabolismo , Aminoácidos/metabolismo , Animais , Metabolismo dos Carboidratos , Adesão Celular , Euglena gracilis/metabolismo , Euglena gracilis/ultraestrutura , Lectinas/metabolismo , Proteínas de Membrana/metabolismo , Microesferas , Conformação Molecular , Paramecium/metabolismo , Paramecium/ultraestrutura , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/ultraestrutura , Sensibilidade e Especificidade , Propriedades de SuperfícieRESUMO
A patient with acute myelomonocytic leukemia was found to have trisomy 20 in his bone marrow cells. The patient achieved a complete response to standard antileukemic therapy. The literature is reviewed in regard to this abnormality.
