[The relationship between concentrations of pepsinogens in blood serum and pathohystologic parameters of stomach adenomas and adenocarcinomas].

The article demonstrates that in patients with adenomas of stomach the concentration of pepsinogen I in blood serum and the rate between pepsinogen I and pepsinogen II is decreased. In case of adenomas and adenocarcinomas of stomach the concentration of pepsinogen I and the rate between pepsinogen I and pepsinogen II are in inverse correlation relationship with indicators of proliferative activity of epithelial cells and tumor cells correspondingly. The threshold level was the rate between pepsinogen I and pepsinogen II can be used as criteria of intensity ofproliferative activity of epithelial cells of adenomas and cells of adenocarcinomas of stomach.

[Humoral factors of immunity and the mononuclear cell responses to fetoproteins in patients with pulmonary tuberculosis].

The content of cytokines, the levels of antibodies (Ab) to proinflammatory cytokines and fetoproteins (FP) in serum, as well as the effects of fitohemagglutinin and FP on the level of cell production of cytokines into the conditioned medium were studied in relation to the pattern of a response of mononuclear cells (MNC) to FP. With the positive reaction of MNC to FP, estimated by an increment of CD9* cells and detectable in grades 2-3 dysplasias, the anti-inflammatory effect with lower anti-inflammatory cytokines was shown to be achieved due to elevated Ab levels, which may compensate for the low content of IL-4 and IL-10. With FP, there was an increase in the cell production of IL-10 that is known to stimulate antibody formation in the early phase of tumor growth, as evidenced by the association of the levels of Ab to FP with the grade of dysplasia.

[Mononuclear cell response to fetoproteins in patients with pulmonary tuberculosis in dysregenerative bronchial epithelial changes].

A relationship was studied between the response of mononuclear cells (MNC) to fetoproteins (FP) and the bronchial changes in patients with pulmonary tuberculosis. The response was evaluated by the change in the relative count of CD8+ lymphocytes after MNC incubation with FP as compared with the controls. The value of the response (in scores) was compared with the pathohistological pattern of the bronchial epithelium and interstitial tissue. The studies show that the values of a MNC response to FP are criteria for the presence and absence of dysplasia as one of the types of bronchial epithelial atypia. It is not improbable that patients with chronic inflammatory processes in the lung in the presence of a positive MNC response to FP should be regarded as persons at risk for malignancies.

[The levels of T lymphocytes and thymic peptide antibodies in patients with infiltrative pulmonary tuberculosis and in those with cancer diseases]. / Soderzhanie T-limfotsitov i urovni antitel k peptidam timusa u bol'nykh infiltrativnym tuberkulezom legkikh i u bol'nykh s onkologicheskimi zabolevaniiami.

A relationship of the levels of thymic peptide antibodies to those of the population and subpopulations of T lymphocytes was studied in patients with pulmonary tuberculosis, lung or breast cancer. Indirect immunofluorescence was used to determine the count of CD3-, CD4-, CD80, CD38-, CD25-, CD71-, and CD95-lymphocytes. The levels of thymic peptide antibodies were estimated by enzyme immunoassay. Unlike cancer diseases, pulmonary tuberculosis was characterized by the combination of a significantly inverse correlation between the relative count of CD3-, CD4-, CD8-lymphocytes the levels of thymogen antibodies with a significantly direct correlation between the absolute count of CD25- and CD38-lymphocytes. The studies may recommend that the levels of thymic peptide antigens should be determined to select persons with immunodeficiency particularly those with inflammatory diseases.

[Antibodies to mycobacterial antigens in patients with pulmonary tuberculosis]. / Antitela k antigenam mikobakterii u bol'nykh tuberkulezom legkikh.

The levels of IgG and IgM antibodies to different mycobacterial antigens were studied in patients with varying pulmonary tuberculosis. The higher levels of IgG and IgM to the used antibodies were simultaneously found in patients with infiltrative tuberculosis as compared with healthy individuals. As compared to patients with fibrocavernous pulmonary tuberculosis, those with infiltrative tuberculosis had significantly lower levels of IgG antibodies to BGC sonicate and protein with a molecular weight of 40-70 kD isolated from the strain H37Rv. A correlation was found between the IgM antibodies to protein isolated from the strain H37RV and to H37RV sonicate and the degree of the activity unique to fibrocavernous pulmonary tuberculosis.

