RESUMO
Combination of the usual techniques yielded 70-80 p.c. pure apo- and iron-transferrin preparations, whose analysis revealed the presence of antigenically identical transferrin fractions differing from one another in respect of electrophoretic motility and sedimentation contrast. No similar modifications of transferrin have previously been reported.
Assuntos
Transferrina/isolamento & purificação , Eletroforese em Gel de Ágar , Humanos , Imunodifusão , Imunoeletroforese , Imunoeletroforese Bidimensional , Ferro/metabolismo , Transferrina/análise , Transferrina/imunologiaAssuntos
Carcinoma in Situ , Complicações na Gravidez , Neoplasias do Colo do Útero , Feminino , Humanos , Recém-Nascido , Masculino , GravidezRESUMO
The authors have compared the biochemical, immunochemical and immunobiological properties of a chloramphenicol-resistant S. typhi strain isolated during the typhoid fever epidemic in Mexico of 1972 and those of a chloramphenicol-sensitive strain (S. typhi Ty2). They have found no difference in the chemical composition of the two strains. The immunobiological investigations have shown, that the chloramphenicol-resistant strain contains less Vi antigen, it is less virulent on mice, and its active and passive mouse-protective ability is lower than that of the chloramphenicol-sensitive S. typhi Ty2 strain. These findings supported by the result of the electrophoretic analysis suggest that the chloramphenicol-resistant strain is a VW strain, while the chloramphenicol-sensitive strain is a full V strain; the quantitative difference found between the immunogenicity of the two strains to the advantage of the chloramphenicol-sensitive strain may probably be explained by this fact. On the basis of their immunological investigations the authors are of the opinion, that vaccines prepared from chloramphenicol-sensitive S. typhi strains (provided these are full V strains, as e.g. the S. typhi Ty2 strain) will protect most likely also man against infection due to chloramphenicol-resistant S. typhi strains.
Assuntos
Cloranfenicol/farmacologia , Resistência Microbiana a Medicamentos , Salmonella typhi/efeitos dos fármacos , Testes de Aglutinação , Animais , Antibacterianos/farmacologia , Antígenos de Bactérias/análise , Feminino , Fermentação , Hexoses/análise , Masculino , Nitrogênio/análise , Fósforo/análise , Salmonella typhi/análise , Salmonella typhi/imunologia , Febre Tifoide/prevenção & controle , Vacinas Tíficas-Paratíficas/farmacologia , Vacinas Tíficas-Paratíficas/toxicidade , VirulênciaRESUMO
An immunodiffusion method combined with thin-layer chromathography was elaborated for the assessment of the molecular weight of IgM. Sera from 100 normal individuals as well as from 162 patients with different types of paraproteinaemia, 100 patients with uveitis and 55 venous umbilical cord sera were analysed. Presence of 75 IgM, besides the usual 19S IgM was detected in 21% of the normal sera, in 27% of the paraproteinaemic sera, in 30% of the uveitic sera and in 5.4% of venous umbilical cord sera. IgM level in the sera was determined by radial immunodiffusion. The authors didn't find correlation between the presence of 7S IgM and different levels of IgM.