[Levels of some cytokines in infants with central nervous system damage]. / Soderzhanie nekotorykh tsitokinov u detei s porazheniem tsentralnoi nervnoi sistemy.

Two cytokines--interleukin 1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) levels have been studied in infants with CNS damage of different severity. The more was the brain lesion severity, the higher the TNF-alpha level increased. IL-1 beta levels were reduced significantly in severe brain injury caused by hypoxia. Chronic hypoxia combined with intrauterine infection and/or a risk of the latter was accompanied by imbalance of IL-1 beta and TNF-alpha secretion.

[Quantitative characteristics of the cellular link of immunity and a lymphocytic response to tumor-associated antigens in patients with tuberculosis and malignant neoplasms of the lung]. / Kolichestvennye kharakteristiki kletochnogo zvena immuniteta i reaktsiia limfotsitov na opukholeassotsiirovannye antigeny u bol'nykh pri tuberkuleze i zlokachestvennykh novoobrazovaniiakh legkikh.

The relative quantities of basic populations and subpopulations of lymphocytes and their response to tumor-associated antigens were estimated in the peripheral blood patients with tuberculosis and malignant neoplasms of the lung. In both diseases, the changes in the count of immunocompetent cells are similar and they characterize immunodeficiency typical of these conditions. A response to tumor-associated antigens was found only in patients with lung cancer, the pattern of this response depending on the grade of a tumor.

[Lymphocyte reaction and humoral immune response to brain specific protein in children with pathology of the central nervous system]. / Reaktsiia limfotsitov i gumoral'nyi immunnyi otvet na mozgospetsificheskii belok u detei s patologiei tsentral'noi nervnoi sistemy.

Blood lymphocyte reaction to brain specific protein (BSP) in vitro and anti-brain antibodies (AB) levels were studied in newborns and infants with perinatal central nervous system (CNS) damages of various severity. A relationship between severity of CNS damages and a lymphocyte reaction to BSP as well as immuno-regulative index (IRI) changes. A combination of high AB to BSP levels with IRI elevation after cells incubation with BSP was found to be a favorable prognostic sign. In contrast, IRI reduction combined with higher AB levels appeared to be an unfavorable one.

[Antibodies to bacterial antigens and autoantigens in pregnant women with kidney diseases]. / Antitela k bakterial'nym antigenam i autoantigenam u beremennykh zhenshchin s pochechnoi patologiei.

The levels of antibodies (Ab) to bacterial antigens of gram-negative enterobacteria (Reglicolipid), Streptococcus agalactiae polysaccharide, Staphylococcus aureus teichoic acid, native and denatured DNA and renal proteins in healthy pregnant women and in those with renal pathology (chronic and gestation pyelonephritis) were studied. The study revealed that the combination of an elevated levels of Ab to bacterial antigens with the elevated titers of Ab to DNA and renal proteins is indicative of acute inflammation in kidneys, and but the combination of an elevated levels of Ab bacterial antigens with the low level of Ab to DNA and renal proteins is indicative of healthy carrier state with respect to a given infective agent.

[Antibodies to Bacillus megaterium H glycoprotein in patients with lung diseases]. / Antitela k glikoproteinu Bacillus megaterium H u bol'nykh s legochnoi patologii.

Enzyme immunoassay performed in patients with malignant and benign affections of the lungs determined antibodies (Abs) to glycoprotein isolated from saprophyte microorganism Bacilus megaterium H. In pulmonary tuberculosis and nonspecific pulmonary inflammation the above Abs occurred in patients with fibrous-proliferative changes and associated neoplastic conditions. In lung cancer the Abs were detected in well and moderately differentiated tumors. Nondifferentiated and poorly differentiated variants did not exhibit any rise in Abs to glycoprotein.

[Quantitative characteristics of immunity in patients with reactivated pulmonary tuberculosis]. / Kolichestvennye kharakteristiki immuniteta u bolnykh s reaktivatsiei tuberkuleza legkikh.

Immune status was assessed in 44 patients with pulmonary re-infection tuberculosis using monoclonal antibodies. Quantitative indices of the immune status (relative amount of T-cells, T-helpers, T-suppressors, natural killers) correlated with tuberculosis activity. It is important to identify immunity-compromised subjects among tuberculous patients to rule out relapses with the help of early immunocorrection.

[Determination of antibodies to cross-reacting antigens of microorganisms in oncological diseases]. / Opredelenie antitel k perekrestno reagiruiushchim antigenam mikroorganizmov pri onkologicheskikh zabolevaniiakh.

It was studied antibodies to saprophytic microorganism Bacillus megaterium H glycoprotein in healthy, oncological, non-oncological gastrointestinal patients and animals. High level of antibodies to microbial glycoprotein in blood sera was revealed in cancer and precancer patients. Analogical results were obtained in mice A/Sn and Balb/c with inductive or transplanted tumors. It has been suggested the use of Bacillus megaterium H glycoprotein (M. m. 65-70 kD) for immunological monitoring.

[Sensitization of lymphocytes from newborn infants and young children to pathogenic and opportunistic microflora]. / O sensibilizatsii limfotsitov novorozhdennykh i detei rannego vozrasta k patogennoi i uslovno-patogennoi mikroflore.

The nucleolar test was used to study sensitization of peripheral blood lymphocytes from the newborn and infants. The test is based on the counting of cells with a clarification area around nucleoli after incubation with antigens and microbial bodies of the pathogenic and opportunistic microflora. It has been established that the peripheral blood from children with infectious-inflammatory diseases and from those vaccinated with BCG shows an increase in the sensitized lymphocyte count which is influenced by child's trophicity at birth and by administration of antibacterial therapy. The use of the nucleolar test as a diagnostic aid is advisable to identify the disease etiology.

[Correlation between altered granulocytes and visualized brain anti gens in newborn infants with pathology of the central nervous system]. / O vzaimodeistvii izmenennykh granulotsitov s vizualizirovannymi mozgovymi antigenami u novorozhdennykh s patologiei tsentral'noi

In order to reveal granulocyte sensitization in children with CNS pathology, use was made of the immunocytoadhesion test with cerebral antigens visualized by means of latex particles. The healthy children and those with encephalopathies manifested significant differences in the test as well as correspondence of the test values with the gravity of the clinical manifestations of CNS pathology. It has been noted that the mother's health status influenced the test values in neonates with encephalopathies.

[Differential microcalorimetry of the antigen-antibody reaction]. / Differentsial'naia mikrokalorimetriia reaktsii antigen-antitelo.

High sensitivity of the microcalorimetric method for the registration of immunologic reactions and prospects of the practical application of the method demand an objective interpretation of the results and an explanation of the range of thermistometry findings varying from positive to negative values. The investigations have been carried out with models with known titers of purified antigens and antibodies and with unknown antibody titers. The method has been employed in examinations of children suffering from infectious allergic diseases and from central nervous system abnormalities. The energy processes connected with the negative, zero, or positive results of the reactions, depending on the antibody and antigen concentrations, are explained.

[Morphologic changes of the nucleolar system of lymphocytes as the indicator of sensitivity to tuberculin and Toxoplasma antigen]. / Morfologicheskie izmeneniia nukleoliarnogo apparata limfotsitov kak ppkazate'l chuvstvite'lnosti k tuberkulinu i toksoplazmen- nomu antigenu.

A study was made of the nucleolar apparatus of peripheral blood lymphocytes of the newborns, infants and their mothers before and after incubation in vitro with tuberculin or toxoplasmic antigen. It was established that in the presence of sensitization to one or another antigen, there was an increase in the relative count of lymphocytes having a zone of clarification near nucleoli. In children vaccinated with BCG, such an effect was noted in 81.3% of the cases, whereas in children with verified toxoplasmosis, in all the cases. It is recommended that the method designed might be used as alternative for the complement fixation test in the diagnosis of toxoplasmosis.